LOCUS       sequence-355663-        9727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others
            and others expression of miniSOG-SL2 BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3449
FEATURES             Location/Qualifiers
     source          1..9727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5006..5326
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(7499..7521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7621..7640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7718..7736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7804..7908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7909..7977,7978..8769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8127..8146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8940..9528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9429..9448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9682..9699
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
      241 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
      301 cgatggatac gctaacaact tggaaatgaa ataagcttgg gctgcagcat gcggccgcat
      361 ttgttgaaaa aacgtacggt cttcatttaa gagtgacata gtttcttctt acatttttaa
      421 tattaatctg atgtttcaga tctgctctaa taatggctgg ttcagcacat caaaacgagt
      481 acgacttttc gcaacgacgg cattcactta tcaacatgtt caaggcgttt aaattgtaaa
      541 caatcttgtg atgcagcatt gctccaatga taatttgggt atttcatagg gtgttatttt
      601 gcatctgttt atctatgaag aagctgaaaa ctggaattat cattagagca agttcgcaag
      661 cagaccagca cacaaaataa tgcaacagaa tttggttccc aaagagctca ataaagttta
      721 cttttcacaa atgcttcaat ttccgtttct tccatttaaa ttgttctgat gttatagctt
      781 ctcgcacttt ccctccattt caatttcccg cctattttct accctcaaaa gttcctctca
      841 tagttttctt attgatgagg aacttttggg cattagcaaa attgtgccct ttgaattaag
      901 ttaaaatgaa ttaaataatc ttttattaaa gcgaattcgc atcatatttt aaatgcgcgg
      961 tattaataaa aataggttcg tcgatcaata ggtgttttca tattatccaa atacacaatt
     1021 gggcaatttg taaaacttga agaatgcggc aacgagttct gtgcaagtct ttacctttaa
     1081 tgcttccgtc atcgttagca aaaactgtgt catgaaagac aagtgtataa gattattcaa
     1141 atacaatttt cttatggagt ttttctagct tttaaggatt tccaaataga atgatcattc
     1201 cttgaataat aattctctat tttatcaata ttttgttttt ttttctattt tagaaagggc
     1261 ggagagtagt gtgtagtggc ggtttcacgc tctgatgatc tcatgccatt gttcaccttt
     1321 tccaaattgt tttaggttat tcggagtact tcttttcttt cttttccctc ttcattttca
     1381 ccctctgttt gagcatgagt gctctctatt ctttggtgat ggaataaaaa gaggtgccat
     1441 caaattcaga attttgtaaa atgttgaact ttagagtttt tagtattcgg ttagtgtgat
     1501 gtttgtttta ttttcaaccc taacaaatct cagttttctg gaaaagttgt gcatttacaa
     1561 tgaatatatt gttttctgca tttcgtctgt tcttagatta ttcagtgact ttggaaattt
     1621 aagctaattc ttgttgagcc atttgacata atttgtatag aaaacaaaaa tgtatacttt
     1681 acaccgcgat ttagatacgg cactgtgcgg aaactagcaa acaattcaga gccgacttcc
     1741 ataaggttgc caaaaaaatt ttagtaattt attaattcaa taattaaatg tatttatcac
     1801 gaaaataaaa tgaaaacatt tgttgttcaa aattatatat tttcagaaag aaaaaacaag
     1861 tttttaaaat ttaattacta cttctcatta tgagtctctc aaaaagctca acatttcagt
     1921 tagaataaaa attaaaaaat atcaatgaaa gtgtcccgtc taattccatt tttcactccc
     1981 cacactgttc aacgtcaaaa cacattgtca ttcagtcatt ttggaaaaag caccccgagc
     2041 gcagaagcag taggaaaaac gagtctcgat gggggtaggt tgaacgagca aaaaaaacca
     2101 attcggctaa ttgaactcga ctttttctcc caattttact ctcaccaatc gtgatgcgta
     2161 tctaaatgga tacgcaaaga aaggaaaaga aagagctttt tattttcaga tccacccacg
     2221 gacacgtttt tggtagaact attacatcac tttttcttag ttttttctgt gagcttgatg
     2281 agaactgtgg tgtatttttt gtagataaaa caaaatttaa acctacttca taagttcaca
     2341 cacctacctt atgtcagcct agaaagttac cacgatgatg gcagaaagtt gcctctttgc
     2401 ctctaaatgc cgagtagttt caaaaaatgt tgacatttga tttggaattt aagagtcctc
