LOCUS       sequence-355691-       10689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Syt7 with N terminal HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn PP-HaloTag-SYT7alpha
FEATURES             Location/Qualifiers
     source          1..10689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..119
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             144..1028
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDPIG
                     SEIARWLSTLEISG"
     misc_feature    2325..2913
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2378..2398)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2796..2807)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2916..2932)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2917..2933)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3438..3618
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3644..3661)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3650..3874
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3920..4348
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4007..4026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4217..4238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4357..4377)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4362..4691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4542..4677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4604..4623
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4739..4786
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4805..5179
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5309..5430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5346..5365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5400..5419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5479..5495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5479..5495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5492..5514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5503..5519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5527..5533,5534..5551,5552..5557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5572..5593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5710..5727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5881..6469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5961..5980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6640..7431,7432..7500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7263..7282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7501..7605)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7680..7699)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7871..8250
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8252..8450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8405..8425
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8468..8648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8695..8820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9313..9546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9731..9775
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10073..10190
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10235..10681
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 agcggatccg gtaccgcggg cccgccacca tggacagcaa aggttcgtcg cagaaagggt
       61 cccgcctgct cctgctgctg gtggtgtcaa atctactctt gtgccagggt gtggtctccg
      121 atatcggtac cacgcgtgga tccgaaatcg gtactggctt tccattcgac ccccattatg
      181 tggaagtcct gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg
      241 tgctgttcct gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg
      301 ttgcaccgac ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac
      361 cagacctggg ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc
      421 tgggtctgga agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact
      481 gggccaagcg caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta
      541 tcccgacctg ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca
      601 ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga
      661 tgggtgtcgt ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga
      721 atcctgttga ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc
      781 cagcgaacat cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc
      841 cgaagctgct gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc
      901 tggccaaaag cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc
      961 aagaagacaa cccggacccg atcggcagcg agatcgcgcg ctggctgtct actctggaga
     1021 tttccggtgg atccggaagt tcggggtcca gcggttcttc aggcagttcc atgtaccggg
     1081 acccggaggc ggccagccca ggggcaccta cccgcgatgt cctgctggtc tctgcaatca
     1141 tcaccgtcag ccttagcgtc actatcgtcc tctgcggcct gtgccactgg tgtcagcgca
     1201 aactgggcaa acgctacaag aattccttgg agacggtggg cacgccagac tcggggcgtg
     1261 ggcgcggtga gaagaaagcc atcaagttgc ctgcaggagg gaaggctgtg aatacagccc
     1321 cagtgcccgg ccagacgcca catgatgagt ctgaccgcag aacggagacc cgttcctctg
     1381 tctcggacct cgtcaactcc cttaccagcg agatgctcat gctctcccca ggttctgagg
     1441 aggatgaggc ccacgagggc tgcagccgag agaacctggg ccgaatccag ttcagtgttg
     1501 gctacaactt ccaagagtcc acactcaccg tgaaggtcat gaaggcccaa gagctgccag
     1561 ccaaggactt cagcggtact agtgacccct ttgtcaagat ctacctgcta cccgacaaga
     1621 agcacaaact ggagaccaag gtgaagcgga agaatctaaa cccacactgg aatgagacct
     1681 ttctatttga agggtttccc tacgagaaag tggtgcagag ggtcctctac ctccaggtcc
     1741 tggattatga ccgtttcagc cgcaatgacc ccattgggga ggtgtccatc cctctgaaca
     1801 aggtggacct gacccagatg cagaccttct ggaaggatct gaagccatgc agcgatggga
     1861 gtgggagccg aggggagctg ctcttgtccc tctgctacaa cccctctgcc aactccatca
     1921 tcgtgaacat catcaaagct cgaaacctca aagccatgga catcgggggc acatcagacc
     1981 cctatgtgaa ggtgtggctg atgtataaag acaagcgggt agagaaaaag aagactgtga
     2041 caaagaagag gaacctgaac cccatcttca atgagtcttt cgccttcgac atacccacgg
     2101 agaagctgag ggagaccacg atcatcatca ctgtcatgga caaagacaag ctcagccgca
     2161 acgacgtcat cggcaagatc tacctgtcct ggaagagcgg accaggtgaa gtgaaacact
     2221 ggaaggacat gatcgctcgt ccccggcagc ctgtggccca gtggcaccag ctgaaagcct
     2281 gaggccgcac tcctcagcta gaattcgata tcaagcttat cgataatcaa cctctggatt
     2341 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2401 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2461 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2521 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2581 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2641 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2701 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     2761 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2821 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     2881 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     2941 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     3001 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     3061 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3121 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     3181 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     3241 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     3301 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     3361 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     3421 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3601 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     3661 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3721 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3781 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3841 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3901 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4321 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4441 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4501 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4621 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4681 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     4741 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     4801 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     4861 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     4921 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     4981 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     5041 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     5101 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     5161 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     5221 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5281 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5401 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5461 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7621 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     7681 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     7741 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7801 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     7861 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     7921 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     7981 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8041 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8101 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8161 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8221 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     8281 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     8341 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     8401 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     8461 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8521 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8581 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8641 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8701 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8761 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8821 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8881 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8941 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9001 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9061 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9121 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9181 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9241 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9301 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9361 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9421 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9481 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9541 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9601 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9661 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9721 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9781 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9841 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9901 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     9961 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    10021 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
    10081 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10141 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    10201 acagggacag cagagatcca gtttggttaa ttaaagtgca agtggtttta ggaccaggat
    10261 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
    10321 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
    10381 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
    10441 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgta
    10501 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
    10561 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
    10621 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
    10681 ggtacggct
//
