LOCUS       sequence-355755-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Syt7 palmitoylation site mutant with 
            C terminal HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        141..470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     383..402
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        518..565
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             584..958
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1088..1209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1125..1144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1179..1198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1258..1274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1258..1274)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1271..1293)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1282..1298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1306..1312,1313..1330,1331..1336))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1351..1372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1489..1506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1660..2248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1740..1759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2419..3210,3211..3279))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3042..3061
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3280..3384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3459..3478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3650..4029
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4031..4229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4184..4204
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4247..4427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4474..4599
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5092..5325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5510..5554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5852..5969
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6014..6460
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             7749..7769
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    8715..9303
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8768..8788)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9186..9197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9306..9322)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9307..9323)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9828..10008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10034..10051)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10040..10264
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10310..10611,1..127)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10397..10416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(10607..10611,1..17)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
       61 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
      421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
      481 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
      541 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
      601 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
      661 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
      721 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
      781 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
      841 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
      901 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
      961 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     1021 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     1081 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1201 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     1261 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1321 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1381 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1441 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1501 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1561 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1621 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1681 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1741 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1801 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1861 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1921 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1981 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2041 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2101 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2161 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2221 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2281 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2341 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2401 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2461 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2521 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2581 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2641 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2701 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2761 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2821 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2881 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2941 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3001 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3061 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3121 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3181 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3241 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3301 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     3421 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     3481 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     3541 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     3601 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     3661 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     3721 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     3781 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     3841 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     3901 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     3961 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4021 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4081 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4141 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4201 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     4261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     4441 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     4501 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     4561 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     4621 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     4681 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     4741 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     4801 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     4861 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     4921 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     4981 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     5041 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     5101 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     5161 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     5221 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     5281 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     5341 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     5401 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     5461 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     5521 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     5581 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     5641 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     5701 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     5761 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     5821 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     5881 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     5941 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     6001 tttggttaat taaagtgcaa gtggttttag gaccaggatg aggcggggtg ggggtgccta
     6061 cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca
     6121 tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc
     6181 ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag
     6241 cactgaaggc gcgctgacgt cactcgccgg tcccccgtaa actccccttc ccggccacct
     6301 tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg
     6361 ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc
     6421 ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag gtacggctag cggatccggt
     6481 accgcgggcc cgccaccatg taccgggacc cggaggcggc cagcccaggg gcacctaccc
     6541 gcgatgtcct gctggtctct gcaatcatca ccgtcagcct tagcgtcact atcgtcctcg
     6601 caggcctggc ccactgggct cagcgcaaac tgggcaaacg ctacaagaat tccttggaga
     6661 cggtgggcac gccagactcg gggcgtgggc gcggtgagaa gaaagccatc aagttgcctg
     6721 caggagggaa ggctgtgaat acagccccag tgcccggcca gacgccacac gatgagtctg
     6781 accgcagaac ggagacccgt tcctctgtct cggacctcgt caactccctt accagcgaga
     6841 tgctcatgct ctccccaggt tctgaggagg atgaggccca cgagggctgc agccgagaga
     6901 acctgggccg aatccagttc agtgttggct acaacttcca agagtccaca ctcaccgtga
     6961 aggtcatgaa ggcccaagag ctgccagcca aggacttcag cggtactagt gacccctttg
     7021 tcaagatcta cctgctaccc gacaagaagc acaaactgga gaccaaggtg aagcggaaga
     7081 atctaaaccc gcactggaat gagacctttc tatttgaagg gtttccctac gagaaagtgg
     7141 tgcagagggt cctctacctc caggtcctgg attatgaccg tttcagccgc aatgacccca
     7201 ttggggaggt gtccatccct ctgaacaagg tggacctgac ccagatgcag accttctgga
     7261 aggatctgaa gccatgcagc gatgggagtg ggagccgagg ggagctgctc ttgtccctct
     7321 gctacaaccc ctctgccaac tccatcatcg tgaacatcat caaagctcga aacctcaaag
     7381 ccatggacat cgggggcaca tcagacccct atgtgaaggt gtggctgatg tataaagaca
     7441 agcgggtaga gaaaaagaag actgtgacaa agaagaggaa cctgaacccc atcttcaatg
     7501 agtctttcgc cttcgacata cccacggaga agctgaggga gaccacgatc atcatcactg
     7561 tcatggacaa agacaagctc agccgcaacg acgtcatcgg caagatctac ctgtcctgga
     7621 agagcggacc aggtgaagtg aaacactgga aggacatgat cgctcgtccc cggcagcctg
     7681 tggcccagtg gcaccagctg aaagccggat ccggaagttc ggggtccagc ggttcttcag
     7741 gcagttccga ggatctgtac tttcagagcg ataacgatgg atccgaaatc ggtattggct
     7801 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     7861 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     7921 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     7981 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     8041 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     8101 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     8161 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     8221 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     8281 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     8341 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     8401 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     8461 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     8521 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     8581 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     8641 gctggctgtc tactctggag atttccggtt aaggccgcac tcctcagcta gaattcgata
     8701 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8761 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8821 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8881 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8941 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9001 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9061 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9121 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9181 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9241 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9301 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     9361 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     9421 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     9481 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9541 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     9601 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     9661 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     9721 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     9781 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     9841 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9901 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9961 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
    10021 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    10081 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    10141 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    10201 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
    10261 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
    10321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    10381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg a
//