     2461 tctgaaaatg tgtaattctg aaattttagt gcccatttca aaaaaataac atctgctcag
     2521 caaattgtat ttcatcctct ctttattttt tgttaccccg tcttacttcc tctctttttt
     2581 atttattgtt ttttcttgtg gtcctaataa taaaaatgaa aagagaagct gttgtgctct
     2641 tccacaacaa tgtgctcttt tcaatattct aaaatggcaa tggaattggc aattgttgga
     2701 gggttaaaat tgccagacaa aagtcatctt ctctatgttt gttcttcatc ttcttttacc
     2761 tctattcctt atccttctca ataacatcct gaaactgacg atttaatgag tatacttccg
     2821 tttatgaatg tatcatgata atttcatgtc tgaaaaagaa cggagaaatg agaatgcata
     2881 ttctcaactt gaacgtatag ccaagactta tgcctatttt caatctgttt agaccctgac
     2941 aaacatttga ccacacagga gtgtttggga caacaaccat atttttgaaa acatttttga
     3001 atgagaaatg ctatcttctg tgggaagttt taaagcaagc tgatttcttt atatagccca
     3061 aaataattat tttttaatag attatgcaaa ttaaaatatg gagcttgatt tgctgaagtg
     3121 tctatttgat ttgatttgat ttgatttgat ttattacgat tggaatcgtg atggaataaa
     3181 agcagtatag tataaagaca gaaaaaataa atacacaata cataggaaga ataatcaaaa
     3241 agttacgaat aaagtacaaa caagaaaagc aattatcacc tgcaaaagtt gattaattgg
     3301 aacattaaag tgaacatgaa ataggctatt tgggggtgtg aggagagtgg gtgaatgatg
     3361 ggtaataaga ggtgccaata taagattcgt catgctaatt tttcatagaa ctttagaccc
     3421 tatttgtgaa aaagtgtctt cttagtttgg ttttgcattt cccccagatg aacttggttt
     3481 tggttctggt tttgttgttt ttatcgattt tgaaaaattg gtccgtgtca atgtcaactt
     3541 tatttaactt taagctttaa agaataaact tatcaaaata aatcaaacgg tacttttttt
     3601 ttgaaatttt tattcaaaat tcaatctttt caagagcaaa ctgtaatttt ttttgtcatt
     3661 cctatcgcaa ataaacgtaa aattcagtta atttaaaatg tgagaaatga ggtaacaact
     3721 actatagaaa aataatgaaa tgttcaagcg atgagcaatt aaaatttacg ctcaaaaatt
     3781 aaccatgatg agaaaaaaat tctgctcttc aagttataca agacttatga gtgttaactt
     3841 tatacttcaa aaaataaatt gtattgaaac acctttttta gagattttag attattagat
     3901 tgagaactgt acaaaaaaac gagtgttcca agatacatga gaatgatatt ttaacccgaa
     3961 acaatgaaac attcaatcat acatattctc atccttttca actcgtcaac attccacgcc
     4021 ttgtctcact tttttctatt tcccgtccac tgggattttg atttcccgcc cgtttcgtct
     4081 cggcattttt tttttgaaag aggttactct ctcatctttg acaccatctt ccataaaaca
     4141 cgagaaatga ataatggaat cgagggaggt tcttgtttgt tttttttgtg tgtttttgtt
     4201 ttgcatttta ctcatatttt agaacttcga aatctgatca tgactcgttt tgcgagaacc
     4261 attgctttaa aatattttta ttttcttgag gataactgtt tttttgaaaa aaaaaacatg
     4321 gaatctttgg aagtatgctg taaattgagg aagtcttaaa atgtttcaac ttccatatga
     4381 aaaattaaat ataatacagt tttgtttccg ttacaaaatc caagacgtct tctcacaaaa
     4441 aatcgcctaa attttcattt aaaaatgtgt ctttgcaaaa gttaccagtc ctgtcaaata
     4501 ctcgcaacaa atatccaaga ataactgact gatctgattt ttgcagttag tttttcctct
     4561 tcaaactcaa aatctgtttt tatttgaacc attcaatagt ttaaacccca tttgaatacg
     4621 ttttctcatc tttcctcgtc accagtttca atgctttaaa aacaattttc agcatcccgg
     4681 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
     4741 caggaggacc cttggctagc gtcgacggat cccgatgtcg gacacaattc ttggtttcaa
     4801 caaatcaaac gtcgttttgg ctgctggaat tgctggagcc gctttcctcg gctactgcat
     4861 ttacttcgat cataagagaa tcaacgctcc agactacaag gacaagatta ggcaaagtca
     4921 gtgttttaac aacatatttc cttcggattt ttatctaaaa acaacttatt ttctttcaga
     4981 gagacgtgcc caggctgggg atcctatgga gaaaagtttc gtgataactg atccacggct
     5041 gccagacaat cccatcatct tcgcatccga tggcttcctg gagctgaccg agtattccag
     5101 agaggagatc ctgggccgca atggccgctt tctgcaggga ccagagacag accaggccac
     5161 agtgcagaag attcgcgatg ccattagaga tcagcgcgag attaccgtgc agctgataaa
     5221 ctacacaaaa agcgggaaga aattctggaa cctcctgcac ctccagccca tgagggacca
     5281 gaagggtgag ctccagtatt tcatcggagt gcagctggat ggataaagat ccccgagatc
     5341 ttcagcgctc agcattcgta gaattgtaag tttaaacttt ctcatactaa ctaacgcgct
     5401 ctatttaaat tttcagaatt cgctgtctca tcctactttc acctagttaa ctgcttgtct
