LOCUS       sequence-355750-       11353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of 
            NLF::2XGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Griffin JK, Stagljar I, 
            Nagamatsu S, Zhen M
  TITLE     NLF-1 delivers a sodium leak channel to regulate neuronal 
            excitability and modulate rhythmic locomotion.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1069-82. doi: 
            10.1016/j.neuron.2013.01.018.
  PUBMED    23522043
REFERENCE   2  (bases 1 to 11353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.01"; journalName: "Neuron"; date: 
            "2013-03-20- 20"; volume: "77"; issue: "6"; pages: "1069-82"
COMMENT     Sequence Label: pJH2028
FEATURES             Location/Qualifiers
     source          1..11353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..74
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(89..94,95..112,113..119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    127..143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     132..154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     151..167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     151..167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     186..206
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        188..206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     243..259
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     243..259
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8685..8701)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8686..8702)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8726..8745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8727..8745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8755..8772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8755..8771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8764..8786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8913..9368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9000..9019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9210..9231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9394..9498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9499..9567,9568..10359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9717..9736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10530..11118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11019..11038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11272..11289
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
       61 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      181 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
      241 gctctagaac tagtggatcc ccctcgagag aagaggagga gctctttttc tctgcttccc
      301 catcactctc tccttctctc tctctcgttt acttttttcc agatcgatat ctatacattc
      361 cttttttgtc gttgtcctgg tttttcaatt tggttttgag catctcattt gttctgagtg
      421 gtgctctttt cgctgtcttt cgttttggtg tggtgtgtgt gtgtgtgtgt gctgcaatgt
      481 gtgtgcgttg ggatgccagc ggagagagga ggggggaaag aagagggatg tattggcgag
      541 ccgatggtgt gtattttcat gacatctttt cgcatgcctt ttgttttatt tatacgaaga
      601 tagacgtttt cacatttcga gagttgcttc ttgtgggaac actttttttt tcaaaaagtt
      661 atacattgtt tgcttcttga atttttttat atttttttta caaattgacc aactagttgt
      721 caatttttaa taaaaaacca taatctgaaa atagttttac tgattaccaa aatttcggtt
      781 aaaaatttga agatctgttc aaatgtttct ccaaactttt tgaattttta gtcttcggcc
      841 atactgagtt tttttttaat aaaaaaaata tatggacctg agactgtgaa actagtattc
      901 ataaaatata agaatttcaa aatgaatgtt atgttcccat attttttttt gtgtacagga
      961 aaaaaatgtt tctacatttt ttatacatag tgaaacaact ttaaaaaaat taaatatacc
     1021 ccagatacta gccatcatag tatagcacca caaatttatt ggtttatact agtagaaaac
     1081 ttcaaaaatt atgaaaacat ttaaataatt tggccgagca gcattgtttg atgtagattc
     1141 tagaatattc agtagtatat ataaataaca aaatctatgt gctctggcct atgaataacc
     1201 tataagcctt gaattttgaa ttgcagcgca atttgtgtct ggtaaccact tacgtttttt
     1261 tttaaacttt aaccccaaaa tacctctgag ttatttccgg tttgagcgct tgccggagcc
     1321 aaaagtcccc catgcatcaa agctcttgtg ttctgtttgt aaatcgacga aaatcttttt
     1381 tttcttcttg ctaacttcag atttttgcga aataatttaa aaatctacaa cttttttttc
     1441 agaaagtagt tagatgtgct ccgaagtgcg cgggctagcc tgacatgcgt ggattctccg
     1501 cgcacgatgc ggcctcgcac gccttacaca aaagaagcgc tacgatacga gcgtcgtcat
     1561 caaaagcctc cacccactca tccgcctgct cctacagctg ctcctcgtcc agacactaca
     1621 gaagtattcg gccggcgaga agcaaattcg tcgccgatca ccgattttat ccagtcgttt
     1681 tgtgcgagca ttcaagacga aagctgcagt tcaactgacg agccaaactt tgaattcaaa
