LOCUS       sequence-355715-        8340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression 
            of Chrimson::Cherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3577
FEATURES             Location/Qualifiers
     source          1..8340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2657..2668
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             3314..4021
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3456..3474)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3724..3743
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4940..4962)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5062..5081
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5159..5177)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5245..5349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5350..5418,5419..6210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5568..5587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6381..6969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6870..6889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7123..7140
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7257..7278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7293..7298,7299..7316,7317..7323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7331..7347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7336..7358
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7355..7371
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7355..7371
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgtgctccgt tacgtgaaaa acggaacaaa tattttttga aaaaaaaaca ttcgaatttt
       61 tccgtcgcgt ctcgaaatgc ccatttctcc tgctggtagg tttttataga cctctatttg
      121 aaaatttagt tatgaatgtt taaataagtg tctctaaaac tataaaaaac caatttttag
      181 aggaatcttg gtagatgctc acttttcagc tcacattgat taaatcctca aaagttcaca
      241 tataatcatt gaaattgttt tttgaaataa ttttttaaaa agttatgttt gactcattct
      301 gttttggtta ctttggcaac tacctgaaca cagtctcctg tatcaattgc tcttaaactt
      361 tttaatcgaa attttcagat tcaacaagca ttgctcgtca aaaacaacgt cgcatcgaac
      421 cacttgtaat gcttgattct cgggattacg gtagttggag gaatggaaca actccatcgc
      481 cgagcacaaa taacaaaagc cctccggtat ttcaaaatga gaagaagaga gttcattggc
      541 ggaaataatt cttgtttcaa tttttttttg tattctctgt aaactgtgta catattttgt
      601 aaattggatt actaatttaa aaataaatac ttgtaaaata aaatgaaata aataaataac
      661 tgtaaaagcg tacttcaggt agatccaaaa tatttggagt ggatttaatt tttgggttca
      721 ggtcaagtct gtgcggcaaa cggcacaagg ttcaagggac aagcaaccga aatcattgat
      781 actaacgaaa agttgaaatg cagtaagaag atcaaggaat taccggacaa aattattgcc
      841 aaggaacaaa ggcatacgtc tcagcttcat ttgcatgatc ctgttcaagt attccgtgac
      901 atcatcaacc accatcacgt gtgtacctca taccagggct gtgcggcatc ctgaattttc
      961 ggcgaacgtt cgccgacaca taaatttctc ataatttttc ggcgatcggc atttatcggc
     1021 acttttcggc atccttaaaa aaaaaatttt gtgttttttt ttcaattttc taaatctaaa
     1081 tagttgaatt ccaaagtttt ataaagagtg tctggtttga attttaaagc atagatcaat
     1141 ttcatattta aacgaccaga ctgttgggat agtcaaaaaa ggatcccaga ggcaagaatg
     1201 attcaaaata attgtgtcga ctaaaagaat atggctaata attcaaatat gttaattttt
     1261 gggaagtgtt agaaatacag gtgatttggg agtgttatga tacaagagac acagagatgt
     1321 ctttggttgt gttttatgac tataatgttt gtagatttat gtttcaacgg atgaatgtga
     1381 aaaacttgaa tgtatattcg attaaatttt taaaaaataa atttttcaca aatgccgaaa
     1441 aataccgaaa aaagtcgaac aaaagtcgac cgtttgccgc acatctctca tacatatgaa
     1501 cgtcatattt gaatattctc gtttaagctg atgtgcacgg catttggaat attacgggaa
     1561 ttgccaaaac catagaaaat attttcctaa cagcaatcga aactatttga ttccataact
     1621 ccaattccct tctttaaact agaaatttat aagcacatgc ctgttctaag tatccatcaa
     1681 tcaaatgcca agaaaccgca aaacaaggcg aaaattatca gaattctcaa caagtcatcc
     1741 gggcggctcc caagaggcag tgccgcggcg gcaacacgct catcttaaat gaaaacccaa
     1801 gaaagagacg ctatataatg ttttccgaaa tatcaatcat gcttctcctc acacatcagc
     1861 ttctcgtctt ctcgtcctac cgtagtcgct tatgaaccga gccgcctccc tagagactat
     1921 gggatcctca gcggtaagtg atgggctccg ccttgtaaaa tgcaacattt tccctcattc
     1981 aatatgagcc ggatgatttt tcgcatattt atcatttttg tttcaaagac tcctacagta
     2041 ctcttctaat cgtaaacctt ttcaattgac aataatttca gacccgatcc ctatggctga
     2101 gctcatctcc tccgctaccc gttccctctt cgctgctgga ggaatcaacc catggccgaa
     2161 cccataccac cacgaggaca tgggatgcgg aggaatgacc ccaaccggag agtgcttctc
     2221 caccgagtgg tggtgcgacc catcctacgg actctccgac gctggatacg gatactgctt
     2281 cgtcgaggct accggaggta agtttaaact tggacttact aactaaccat acatatttaa
     2341 attttcagga tacctcgtcg tcggagtcga gaagaagcag gcttggctcc actcccgtgg
     2401 aaccccagga gagaagatcg gagctcaggt ctgccagtgg atcgctttct ccatcgctat
     2461 cgctctcctc accttctacg gattctccgc ttggaaggct acctgcggat gggaggaggt
     2521 ctacgtctgc tgcgtcgagg tcctcttcgt caccctcgag atcttcaagg agttctcctc
     2581 cccagctacc gtctacctct ccaccggaaa ccacgcttac tgcctccgtt acttcgagtg
     2641 gctcctctcc tgcccagtca tcctcatccg tctctccaac ctctccggac tcaaggtaag
     2701 tttaaacagt tcggtactaa ctaaccctga ttatttaaat tttcagaacg actactccaa
     2761 gcgtaccatg ggactcatcg tctcctgcgt cggaatgatc gtcttcggaa tggctgctgg
     2821 actcgctacc gactggctca agtggctcct ctacatcgtc tcctgcatct acggaggata
     2881 catgtacttc caggctgcta agtgctacgt cgaggctaac cactccgtcc caaagggaca
     2941 ctgccgtatg gtcgtcaagc tcatggctta cgcttacttc gcttcctggg gttcctaccc
     3001 aatcctctgg gctgtcggac caggtaagtt taaacatata tatactaact aacggattat
     3061 atttaaattt tcagagggac tcctcaagct ctccccatac gctaactcca tcggacactc
