LOCUS       sequence-355716-        7335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans 
            A/B-MN-specific expression of miniSOG-SL2::RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3626
FEATURES             Location/Qualifiers
     source          1..7335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3001..3321
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(5538..5560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5660..5679
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5757..5775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5843..5947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5948..6016,6017..6808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6166..6185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6979..7335,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
      661 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      721 atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg ggctgcagca
      781 tgcggccgct caccaccgac tgattttcat ttcaaaatta atgcagattt ctacgtgttc
      841 tttttcaaat aaatttatat attttttgtt ctctcacaaa ataaataatt tcgagagtta
      901 agtgatcatt ctcagacata ttctataatt gtccgtcagg aaacagatag aggaaccttt
      961 gataaataaa ttataaaagg ccgacaggga aatgcttctc cctcgactct gttagaagcg
     1021 aagaggcaat ttttaaaaat ttcccatttt tatgcgtttt tctgatttcc aaagtcacgc
     1081 atcatttacc aaccgcaacg attcataagg acgtaatttt gattttctgg acgattttta
     1141 tatagttttg atgtgaaatt cgctttattt gtctgaaagt ggaatagatt tagtgtggat
     1201 ttaaccgaaa aatgcagatc ttttaaaaat ttaaaccagt gttttgttgc cttatttgcg
     1261 aaaaaaaatt gtgatatttc gagtattgct gcaatttgat tagtaaaatg ttaggatata
     1321 agtatttaaa aagtataatt ggttgaattt tctttttcat caccgacttc caaaaatata
     1381 gagatactga gttttcacca ttttttgaat gtaaataaaa acattttgaa cacttgtgct
     1441 gggattcaat ccaaatacat tcactgtcat ttaatattat tatatatcaa aataaaacgc
     1501 ataacttttt ctgttttttt ttaaattttt tcaaaatgta ggtttggtaa gttaaggtaa
     1561 ggttaggcaa tgtaggtaaa cacgagatga tactccacgt tgaaaattgt tttcataaat
     1621 acagtttaga ttgaactgaa ggattttatc gtctaataga atataatttt ttagttcaag
     1681 atttaggatt tgttttcact tttttttaga ttcaagacat atatctcgca aaatgagttt
     1741 tgtctttgag ccatcttgtc atcatagcac attgaatttt gttgttcgca agacggaata
     1801 tatgttccca gctgcaaatt ttgtgtcgga tatttcttct ttagcgaaag gcgttaaaaa
     1861 ccaaaaatag tcgaagcgtt tgatatgtgt cccgttggtt tttcgtgtcc atctattctg
     1921 tttcgagtac acatgttgcg acgcggcact gatgaaaaag tgaaagagct agtgtcacac
     1981 acaaacacaa agtatgctca tgtgagtgtg tagatatgtg tagatgtatg tcctgaagga
     2041 ataaaaagac tttttgaagc tacattttta aacaaaaaaa gacagaaaca tgaagcacaa
     2101 ctctctattc tgtcgaacgg cgtgtgcaac tcacgcacac ccaaaaatat gaaatataac
     2161 attcgcacca acaaagcgcg aatatcaatg aatctcgagg gaacgatggc tgcctgccgg
     2221 cgcgtgttgc tcttctgtca ttgggataac tggcgggtct gttgggtccg tcctttagac
     2281 catttgacta cactgccctg catatgtgtg aaacgtcgtc gttgctgttg ggagctgtct
     2341 gtgtgtatgt gtgtgtgtgt gagtatggag cagtaaaaag ctgccgatac gagaagcgcc
     2401 gccccggacg gacggcgatg gcgcatcgcc aaatgtgtca ttttagttgt cttcacgaac
     2461 cagagggccc acgacggacg ggcggccacg cccacctcaa ccagaatatc cttccttctc
     2521 ccacccaccc ttgcggacgc gcggatgcgc tgaacaaaaa gacgacgaag aagaagaaga
     2581 agaagctgaa cgaagaaaaa agcatcaaaa gaagtgaagc ggctctacca ccccgaccac
     2641 cttccttcac aggaaggacg ccgttttcat cccgggattg gccaaaggac ccaaaggtat
     2701 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga
     2761 cggatcccga tgtcggacac aattcttggt ttcaacaaat caaacgtcgt tttggctgct
     2821 ggaattgctg gagccgcttt cctcggctac tgcatttact tcgatcataa gagaatcaac
     2881 gctccagact acaaggacaa gattaggcaa agtcagtgtt ttaacaacat atttccttcg
     2941 gatttttatc taaaaacaac ttattttctt tcagagagac gtgcccaggc tggggatcct
     3001 atggagaaaa gtttcgtgat aactgatcca cggctgccag acaatcccat catcttcgca
     3061 tccgatggct tcctggagct gaccgagtat tccagagagg agatcctggg ccgcaatggc
     3121 cgctttctgc agggaccaga gacagaccag gccacagtgc agaagattcg cgatgccatt
     3181 agagatcagc gcgagattac cgtgcagctg ataaactaca caaaaagcgg gaagaaattc
     3241 tggaacctcc tgcacctcca gcccatgagg gaccagaagg gtgagctcca gtatttcatc
     3301 ggagtgcagc tggatggata aagatccccg agatcttcag cgctcagcat tcgtagaatt
     3361 gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca gaattcgctg
