LOCUS       sequence-35577-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNN1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..26)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     775..794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
       61 tacattgcac aagataaaaa tatatcatca tgcctcctct agaggtctcg ctatcgccag
      121 caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
      181 ggaccatggg agaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
      241 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      421 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      661 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      841 gtgatgctcg tcaggggggc ggagcctatg
//
