LOCUS       sequence-355668-        6270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans 
            AVB/others premotor IN and others expression of miniSOG-SL2 BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3453
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1249..1569
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(3742..3764)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3864..3883
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3961..3979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4047..4151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4152..4220,4221..5012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4370..4389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5183..5771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5672..5691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5925..5942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6059..6080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6095..6100,6101..6118,6119..6125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6133..6149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6138..6160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6157..6173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6157..6173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgatggatac gctaacaact tggaaatgaa ataagcttgg gctgcagcat gcggccgctg
       61 ttgcttttct tctcctcacc ccccgtcgtc tcttcttttt tactattccc cacgagaaac
      121 tgcccaccac aaaaacttac tttccagaaa aatattggca aatagatgtc gaaatgaaat
      181 ggctagcttt gatgatggta agcgtttttt tttttctaca aaattgtgga ttaagaatct
      241 tccaaaaccg ttatactaca ctatatccaa atataaaaaa attttggccc aggcgggggg
      301 tcgaaccagg gccttcagat tggtagacca ccccgttacc gcctgagcca ctttttccca
      361 tttttgaagt tgatggctaa gattggagtt tgaacgattg aacgatttta gagcaaaatt
      421 ttatggggat tctgaaaata tagtttattt taagtttgga gcatttttta tgggctcaaa
      481 atgactcgga agctcgaaaa tctgaaattt tcaaaacttt taatgtgggc ttttcaaatt
      541 tcaaatttcc gtgaaaaata ttgaatattg gttgtaaatt ttaagaaata tatgaatttc
      601 aacactttat tatacgaaaa actgcatgca aaaattttca ggtcaaatca tgtgcaaatt
      661 ttgtgctgat ttcgaatttt tatatcattt tgtaccaaaa cccctagaat tttagaaact
      721 tttgaacttc tgttaagtaa tgactcactt ttcgagcaaa atcgaatgtt ccaaaaatag
      781 gcaaaacatc tgcccgcgca ttaagctcaa ccacgacaat tttattggac aggaagttgt
      841 ttggtgacta attgaatttc aattccccgt cccccctctc cacacaaata ccactacgaa
      901 catctctttt gacggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
      961 actaacataa catagaacat tttcaggagg acccttggct agcgtcgacg gatcccgatg
     1021 tcggacacaa ttcttggttt caacaaatca aacgtcgttt tggctgctgg aattgctgga
     1081 gccgctttcc tcggctactg catttacttc gatcataaga gaatcaacgc tccagactac
     1141 aaggacaaga ttaggcaaag tcagtgtttt aacaacatat ttccttcgga tttttatcta
     1201 aaaacaactt attttctttc agagagacgt gcccaggctg gggatcctat ggagaaaagt
     1261 ttcgtgataa ctgatccacg gctgccagac aatcccatca tcttcgcatc cgatggcttc
     1321 ctggagctga ccgagtattc cagagaggag atcctgggcc gcaatggccg ctttctgcag
     1381 ggaccagaga cagaccaggc cacagtgcag aagattcgcg atgccattag agatcagcgc
     1441 gagattaccg tgcagctgat aaactacaca aaaagcggga agaaattctg gaacctcctg
     1501 cacctccagc ccatgaggga ccagaagggt gagctccagt atttcatcgg agtgcagctg
     1561 gatggataaa gatccccgag atcttcagcg ctcagcattc gtagaattgt aagtttaaac
     1621 tttctcatac taactaacgc gctctattta aattttcaga attcgctgtc tcatcctact
     1681 ttcacctagt taactgcttg tcttaaaatc tatgcttctc tttagtatct aaaattttcc
     1741 tagaagctta caagtatata aatggtctct tctcaataaa ggttgtatat ttattcatct
     1801 tattgaatct gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat
     1861 tgtattttca acttctaatt ttaattcagg gaaactgctt caacgcatcg gtaccatggt
     1921 ctccaaggga gaggagctct tcaccggagt cgtcccaatc ctcgtcgagc tcgacggaga
     1981 cgtcaacgga cacaagttct ccgtccgtgg agagggagag ggagacgcca ccaacggaaa
     2041 gctcaccctc aagttcatct gcaccaccgg aaagctccca gtcccatggc caaccctcgt
     2101 caccaccctc tcccacggag tccagtgctt cgcccgttac ccagaccaca tgaagcagca
     2161 cgacttcttc aagtccgcca tgccagaggg atacgtccag gagcgtacca tcttcttcaa
     2221 ggacgacgga acctacaaga cccgtgccga ggtcaagttc gagggagaca ccctcgtcaa
     2281 ccgtatcgag ctcaagggag tcgacttcaa ggaggacgga aacatcctcg gacacaagct
     2341 cgagtacaac ttcaactccc acaacatcta catcatggcc gtcaagcaga agaacggaat
     2401 caaggtcaac ttcaagatcc gtcacaacgt cgaggacgga tccgtccagc tcgccgacca
     2461 ctaccagcag aacaccccaa tcggagacgg accagtcctc ctcccagact cccactacct
     2521 ctccacccag tccgtcctct ccaaggaccc aaacgagaag cgtgaccaca tggtcctcct
     2581 cgagttccgt accgccgccg gaatcaccct cggaatggac gagctctaca agtgataaga
     2641 tatcacctcc cacaacgagg attacactat cgttgagcaa tatgaaagag ctgaagggcg
     2701 gcactcgaca ggtggcatgg atgaattgta taagtaggag ctcccgagat cttcagcgct
     2761 cagcattcgt agaattgtaa gtttaaactt tctcatacta actaacgcgc tctatttaaa
     2821 ttttcagaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     2881 taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat
     2941 atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     3001 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     3061 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     3121 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     3181 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     3241 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     3301 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     3361 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     3421 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     3481 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     3541 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     3601 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     3661 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
     3721 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3781 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     3841 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3901 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     3961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4381 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6121 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6181 acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca
     6241 gagcttaaaa atggctgaaa tcactcacaa
//