     3121 catctgcgac atcatcgcta aggagttctg gaccttcctc gctcaccacc tccgtatcaa
     3181 gatccacgag cacatcctca tccacggaga catccgtaag accaccaaga tggagatcgg
     3241 aggagaggag gtcgaggtcg aggagttcgt cgaggaggag gacgaggaca ccgtcgcggt
     3301 acccgcggcc gccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3361 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3421 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3481 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3541 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     3601 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3661 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3721 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3781 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3841 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3901 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3961 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     4021 ataggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata
     4081 ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat
     4141 ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     4201 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     4261 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     4321 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     4381 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     4441 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     4501 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     4561 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     4621 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     4681 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     4741 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     4801 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     4861 tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt
     4921 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     4981 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     5041 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     5101 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc
     5161 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5221 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5281 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5341 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5401 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5461 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5521 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5581 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5641 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5701 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5761 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5821 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5881 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5941 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6001 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6061 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6121 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6181 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6241 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6301 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6361 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6421 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6481 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6541 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6601 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6661 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6721 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6781 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6841 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6901 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6961 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7021 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7081 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7141 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7201 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7261 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7321 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7381 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
     7441 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
     7501 aagcttgggc tgcagcatgc ggccgcttta gcgtatagta ttcggataga aatgtgtaaa
     7561 aatgatgaaa agcgtagcat tcaatgagac ccatatggaa attatgaaac cagaataatt
     7621 tggtaattgt attagtttag aaatatttgt ttaagttatt gtgttcaatt tgttttgttt
     7681 aggcagcaac tgatttggat gagaagagtt aaaaataggt tatttaatca ataaaagcaa
     7741 tatgaaaagt attcctcagt agggtctaac aataaataca ctaatggtca aaataatata
     7801 acttattcca gtctaaaaat attcacgaat tttcttctcc taaatactag aaattcttaa
     7861 atttatcaaa aaccagaaaa aattgcaaca tctacagtca gtttaaattg tttttgaatt
     7921 ttggtatttt ggtatttgag aaacgttcaa atcagatttt aaaaatattg gataggtatt
     7981 ttcgcactca tacatctttg aaataaaaca ttaagttttt tctcaatacg tttgaaaaaa
     8041 gtacagtact acatagtata tgcacacatt ttccaaatat atattatcaa cagaaaacta
     8101 ttctcatcat tctattttcc caatattccc atccactttg aatcactaac aacaatcatg
     8161 tgatttccta aatgttttcc ttttttcaaa ttgaagaaaa tcaacgctgc ggaacacatt
     8221 ttcccgaaaa aagccgtccg agttgttccc gtccacacct tttttccctc tcaagagtta
     8281 ttcttttgta gattctttgg atcattcact tgatagccta atagtgggcg tgtgtttgtc
//
