LOCUS       sequence-35567-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pLR-NN
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..98
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     90..107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     91..107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
       61 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      121 ccgtctcgct atcgccagca acaatggcgg caagcaagcg ctcgaaagca ttgtggccca
      181 gctgagccgg cctgatccgg cgttggccgc gttgaccaac gagagacgtc tagaccagcc
      241 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
      301 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
      361 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      421 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
      481 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      541 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
      601 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      661 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
      721 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      781 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
      841 ctgtagaagt caccattgtt gtgcacgacg acatc
//
