LOCUS       sequence-355689-        9648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Syt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYT7alpha
FEATURES             Location/Qualifiers
     source          1..9648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2556..3002
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4294..4882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4347..4367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4765..4776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4885..4901)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4886..4902)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5407..5587
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5613..5630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5619..5843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5889..6317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5976..5995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6186..6207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6326..6346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6331..6660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6511..6646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6573..6592
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6708..6755
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6774..7148
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7278..7399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7315..7334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7369..7388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7448..7464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7448..7464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7461..7483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7472..7488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7496..7502,7503..7520,7521..7526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7541..7562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7679..7696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7850..8438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7930..7949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8609..9400,9401..9469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9232..9251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9470..9574)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaaagtgc aagtggtttt aggaccagga
     2581 tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa
     2641 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
     2701 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
     2761 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgt
     2821 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
     2881 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
     2941 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
     3001 aggtacggct agcggatccg gtaccgcggg cccgccacca tgtaccggga cccggaggcg
     3061 gccagcccag gggcacctac ccgcgatgtc ctgctggtct ctgcaatcat caccgtcagc
     3121 cttagcgtca ctatcgtcct ctgcggcctg tgccactggt gtcagcgcaa actgggcaaa
     3181 cgctacaaga attccttgga gacggtgggc acgccagact cggggcgtgg gcgcggtgag
     3241 aagaaagcca tcaagttgcc tgcaggaggg aaggctgtga atacagcccc agtgcccggc
     3301 cagacgccac acgatgagtc tgaccgcaga acggagaccc gttcctctgt ctcggacctc
     3361 gtcaactccc ttaccagcga gatgctcatg ctctccccag gttctgagga ggatgaggcc
     3421 cacgagggct gcagccgaga gaacctgggc cgaatccagt tcagtgttgg ctacaacttc
     3481 caagagtcca cactcaccgt gaaggtcatg aaggcccaag agctgccagc caaggacttc
     3541 agcggtacta gtgacccctt tgtcaagatc tacctgctac ccgacaagaa gcacaaactg
     3601 gagaccaagg tgaagcggaa gaatctaaac ccgcactgga atgagacctt tctatttgaa
     3661 gggtttccct acgagaaagt ggtgcagagg gtcctctacc tccaggtcct ggattatgac
     3721 cgtttcagcc gcaatgaccc cattggggag gtgtccatcc ctctgaacaa ggtggacctg
     3781 acccagatgc agaccttctg gaaggatctg aagccatgca gcgatgggag tgggagccga
     3841 ggggagctgc tcttgtccct ctgctacaac ccctctgcca actccatcat cgtgaacatc
     3901 atcaaagctc gaaacctcaa agccatggac atcgggggca catcagaccc ctatgtgaag
     3961 gtgtggctga tgtataaaga caagcgggta gagaaaaaga agactgtgac aaagaagagg
     4021 aacctgaacc ccatcttcaa tgagtctttc gccttcgaca tacccacgga gaagctgagg
     4081 gagaccacga tcatcatcac tgtcatggac aaagacaagc tcagccgcaa cgacgtcatc
     4141 ggcaagatct acctgtcctg gaagagcgga ccaggtgaag tgaaacactg gaaggacatg
     4201 atcgctcgtc cccggcagcc tgtggcccag tggcaccagc tgaaagcctg aggccgcact
     4261 cctcagctag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     4321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4381 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4441 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4501 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4561 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4621 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4681 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4741 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4801 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4861 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     4921 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4981 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5041 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5101 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5161 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5221 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     5281 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     5341 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     5401 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5461 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5521 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5581 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5641 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5701 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5761 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5821 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5881 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5941 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6181 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6241 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6301 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6361 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6481 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6541 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6601 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6661 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     6721 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     6781 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     6841 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     6901 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     6961 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     7021 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     7081 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     7141 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7201 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7261 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7381 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7441 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7501 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7621 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7681 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8281 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8401 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8521 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8581 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8641 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8701 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8761 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8821 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8881 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8941 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9001 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9061 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9121 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9181 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9241 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9301 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9361 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9421 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9481 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9541 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9601 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcag
//
