LOCUS       sequence-355714-        8531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D 
            premotor IN and others expression of miniSOG-SL2 BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3456
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3816..4136
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(6309..6331)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6431..6450
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6528..6546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6614..6718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6719..6787,6788..7579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6937..6956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7750..8338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8239..8258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8492..8509
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
      241 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
      301 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgggctg cagcatgcgg
      361 ccgctgtggt ctcactttga acgacaaaag aaaaccaaat attgtaaagg aatagtacag
      421 ataaaaggga acaagtgata ccagctgatg gatgtaggtc agcgacaaaa gacaggagac
      481 gtatagtttg atgattatgg aaccaaactc agaatttaat gttggagacg ctataaaaat
      541 tgaattgaga aattgaaacc atattcttga atatctctca atttggaatt tggaagtagc
      601 cgtaaaatcg gctagctaga acataaaagg actgatttgc aaccttgacc attcatgata
      661 tcatttcaat aaaagaagag attccaatga ccaatcttca agaatcacaa taatcacaac
      721 aaaactaagc aaaagtaaca acaaattgtt caggtagaca agtagacatt tggacacatt
      781 ttgcggggat gtgatgactg tataataaaa ataagtggac actgagagag agaggaggtc
      841 agcaggaaaa aagaacatcg aactgcttga cgaattgaaa ttagaatgag gtgtcaggca
      901 ggtgacgatt acatggttaa ttattagtgt tctttttctt ctttttgtgg atgccgatta
      961 gttgaaataa ttgcatggaa agtgagaatt caatgtgaat ctctttctta ttatgtttca
     1021 agtggtatct ggatggaagc ttgtactttt atgaatcgaa aattgcgccc aaggttgatt
     1081 ttgagattat tcagtaaatt tttgtgctga ttattcagtt aatgtagaac agagacgcgg
     1141 atgaaaactt ttttaatttc taaaaaaaaa gacaaaatgt ggttaaaaag tttggaaaat
     1201 aattgttata tacttcttga gagcttattc aaacggaaac caatcataaa tagtcgaata
     1261 gtcatttaga aaagagaaac tagttgatgt agagagattc aaaaagtaaa atatgcaaat
     1321 gatccgtttc ttagatttag taaacatcta ccaaatgaaa caggaattgt cgaaacaatt
     1381 tagaaaagga gaaggaaaca tttgtttatt ttccccttta aatggcggta gaggtaaaac
     1441 agcaatacac atagtacaag gttatactac tttcaaaaga atttgacatc ccgccgagaa
     1501 aatgtggtga ttatatggta aagttttcag ttggatatta tcttctacag acggtgattc
     1561 aaaatgatca aaagggcaag tgggtggtag gatttgaaaa attgagatat agaacgtgcg
     1621 attttggtaa aactttttga acacaaggca tatttttgaa ctttgaaaaa cgcaaaattt
     1681 aaattttgtt aattacatat attttgaaca gattatgaat ataagcaagg ctacataaaa
     1741 tatgtatatt ttctttttac aaatattcgt acactgttaa atttatagaa aaatcattta
     1801 tattcagaaa aacctatttg aacaattttt agttgctcaa taaccaattt gcacaaattt
     1861 agaaaatata atgggggctt ttaaaaattt aaatcctcag cgaatattcc tttgcgattt
     1921 tttcgaacgt tttctttcag attaaaaaat ctgaagcaga agtaaatgaa ctaccataga
     1981 aaattcacca aggatcacat ttttacaaag cctccgccca attgtaaact tgggaatttt
     2041 tgcttgtttt cttttttttt tcaaaaaagg aatagttgtt ttaaagtaat ttattcccct
     2101 atgctttctc cagcaatcag ttgttctccc catactttac acaattatca acaacttcac
     2161 atagaaaaca aaacaacaaa tcagaaattc ccaccggtca cagtcacaga ttccccatgt
     2221 tttgttcatc tcatttctgc aagaaagtga catcgctgcc acccaattcg acaaattgtc
     2281 caagtggtca tcaggaagat atgaccttct ttttaatgat aatttagact acaaagggcg
     2341 tcaaaaaaga ttcaatgatc aggggtaatt gctgtaaaat gtagtttgta tgtgtgttta
     2401 ccgtagccta taactgtaaa aattggtttt ttaaaaagta tttctgagat taaaaggctc
     2461 cccaaggatg cacgattcta gctagaaatg aacattccat gggttgtgaa gatgctttta
     2521 tctcacttta gaatgtctga ctccgcccct ctgtgtacga attgaagtgc tcttttgtga
     2581 tgatcatagc tcctccttgc ggcgacgccc gcacacagca cgagatatta cacggaatac
     2641 ttatgtgatt cataggaaac aaggaaatcg aaaaaataat aggattttga ggaggtattt
     2701 ttagatgaat gcttatgatg ttatgatgaa cccaagaaat tctgaataga catatgtcag
     2761 aacggggtag gagctccagt gtcgcttttg aaataaaaaa tcaagtggta tacttctcgt
     2821 atataccctt ctgaactaat aaaataattt atcttttgaa aaagtttgaa aaagaaaaat
     2881 gcccaaaact tgaattttaa tgttttgtat tttgagcata tcgttcaaac gtcgtgatct
