LOCUS       sequence-35578-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pLR-NI
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     95..112
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     96..112
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
       61 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      121 cgggcccgtc tcgctatcgc cagcaacatt ggcggcaagc aagcgctcga aagcattgtg
      181 gcccagctga gccggcctga tccggcgttg gccgcgttga ccaacgagag acgtctagac
      241 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
      301 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
      361 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      421 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
      481 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
      541 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
      601 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      661 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      721 gccacacagt gatattgatt tgctggttac ggtgaccgta a
//
