LOCUS       sequence-35569-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNN9
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     784..803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
       61 aaatatatca tcatgcctcc tctagaggtc tcctcgaaac ggtgcagcgg ctgttgccgg
      121 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg
      181 gcggcaagca agcgctcgga gaccctcgag ccacccatga ccaaaatccc ttaacgtgag
      241 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      841 tcgatttttg tgatgctcgt
//