     2941 gaaagtagaa agatctaaat attcgtgaca cagtttggtt tgaaggtctt agtggtaatt
     3001 ttttattaca gaagcaacaa aaagttgaca tattttcaga atttccaaaa aaacgtttaa
     3061 aatttcaatt tcttcgatag agttctaaaa attttttgga acggattttg taatatccgc
     3121 tgataaccgt tcaagaacaa tttgaatttt gaaataattt aatcaactag tatccagtgc
     3181 tctttctcac tgctccaccc atttttcaac ccatttcctt tactcaattt cgcccgtttt
     3241 ccttttgctc aaatgtgccc accaaatcat ctttcttcgt cttcttttct cttattcgga
     3301 aggagaagag gtgaaagtag aataagtttt tttgttgaaa agagaggagc acggccactt
     3361 ttctcttatt tccatccttc tactacctcg ttttactttc catttaaaaa cccgtttaaa
     3421 gttccaaaat ttcaattatc ttgcttctgt ttggaacacg acaaacttta gttttgttac
     3481 agatacccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
     3541 agaacatttt caggaggacc cttggctagc gtcgacggat cccgatgtcg gacacaattc
     3601 ttggtttcaa caaatcaaac gtcgttttgg ctgctggaat tgctggagcc gctttcctcg
     3661 gctactgcat ttacttcgat cataagagaa tcaacgctcc agactacaag gacaagatta
     3721 ggcaaagtca gtgttttaac aacatatttc cttcggattt ttatctaaaa acaacttatt
     3781 ttctttcaga gagacgtgcc caggctgggg atcctatgga gaaaagtttc gtgataactg
     3841 atccacggct gccagacaat cccatcatct tcgcatccga tggcttcctg gagctgaccg
     3901 agtattccag agaggagatc ctgggccgca atggccgctt tctgcaggga ccagagacag
     3961 accaggccac agtgcagaag attcgcgatg ccattagaga tcagcgcgag attaccgtgc
     4021 agctgataaa ctacacaaaa agcgggaaga aattctggaa cctcctgcac ctccagccca
     4081 tgagggacca gaagggtgag ctccagtatt tcatcggagt gcagctggat ggataaagat
     4141 ccccgagatc ttcagcgctc agcattcgta gaattgtaag tttaaacttt ctcatactaa
     4201 ctaacgcgct ctatttaaat tttcagaatt cgctgtctca tcctactttc acctagttaa
     4261 ctgcttgtct taaaatctat gcttctcttt agtatctaaa attttcctag aagcttacaa
     4321 gtatataaat ggtctcttct caataaaggt tgtatattta ttcatcttat tgaatctgcc
     4381 atttcctcgt ttttgcgagt ttatatacct tccaattttc tttctattgt attttcaact
     4441 tctaatttta attcagggaa actgcttcaa cgcatcggta ccatggtctc caagggagag
     4501 gagctcttca ccggagtcgt cccaatcctc gtcgagctcg acggagacgt caacggacac
     4561 aagttctccg tccgtggaga gggagaggga gacgccacca acggaaagct caccctcaag
     4621 ttcatctgca ccaccggaaa gctcccagtc ccatggccaa ccctcgtcac caccctctcc
     4681 cacggagtcc agtgcttcgc ccgttaccca gaccacatga agcagcacga cttcttcaag
     4741 tccgccatgc cagagggata cgtccaggag cgtaccatct tcttcaagga cgacggaacc
     4801 tacaagaccc gtgccgaggt caagttcgag ggagacaccc tcgtcaaccg tatcgagctc
     4861 aagggagtcg acttcaagga ggacggaaac atcctcggac acaagctcga gtacaacttc
     4921 aactcccaca acatctacat catggccgtc aagcagaaga acggaatcaa ggtcaacttc
     4981 aagatccgtc acaacgtcga ggacggatcc gtccagctcg ccgaccacta ccagcagaac
     5041 accccaatcg gagacggacc agtcctcctc ccagactccc actacctctc cacccagtcc
     5101 gtcctctcca aggacccaaa cgagaagcgt gaccacatgg tcctcctcga gttccgtacc
     5161 gccgccggaa tcaccctcgg aatggacgag ctctacaagt gataagatat cacctcccac
     5221 aacgaggatt acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt
     5281 ggcatggatg aattgtataa gtaggagctc ccgagatctt cagcgctcag cattcgtaga
     5341 attgtaagtt taaactttct catactaact aacgcgctct atttaaattt tcagaattcc
     5401 aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc
     5461 tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca
     5521 gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc
     5581 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     5641 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     5701 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     5761 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     5821 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     5881 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     5941 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
     6001 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
     6061 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     6121 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     6181 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     6241 taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac
     6301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     6421 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6481 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
     6541 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8461 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8521 gcgcccaata c
//