     5461 taaaatctat gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat
     5521 ggtctcttct caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt
     5581 ttttgcgagt ttatatacct tccaattttc tttctattgt attttcaact tctaatttta
     5641 attcagggaa actgcttcaa cgcatcggta ccatggtctc caagggagag gagctcttca
     5701 ccggagtcgt cccaatcctc gtcgagctcg acggagacgt caacggacac aagttctccg
     5761 tccgtggaga gggagaggga gacgccacca acggaaagct caccctcaag ttcatctgca
     5821 ccaccggaaa gctcccagtc ccatggccaa ccctcgtcac caccctctcc cacggagtcc
     5881 agtgcttcgc ccgttaccca gaccacatga agcagcacga cttcttcaag tccgccatgc
     5941 cagagggata cgtccaggag cgtaccatct tcttcaagga cgacggaacc tacaagaccc
     6001 gtgccgaggt caagttcgag ggagacaccc tcgtcaaccg tatcgagctc aagggagtcg
     6061 acttcaagga ggacggaaac atcctcggac acaagctcga gtacaacttc aactcccaca
     6121 acatctacat catggccgtc aagcagaaga acggaatcaa ggtcaacttc aagatccgtc
     6181 acaacgtcga ggacggatcc gtccagctcg ccgaccacta ccagcagaac accccaatcg
     6241 gagacggacc agtcctcctc ccagactccc actacctctc cacccagtcc gtcctctcca
     6301 aggacccaaa cgagaagcgt gaccacatgg tcctcctcga gttccgtacc gccgccggaa
     6361 tcaccctcgg aatggacgag ctctacaagt gataagatat cacctcccac aacgaggatt
     6421 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     6481 aattgtataa gtaggagctc ccgagatctt cagcgctcag cattcgtaga attgtaagtt
     6541 taaactttct catactaact aacgcgctct atttaaattt tcagaattcc aactgagcgc
     6601 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga
     6661 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg
     6721 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     6781 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     6841 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     6901 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     6961 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     7021 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     7081 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     7141 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     7201 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     7261 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     7321 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     7381 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     7441 ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     7501 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7561 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     7621 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7681 gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta
     7741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7861 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7921 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7981 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8041 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8101 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8161 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8221 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8281 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8341 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8401 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8461 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8521 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8581 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8641 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8701 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8761 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8821 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8881 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9721 cgcaaac
//