     3421 tctcatccta ctttcaccta gttaactgct tgtcttaaaa tctatgcttc tctttagtat
     3481 ctaaaatttt cctagaagct tacaagtata taaatggtct cttctcaata aaggttgtat
     3541 atttattcat cttattgaat ctgccatttc ctcgtttttg cgagtttata taccttccaa
     3601 ttttctttct attgtatttt caacttctaa ttttaattca gggaaactgc ttcaacgcat
     3661 cggtacccta tggtctcaaa gggtgaagaa gataacatgg caattattaa agagtttatg
     3721 cgtttcaagg tgcatatgga gggatctgtc aatgggcatg agtttgaaat tgaaggtgaa
     3781 ggagaaggcc gaccatatga gggaacacaa accgcaaaac taaaggtaag tttaaacata
     3841 tatatactaa ctaaccctga ttatttaaat tttcaggtaa ctaaaggcgg accattacca
     3901 ttcgcctggg acatcctctc tccacagttc atgtatggaa gtaaagctta tgttaaacat
     3961 ccggcagata taccagatta tttgaaactt tcattcccgg agggttttaa gtgggaacgc
     4021 gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc aagactcaag gtaagtttaa
     4081 acagttcggt actaactaac catacatatt taaattttca gcctccaaga tggagaattt
     4141 atttataaag tcaaacttcg aggaacgaat ttcccctcgg atggatctgt tatgcagaag
     4201 aagactatgg gatgggaagc ttcaagtgaa agaatgtacc ctgaagacgg tgctcttaag
     4261 ggagagatta aacaacgtct taaattgaaa gatggaggac attacgatgc tgaggtaagt
     4321 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggtgaa gacaacttac
     4381 aaagccaaaa aaccagttca gctgccagga gcgtacaatg ttaatattaa actggatatc
     4441 acctcccaca acgaggatta cactatcgtt gagcaatatg aaagagctga agggcggcac
     4501 tcgacaggtg gcatggatga attgtataag taggagctcc cgagatcttc agcgctcagc
     4561 attcgtagaa ttgtaagttt aaactttctc atactaacta acgcgctcta tttaaatttt
     4621 cagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat
     4681 aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt
     4741 aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc
     4801 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     4861 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     4921 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     4981 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     5041 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     5101 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     5161 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     5221 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc
     5281 aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     5341 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     5401 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     5461 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     5521 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5581 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5641 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     5701 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct
     5761 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5821 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5881 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5941 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6001 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6061 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6121 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6181 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6241 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6301 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6361 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6421 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6481 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6541 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6601 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6661 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6721 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6781 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6841 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6901 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6961 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7021 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7081 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7141 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7201 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7261 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7321 cagcttggag cgaac
//
