LOCUS       sequence-355687-        8161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmBicaudalC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8161)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmBicaudalC_P
FEATURES             Location/Qualifiers
     source          1..8161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4383..4392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5467..5508
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5518..5535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5558..5575)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5608..5742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5632..5651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5686..5705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6343..6931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6423..6442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7102..7893,7894..7962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7725..7744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7963..8067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8135..8153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgatgttgt cctgtgcctc tttcaataaa ctgatgtatc cgtcggccgc
     2761 agatgttgcc aaaccgccaa tggtgggctt ggaggtggaa gctggatcca ttggatccct
     2821 gtccagtctt cacgcccttc catcgaccac ctcggtgggc agtggagccc ccagcgagac
     2881 gcagagcgag atctcctccg tggatagcga ttggagtgac atacgcgcca tcgccatgaa
     2941 gttgggcgtt caaaacccag acgatctcca cacggaacgt ttcaaggtgg atcgacagaa
     3001 attggagcag ttaataaaag cggagagctc cattgaggga atgaatggag ctgaatattt
     3061 tttccatgac atcatgaaca ctacggatac ttatgtgagc tggccttgca gactcaaaat
     3121 tggtgccaag agcaagaagg atccccatgt gcgaattgtg ggaaaggtgg atcaggttca
     3181 gcgggcaaag gagcgcatcc ttagcagtct tgactccagg ggcactcgag tgatcatgaa
     3241 aatggacgtc agctacacgg atcactcgta catcatcgga cgaggtggca acaacatcaa
     3301 gcgcataatg gacgacacgc atacccatat ccacttcccc gactcgaatc gctccaatcc
     3361 cacggagaaa tccaaccagg tgtcgctgtg cggcagccta gagggcgtgg agcgagcccg
     3421 ggctctcgtt cggctctcca caccgctgct catctccttc gagatgcccg taatgggacc
     3481 taacaagccg cagcccgacc acgagactcc ctacatcaag atgatcgaga ccaagttcaa
     3541 tgttcaggtc atattctcca cgcgtccaaa attgcatacc tcgctggttt tggtcaaggg
     3601 atccgagaag gaatcggcgc aggtccgaga tgccacgcag ctgctgatca acttcgcctg
     3661 tgagagtatt gcgagccaaa tcctggtgaa tgtccagatg gagacctcgc cgcagcatca
     3721 cgaaatcgtc aagggcaaga acaacgtgaa tctgctgagc attatggagc gaacccagac
     3781 caagatcata ttccccgatc tgagcgacat gaacgtgaag cccctgaaga agtcccaggt
     3841 cacgatcagc ggacgcatcg acgacgtcta cttggcgcga caacaattgc ttggcaattt
     3901 gcccgtagca ttgatttttg actttccgga caaccacaac gatgcatccg aaataatgag
     3961 cctcaacacc aagtacggcg tctacatcac gttgcgccag aaacagcggc agagcaccct
     4021 ggccattgtg gtcaagggag tggagaagtt tatagacaaa atctacgagg cccgccagga
     4081 gatcctgcgc ctggccacac cattcgtgaa gcccgagatc cccgactact acttcatgcc
     4141 aaaggacaaa gaccttaatc tcgcctaccg cacccagcta actgctctgc tggctgggta
     4201 tgtggacagc ccgaagactc catcgctgtt gccgccctcc ttggctggcc aactgacgcc
     4261 ctatgccaac aacaatcacc tgttgttgaa cgccaatggc ttggccacgc ccactggggt
     4321 gtgtgcgccc acccaaaaat atatgcaatt gcacaacagc ttccagcaga ctcagaatcg
     4381 ttccatggtg gctggtgggc agagcaacaa tggcaactat ctgcaggttc ccggagcggt
     4441 ggcgccgcct ctcaaaccgc cgactgtttc gccacggaac agctgcagtc agaataccag
     4501 tggttaccag agctttagca gcagcaccac ctccctggag cagtcgtatc cgccgtatgc
     4561 ccaattgccg ggcaccgtgt cgtccacttc atcctccacc gcgggaagcc aaaatcgggc
     4621 gcattattcg ccagattcca cgtacggcag tgaaggtggt ggcgttggcg gcggaggtgg
     4681 tggtggggcg cgtctgggac gacgccttag cgatggagtg ctgctggggc tgggtaattc
     4741 caacggtgga ggaggtaact ccggaggagc ccacctcctg cccggcagtg ccgagagcta
     4801 tcggagtttg cactatgacc ttggaggaaa caagcactcc ggccaccgtg cctttgactt
     4861 cgacatgaaa cgggccctgg gctacaaggc catggagcgc accccggtgg caggggagct
     4921 gcgaacaccc acgaccgctt ggatgggaat gggcttgagc agcacctcgc ccgcccctgc
     4981 cccgctggag aacggggaaa atggggctgc tggtggagga gcatctagtg ggtggcggct
     5041 gccaccagga ctgggttccc cttacggact gagtgccacc accggactcc tggacgccac
     5101 tccagtcaac cgacggatgc agctagccaa gcacaaggac atccagactc tgctcaccag
     5161 tttgggtctg gagcactaca tcaaaatatt cgttctaaac gaaatcgacc tggaggtgtt
     5221 caccaccctg accgaggaaa acctgatgga gctgggtatc gcggcgttcg gagctcgcaa
     5281 gaagctgctg acggccattc acacgctgct ggccaacgag gcggcctgca gcacaatgcc
     5341 cagcagcagc tcctcgcaga actcctcgtc gccgcgattc tccggaagtg cggccccagg
     5401 cgcagagcgc cgtccctcca accagtggtg agcggccgct cgagtctaga gggcccgcgg
     5461 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     5521 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc
     5581 tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt
     5641 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     5701 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     5761 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     5821 tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc
     5881 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     5941 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     6001 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     6061 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     6121 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     6181 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     6241 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca
     6301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6781 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7381 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     7441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     7741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8101 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8161 t
//
