LOCUS       sequence-355757-       10398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn LAMP1-msGFP (JV012)
FEATURES             Location/Qualifiers
     source          1..10398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(74..93)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        265..644
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        646..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     799..819
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             862..1042
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1089..1214
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1707..1940
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2125..2169
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2467..2584
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2629..3075
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3107..3116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3111..3120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3113..3175,3176..4333)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     primer_bind     complement(4418..4439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5026..5047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5117..5705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5170..5190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5588..5599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5708..5724)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5709..5725)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6230..6410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6436..6453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6442..6666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6712..7140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6799..6818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7009..7030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7149..7169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7154..7483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7334..7469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7396..7415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7531..7578
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7597..7971
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8101..8222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8138..8157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8192..8211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8271..8287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8271..8287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8284..8306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8295..8311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8319..8325,8326..8343,8344..8349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8364..8385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8502..8519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8673..9261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8753..8772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9432..10223,10224..10292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10055..10074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10293..10397)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
       61 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
      121 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
      181 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
      241 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
      301 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      361 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      421 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      481 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      541 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      601 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      661 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      721 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      781 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      841 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      901 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      961 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1021 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     1081 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     1141 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     1201 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1261 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1321 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1381 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1441 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1501 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1561 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1621 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1681 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1741 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1801 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1861 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1921 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1981 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     2041 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     2101 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     2161 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     2221 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2281 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2341 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2401 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2461 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2521 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2581 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaaag tgcaagtggt
     2641 tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg
     2701 gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga
     2761 aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg
     2821 cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc
     2881 gccggtcccc cgtaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc
     2941 ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc
     3001 tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg
     3061 tgcctgagag cgcaggtacg gctagcggat ccggtaccgc gggcccgcca ccatggcggc
     3121 cccgggcgcc cggcggccgc tgctcctgtt gctgctggca ggccttgcac acagcgcccc
     3181 agcactgttc gaggtgaaag acaacaacgg cacagcgtgt ataatggcca gcttctctgc
     3241 ctcctttctg accacctatg aggctggaca tgtttctaag gtctcgaata tgaccctgcc
     3301 agcctctgca gaagtcctga agaatagcag ctcttgtggt gaaaagaatg cttctgagcc
     3361 caccctcgca atcacctttg gagaaggata tttactgaaa ctcaccttca caaaaaacac
     3421 aacacgttac agtgtccagc acatgtattt cacatataac ctgtcagaca cacaattctt
     3481 tcccaatgcc agctccaaag ggcccgacac tgtggattcc acaactgaca tcaaggcaga
     3541 catcaacaaa acataccgat gtgtcagcga catcagggtc tacatgaaga atgtgaccat
     3601 tgtgctctgg gacgctacta tccaggccta cctgccgagt agcaacttca gcaaggaaga
     3661 gacacgctgc ccacaggatc aaccttcccc aactactggg ccacccagcc cctcaccacc
     3721 acttgtgccc acaaacccca gtgtgtccaa gtacaatgtg actggtgaca atggaacctg
     3781 cctgctggcc tctatggcac tgcaactcaa catcacctac atgaagaagg acaacacgac
     3841 tgtgaccaga gcattcaaca tcaacccaag tgacaaatat agtgggactt gcggtgccca
     3901 gttggtgacc ctgaaggtgg ggaacaagag cagagtcctg gagctgcagt ttgggatgaa
     3961 tgccacttct agcctgtttt tcctgcaagg agttcagttg aacatgactc ttcctgatgc
     4021 catagagccc acgttcagca cctccaacta ttccctgaaa gctcttcagg ccagtgtcgg
     4081 caactcatac aagtgcaaca gtgaggagca catctttgtc agcaaggcgc tcgccctcaa
     4141 tgtcttcagc gtgcaagtcc aggctttcag ggtagaaagt gacaggtttg ggtctgtgga
     4201 agagtgtgta caggacggta acaacatgct gatccccatt gctgtgggcg gggccctggc
     4261 agggctggtc ctcatcgtcc tcatcgccta cctcatcggc aggaagagga gtcacgcggg
     4321 ctatcagacc atcggatccg gaagttcggg gtccagcggt tcttcaggca gttccatgga
     4381 tagcactgag agcctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4441 aaacggccac aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct
     4501 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4561 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     4621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatca ccttcaagga
     4681 cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4801 gtacaacttc aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     4861 ggccaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta
     4921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4981 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5041 gttcgtgacc gccgccggga tcactctcgg ctaaggccgc actcctcagc tagaattcga
     5101 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5161 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5221 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5281 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5341 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5401 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5461 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5521 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5581 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5641 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5701 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     5761 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5821 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5881 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5941 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     6001 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     6061 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6121 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     6181 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     6241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     6421 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6481 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6541 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6601 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6661 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7141 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7501 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7561 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7621 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7681 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7741 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7801 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7861 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7921 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     7981 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8041 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8101 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8161 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8221 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8401 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8461 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8521 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10321 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10381 caaatagggg ttccgcgc
//
