LOCUS       sequence-355693-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Syt7 with C terminal HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYT7alpha-HaloTag
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4337..4357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    5303..5891
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5356..5376)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5774..5785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5894..5910)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5895..5911)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6416..6596
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6622..6639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6628..6852
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6898..7326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6985..7004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7195..7216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7335..7355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7340..7669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7520..7655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7582..7601
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7717..7764
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7783..8157
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8287..8408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8324..8343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8378..8397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8457..8473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8457..8473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8470..8492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8481..8497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8505..8511,8512..8529,8530..8535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8550..8571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8688..8705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8859..9447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8939..8958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9618..10409,10410..10478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10241..10260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10479..10583)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatgta ccgggacccg gaggcggcca gcccaggggc
     3121 acctacccgc gatgtcctgc tggtctctgc aatcatcacc gtcagcctta gcgtcactat
     3181 cgtcctctgc ggcctgtgcc actggtgtca gcgcaaactg ggcaaacgct acaagaattc
     3241 cttggagacg gtgggcacgc cagactcggg gcgtgggcgc ggtgagaaga aagccatcaa
     3301 gttgcctgca ggagggaagg ctgtgaatac agccccagtg cccggccaga cgccacacga
     3361 tgagtctgac cgcagaacgg agacccgttc ctctgtctcg gacctcgtca actcccttac
     3421 cagcgagatg ctcatgctct ccccaggttc tgaggaggat gaggcccacg agggctgcag
     3481 ccgagagaac ctgggccgaa tccagttcag tgttggctac aacttccaag agtccacact
     3541 caccgtgaag gtcatgaagg cccaagagct gccagccaag gacttcagcg gtactagtga
     3601 cccctttgtc aagatctacc tgctacccga caagaagcac aaactggaga ccaaggtgaa
     3661 gcggaagaat ctaaacccgc actggaatga gacctttcta tttgaagggt ttccctacga
     3721 gaaagtggtg cagagggtcc tctacctcca ggtcctggat tatgaccgtt tcagccgcaa
     3781 tgaccccatt ggggaggtgt ccatccctct gaacaaggtg gacctgaccc agatgcagac
     3841 cttctggaag gatctgaagc catgcagcga tgggagtggg agccgagggg agctgctctt
     3901 gtccctctgc tacaacccct ctgccaactc catcatcgtg aacatcatca aagctcgaaa
     3961 cctcaaagcc atggacatcg ggggcacatc agacccctat gtgaaggtgt ggctgatgta
     4021 taaagacaag cgggtagaga aaaagaagac tgtgacaaag aagaggaacc tgaaccccat
     4081 cttcaatgag tctttcgcct tcgacatacc cacggagaag ctgagggaga ccacgatcat
     4141 catcactgtc atggacaaag acaagctcag ccgcaacgac gtcatcggca agatctacct
     4201 gtcctggaag agcggaccag gtgaagtgaa acactggaag gacatgatcg ctcgtccccg
     4261 gcagcctgtg gcccagtggc accagctgaa agccggatcc ggaagttcgg ggtccagcgg
     4321 ttcttcaggc agttccgagg atctgtactt tcagagcgat aacgatggat ccgaaatcgg
     4381 tattggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     4441 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     4501 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     4561 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     4621 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     4681 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     4741 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     4801 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     4861 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     4921 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     4981 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     5041 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     5101 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     5161 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     5221 gatcgcgcgc tggctgtcta ctctggagat ttccggttaa ggccgcactc ctcagctaga
     5281 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     5341 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5401 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5461 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5521 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5581 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5641 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5701 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5761 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5821 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5881 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     5941 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6001 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6061 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6121 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6181 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6241 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6301 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6361 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     6601 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6661 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6721 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6781 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6841 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7081 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7201 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7321 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7621 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7681 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7741 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7801 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7861 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7921 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     7981 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8041 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8101 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8161 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8221 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8281 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8401 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8461 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8521 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8581 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8641 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8701 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8761 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8821 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8881 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8941 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9001 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9061 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9121 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9181 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9241 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9301 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9361 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9421 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9481 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9541 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9601 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9661 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9721 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9781 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9841 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9901 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9961 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10021 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10081 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10141 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10201 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10261 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10321 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10381 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10441 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10501 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10561 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
