LOCUS       sequence-355749-        8698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D 
            premoter IN and others expression of TetanusToxin-UrSL-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2644
FEATURES             Location/Qualifiers
     source          1..8698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(36..41,42..59,60..66)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    74..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     79..101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     98..114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     98..114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4751..5461
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4896..4914)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5164..5183
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6377..6399)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6499..6518
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6600..6618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6686..6790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6791..6859,6860..7651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7009..7028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7822..8410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8311..8330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8564..8581
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(8698,1..21)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
       61 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      121 tacgccaagc ttgcatgcgc ggccgctgtg gtctcacttt gaacgacaaa agaaaaccaa
      181 atattgtaaa ggaatagtac agataaaagg gaacaagtga taccagctga tggatgtagg
      241 tcagcgacaa aagacaggag acgtatagtt tgatgattat ggaaccaaac tcagaattta
      301 atgttggaga cgctataaaa attgaattga gaaattgaaa ccatattctt gaatatctct
      361 caatttggaa tttggaagta gccgtaaaat cggctagcta gaacataaaa ggactgattt
      421 gcaaccttga ccattcatga tatcatttca ataaaagaag agattccaat gaccaatctt
      481 caagaatcac aataatcaca acaaaactaa gcaaaagtaa caacaaattg ttcaggtaga
      541 caagtagaca tttggacaca ttttgcgggg atgtgatgac tgtataataa aaataagtgg
      601 acactgagag agagaggagg tcagcaggaa aaaagaacat cgaactgctt gacgaattga
      661 aattagaatg aggtgtcagg caggtgacga ttacatggtt aattattagt gttctttttc
      721 ttctttttgt ggatgccgat tagttgaaat aattgcatgg aaagtgagaa ttcaatgtga
      781 atctctttct tattatgttt caagtggtat ctggatggaa gcttgtactt ttatgaatcg
      841 aaaattgcgc ccaaggttga ttttgagatt attcagtaaa tttttgtgct gattattcag
      901 ttaatgtaga acagagacgc ggatgaaaac ttttttaatt tctaaaaaaa aagacaaaat
      961 gtggttaaaa agtttggaaa ataattgtta tatacttctt gagagcttat tcaaacggaa
     1021 accaatcata aatagtcgaa tagtcattta gaaaagagaa actagttgat gtagagagat
     1081 tcaaaaagta aaatatgcaa atgatccgtt tcttagattt agtaaacatc taccaaatga
     1141 aacaggaatt gtcgaaacaa tttagaaaag gagaaggaaa catttgttta ttttcccctt
     1201 taaatggcgg tagaggtaaa acagcaatac acatagtaca aggttatact actttcaaaa
     1261 gaatttgaca tcccgccgag aaaatgtggt gattatatgg taaagttttc agttggatat
     1321 tatcttctac agacggtgat tcaaaatgat caaaagggca agtgggtggt aggatttgaa
     1381 aaattgagat atagaacgtg cgattttggt aaaacttttt gaacacaagg catatttttg
     1441 aactttgaaa aacgcaaaat ttaaattttg ttaattacat atattttgaa cagattatga
     1501 atataagcaa ggctacataa aatatgtata ttttcttttt acaaatattc gtacactgtt
     1561 aaatttatag aaaaatcatt tatattcaga aaaacctatt tgaacaattt ttagttgctc
     1621 aataaccaat ttgcacaaat ttagaaaata taatgggggc ttttaaaaat ttaaatcctc
     1681 agcgaatatt cctttgcgat tttttcgaac gttttctttc agattaaaaa atctgaagca
     1741 gaagtaaatg aactaccata gaaaattcac caaggatcac atttttacaa agcctccgcc
     1801 caattgtaaa cttgggaatt tttgcttgtt ttcttttttt tttcaaaaaa ggaatagttg
     1861 ttttaaagta atttattccc ctatgctttc tccagcaatc agttgttctc cccatacttt
     1921 acacaattat caacaacttc acatagaaaa caaaacaaca aatcagaaat tcccaccggt
     1981 cacagtcaca gattccccat gttttgttca tctcatttct gcaagaaagt gacatcgctg
     2041 ccacccaatt cgacaaattg tccaagtggt catcaggaag atatgacctt ctttttaatg
     2101 ataatttaga ctacaaaggg cgtcaaaaaa gattcaatga tcaggggtaa ttgctgtaaa
     2161 atgtagtttg tatgtgtgtt taccgtagcc tataactgta aaaattggtt ttttaaaaag
     2221 tatttctgag attaaaaggc tccccaagga tgcacgattc tagctagaaa tgaacattcc
     2281 atgggttgtg aagatgcttt tatctcactt tagaatgtct gactccgccc ctctgtgtac
     2341 gaattgaagt gctcttttgt gatgatcata gctcctcctt gcggcgacgc ccgcacacag
     2401 cacgagatat tacacggaat acttatgtga ttcataggaa acaaggaaat cgaaaaaata
     2461 ataggatttt gaggaggtat ttttagatga atgcttatga tgttatgatg aacccaagaa
     2521 attctgaata gacatatgtc agaacggggt aggagctcca gtgtcgcttt tgaaataaaa
     2581 aatcaagtgg tatacttctc gtatataccc ttctgaacta ataaaataat ttatcttttg
     2641 aaaaagtttg aaaaagaaaa atgcccaaaa cttgaatttt aatgttttgt attttgagca
     2701 tatcgttcaa acgtcgtgat ctgaaagtag aaagatctaa atattcgtga cacagtttgg
     2761 tttgaaggtc ttagtggtaa ttttttatta cagaagcaac aaaaagttga catattttca
     2821 gaatttccaa aaaaacgttt aaaatttcaa tttcttcgat agagttctaa aaattttttg
     2881 gaacggattt tgtaatatcc gctgataacc gttcaagaac aatttgaatt ttgaaataat
     2941 ttaatcaact agtatccagt gctctttctc actgctccac ccatttttca acccatttcc
     3001 tttactcaat ttcgcccgtt ttccttttgc tcaaatgtgc ccaccaaatc atctttcttc
     3061 gtcttctttt ctcttattcg gaaggagaag aggtgaaagt agaataagtt tttttgttga
     3121 aaagagagga gcacggccac ttttctctta tttccatcct tctactacct cgttttactt
     3181 tccatttaaa aacccgttta aagttccaaa atttcaatta tcttgcttct gtttggaaca
     3241 cgacaaactt tagttttgtt acagataccc gggattggcc aaaggaccca aaggtatgtt
     3301 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcatgccgat
     3361 caccatcaac aacttccgtt actccgaccc ggttaacaac gacaccatca tcatgatgga
     3421 accgccgtac tgcaaaggtc tagacatcta ctacaaagcc ttcaaaatca ccgaccgtat
     3481 ctggatcgtt ccggaacgtt acgaatttgg tactaaaccg gaagacttca acccgccgtc
     3541 ctccctgatc gaaggtgctt ccgaatacta cgacccgaac tacctgcgta ccgactccga
     3601 caaagaccgt ttcctgcaga ccatggttaa actgttcaac cgtatcaaaa acaacgttgc
     3661 tggtgaagct ctgctggaca aaatcatcaa cgctatcccg tacctgggta actcctactc
     3721 cctgctggac aaattcgaca ccaactccaa ctccgtttcc ttcaacctgc tggaacagga
     3781 tccgtccggt gctaccacca aatccgctat gctgaccaac ctgatcatct tcggtccggg
     3841 tccggttctg aacaaaaacg aagttcgtgg tatcgttctg cgtgttgaca acaaaaacta
     3901 cttcccgtgc cgtgacggtt tcggttccat catgcagatg gctttctgcc cggaatacgt
     3961 accgaccttc gacaacgtta tcgaaaacat cacctccctg accatcggta aatccaaata
     4021 cttccaggac ccggctctgc tgctgatgca cgaactgatc cacgttctgc acggtctgta
     4081 cggcatgcag gtttcctccc acgaaatcat cccgtccaaa caggaaatct acatgcagca
     4141 cacctacccg atctccgctg aagagctctt caccttcggt ggtcaggacg ctaacctgat
     4201 ctccatcgat atcaaaaacg acctgtacga aaaaaccctg aacgactaca aagctatcgc
     4261 taacaaactg tcccaggtta cctcctgcaa cgacccgaac atcgacatcg actcctacaa
     4321 acagatctat cagcagaagt atcagtttga taaggattcc aacggccagt atatagtgaa
     4381 cgaggataag tttcagatac tgtataactc cataatgtat ggctttactg agatagagct
     4441 gggcaagaag tttaacataa agactcgctt aagctatttt tccatgaacc acgatcctgt
     4501 gaagatacct aacctgctgg atgatactat atataacgat actgagggct ttaacataga
     4561 gtccaaggat ctgaagtccg agtataaggg ccagaacatg agggtgaaca ctaacgcctt
     4621 taggaacgtc gacggttccg gtctggtttc caaactgatc ggtctgtgca aaaaaatcat
     4681 cccgccgacc aacatccgtg aaaacctgta caaccgtacc gctagggtac cggtagaaaa
     4741 aatgctcgag atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     4801 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4861 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     4921 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4981 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     5041 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5101 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     5161 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     5221 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     5281 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     5341 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5401 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     5461 agaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     5521 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     5581 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     5641 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     5701 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     5761 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     5821 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     5881 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     5941 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     6001 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     6061 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     6121 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     6181 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     6241 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     6301 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     6361 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6421 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6481 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6541 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taattaaggg
     6601 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6781 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6841 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6901 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6961 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7021 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7081 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7141 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7201 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7261 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7321 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7381 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7441 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7501 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7561 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7621 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7681 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7741 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7801 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7861 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7921 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7981 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8041 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8101 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8161 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8221 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8281 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8341 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8401 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8461 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8521 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8581 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8641 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaatt
//