     1741 aatgatatta ctgaacgatt atggttaaag ccttttgata ttgtgccttc gcctcaaatt
     1801 ttatccgatt cgaagaaaac tagttctcca agaaggtgct gggccaagaa ttttcgccga
     1861 tatttaaccg atgtgagttt gaaagtttta atggaaattt tgtgaaaacc attatatcat
     1921 taaatattga aacttttcag ggtattctta ttattcaatt atttacttta ttcaattgcg
     1981 cttcttcatc tcatttatcc aacacaattc ctccttgtaa aaattttggg ttttttgcgc
     2041 aggtatgtta atttggcacc ttaaaaggca agtccgttaa gaaaaataga tgcatattgt
     2101 ttcaaatttt ttaaaactct ttctatcaaa ataacagtaa agaagtgaga caaatttaaa
     2161 aaaatattta aatgcactga ccaaaactat caatgcactg accgaaacta tcaatgcact
     2221 gaccgaaact atcaatgcac tgaccgaaac tatcaatgca ctgacagaaa ctatcaatgc
     2281 actgacagaa actatcaatg cactgacaga aaagtcacag cattattcag cccaatatgt
     2341 gataaatcct tgattttctc ttgcaaattt tttttcagca tagcgacaat gtaactgatc
     2401 gttgcacagt ttggtctgat gacgtcccgc ggattaatat aattatgagt cgacaaggaa
     2461 ttcaagtacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa
     2521 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     2581 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     2641 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     2701 agccaacact tgtcactact ctcacttatg gtgttcaatg cttctcgaga tacccagatc
     2761 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     2821 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta
     2881 actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg
     2941 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     3001 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     3061 gaatcaaagt tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
     3121 agaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     3181 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     3241 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     3301 ttgtaacagc tgctgggatt acacatggca tggacgaact atacaaaagc ccacaagctt
     3361 cagggcccga gctcgacgtc ccgcggatta atataattat gagtcgacaa ggaattcaag
     3421 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     3481 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     3541 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     3601 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     3661 cacttgtcac tactctcact tatggtgttc aatgcttctc gagataccca gatcatatga
     3721 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     3781 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
     3841 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
     3901 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     3961 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     4021 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact
     4081 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     4141 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     4201 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     4261 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gcttcagggc
     4321 ccgagctcga cgtcattcct gaaatttgct cagctggcac acaatcacgt attagaatat
     4381 taaagcatgt gttccttttt ccagagttac aaccgttatc attttttgaa gtgagagatt
     4441 ttttcgttaa caattttcga tatttaatgg ttttgcagat attcgccgtc ggcttgagaa
     4501 acactgacac gtggcagttg aatgccggcg agaagggtaa tgattgcatt tcgggaagta
     4561 taaatcggtg tacatcgtgt ttccggagga tctcaggttt gttttcagca ggaaaatgca
     4621 aaaattaccc atcaactttt ttcaatccca ccttttaaaa ttgtgtgaat tagtgacaaa
     4681 cgaaaagaca attttctcga agtataaaag aggaagcagc aaatgaagac acgaattctt
     4741 gctcaaataa taatgaatat tgcttcaggc actcttcgta agcttaatga agcctatcgg
     4801 tctttcgaca caactctttc tcgtttcgac tgccttccag cggtggacac tgcctccgca
     4861 acaagaccgt tctcgccgaa cgcaacttgt gataattgta aagttagtgg ggggctctca
     4921 aataaaaagt gaatgtgata aaagggaacg attggacaaa atgattgaaa caatttggaa
     4981 acttgcaaaa attttaaaaa tgaaacatgt taaattgcat ttttgggcct gttaaaatag
     5041 tcgaatttaa atgttttaat ccaatgcact gacaaaatta ataagtgcac tgacaaaatt
     5101 aataagtgca ctgacaaaat taataagtgc actgacttca actgggtcgg taaagtacgt
     5161 atttttaagg caatttccaa attttaaagg atattagtct aatttcacat caaataaaaa
     5221 ttttaatttt tttttcaaag tatcactatt caaaatcttt taaaaactca tctttcgttt
     5281 tcgtaaagct ttttaaaaaa tttggcgttt tcttgatcga acttattttc tcagtttgtc
     5341 agtttattag gcccactttt atgaaatttt tatgaaaaaa aacttgaaaa tgatcaaaat
     5401 tcggtagtta catttgatct ttatttgtat tggaaagcta aagatctgag aaagcataca
     5461 aaaaaaattt gtttcagata tggtatcgta gatggttgtt ggttcaatcg cttcaaatct
     5521 ggcaacggcc tccgtgtatt aactggtgct actatactca attggcatgc ccacatctcg
     5581 cacctgcaaa attatgggac tacgcaggtc atccgtcgtt tcagtgtaga ggtaagaaaa
     5641 tttatcaggg agcatctcaa aagtacagga aatgttaaag tttcagtgag gggaaaacgg
     5701 aggaaatggt tttattgaaa tttatggttt ttaattgagt tcacaatttt ttcacaacca
     5761 atagttcgta taataaaaaa attcggttca agtagttttt agacgaatta cgccatgtag
     5821 gcaggcggcg tcacatttta aaattttttg gagttttact gaacagtggt agtatatatt
     5881 attaaaaaat cgaatcaaaa accaagtatc ttcaatttca tacttctatt ctatttaaac
     5941 cccaaagagc ttgtctactt cttttttctc cgagtttttc attctctatc tgtcacaatc
     6001 tacttatttt cgtgtgtcct ttgtgctgtt actcaatttc ctgaattctc aaaaacctcg
     6061 cctttgcatg tacttgactc gcacttctgg tccacttcca acgttttttt ttggacgacg
     6121 aatttgaatc caacaagaga ggaggagggg ttaggttttt gatggattaa aataggattt
     6181 aaagacgatc cgttcttcag gctgggggtg gtgatcccac cgggctttta agccacttcc
     6241 tagccgggga ctgtccttcc caatagaggc tgggtgaacc taggggtcag gccagacttg
     6301 aactcgtgac ctgcagcttt ctagcggtca ccactaccga ctgagctatc ccgcacctat
     6361 atgagaataa tattaggagt taaatggtat cgaattttta cttgttgagg gagtaagctc
     6421 aaagaggaaa aacctgaaag ttgaaatcaa ggtgcggaaa gttatgaata aatgtttttt
     6481 agctggaaaa aaatgtggtt aaactgaaaa tttgactaat ttaaatttga aatcatttca
     6541 cgcgggttat tgaaaaattt caattttttt caatcttacc ttcgacaaaa ccagaacatg
     6601 gaatacacga aaatatataa ctcttgctta taactactaa tatttaggtt gggtagagca
     6661 aaaaattcaa cggccggctt aaaaatttta aaatgaaact tgaaaacaga gacaaatata
     6721 tgaaaatttg tagatatgga cattgacagt tggcgccacg aatgtgactg catccatccg
     6781 tgtgatgttc gtggaattgt agctccaggt gttgcaatgg gcaacgctcc ttcagttcag
     6841 cacgactttt tcgcggcaca ggtttgttgg agtaaacgga actaaaattt gatacgaaat
     6901 cacaaattat atttggtaat tgccatatcc caaaaacttg aaactgtttg aaaaattttg
     6961 ccttcaccct tttcactatt ttttttactg agattcagga aaatattttt tcgatcaaat
     7021 cacttgtaag atttgaattt ttatctgttg aacttgatta atttaatgag aatgcacgaa
     7081 agagtataag taatttttta gataagtttc agtaaaatcg taacggtatt ttttttatat
     7141 ttttccaaaa attcgtaatt ttctgaaact aaccagacat tttttgatta attttttttt
     7201 catttttcta gaaaatagaa aaaaagttgt taaacactaa aagttctcag aaacgggcag
     7261 actttcaaaa ttctaccaaa attatctttc aattgttttt tatcaatgtc attgcttaaa
     7321 ctagattctg tttaacgttg aaattgtgca gatttcagcc cctagacttt ttttggatta
     7381 ttatgggttg acccaggtta gccaaaacat ttgaacttac catgacattt tttacaagta
     7441 taaacccaga taagtcaatt ttctgagact aaatgtaaac ctcacccacg ctttgattaa
     7501 ttttctattc ttaaaagaaa ttaaaaaaaa taaaccagca attcattttt gaaattcaac
     7561 tacttcacaa gtgtcaaata aaaacgccaa ttatgtctaa ttacagattc actgtgaaac
     7621 gcggaaaaag gagtgccaca agcgtttcaa acagattcgg aaatcaaaac ggagtgacac
     7681 cagcagtaac attgtttttt tatcaagcgc atcaccaaaa cgcccaaaat tcctgattct
     7741 ttcattgttg tttcttcgat ttatgttgtt gttatagtag attcttcatt catcgcctta
     7801 tacctttttt ccactcaatt atttttcaat ttctctaccc cttcttcaaa agtgtaattc
     7861 tcatgtgtca aagaaacaaa aaactcggct gatttgattc catttccttc attctttcca
     7921 tgttttcttt cttttttttt tcaaattttt tgaatcttgt ccaaaaccct tcaaactaaa
     7981 ctttctgtgc atttatatat ctagtacaat ttgaaacgtg atcgaaaatc aaattttgta
     8041 tgcccattcc ctcatccttc aaaatttcat ttactaacaa attttataaa ttccaaaaaa
     8101 agttcgcctg tcatctggac ttgtttgttt aggaaataat attagtatca acttgtgtca
     8161 attcgatttc tttctgagaa aatataaata cgcattgata attaaacctt gtttgtatcg
     8221 acacataaaa caatttggac agtgagatct cttggcgcca gccgagagcc gacttttttc
     8281 aagagccgca cgacactgca gtgcatagcc cccaccaggg ctgatttttt gttttttggt
     8341 tttttttgct ggttttttga gaccaaaaaa accaaaaaaa atcaaaaaat aaattacaac
     8401 actgattccg caaaaaatta aacaaagaag aacaaaaaac cttccgttcg tttacagaat
     8461 atttcatgac aattttccgt tttgtcactt ttgatatcaa acattgcatt tgtttgtttt
     8521 tagttttttc cttctttctc attcatttct tcattttttt ctcattttta tctttattga
     8581 ttcaacattt caaaaacttg tattttacat ctaaaactat aaaatttttc aaatcgagca
     8641 agatggggca ccatggggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc
     8701 gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8761 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8821 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8881 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     8941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9301 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9361 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10921 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    11161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    11221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    11281 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    11341 attcattaat gca
//
