LOCUS       sequence-355784-       10083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYP-mRuby3
FEATURES             Location/Qualifiers
     source          1..10083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    207..324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        369..815
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      847..856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2542..3130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2595..2615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3013..3024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3133..3149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3134..3150)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3655..3835
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3861..3878)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3867..4091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4137..4565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4224..4243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4434..4455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4574..4594)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4579..4908
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4759..4894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4821..4840
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4956..5003
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5022..5396
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5526..5647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5563..5582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5617..5636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5696..5712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5696..5712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5709..5731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5720..5736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5744..5750,5751..5768,5769..5774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5789..5810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5927..5944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6098..6686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6178..6197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6857..7648,7649..7717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7480..7499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7718..7822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7897..7916)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8088..8467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8469..8667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8622..8642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8685..8865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8912..9037
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9530..9763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9948..9992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
       61 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
      121 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
      181 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      241 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      301 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      361 ttaattaaag tgcaagtggt tttaggacca ggatgaggcg gggtgggggt gcctacctga
      421 cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
      481 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
      541 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
      601 aaggcgcgct gacgtcactc gccggtcccc cgtaaactcc ccttcccggc caccttggtc
      661 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
      721 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
      781 gcagcggagg agtcgtgtcg tgcctgagag cgcaggtacg gctagcggat ccggtaccgc
      841 gggcccgcca ccatggacgt ggtgaatcag ctggtggctg ggggtcagtt ccgggtggtc
      901 aaggagcccc ttggcttcgt gaaggtgctg cagtgggtct ttgccatctt cgcctttgct
      961 acgtgtggca gctacaccgg ggagcttcgg ctgagcgtgg agtgtgccaa caagacggag
     1021 agtgccctca acatcgaagt tgaattcgag taccccttca ggctgcacca agtgtacttt
     1081 gatgcaccct cctgcgtcaa agggggcact accaagatct tcctggttgg ggactactcc
     1141 tcgtcggctg aattctttgt caccgtggct gtgtttgcct tcctctactc catgggggcc
     1201 ctggccacct acatcttcct gcagaacaag taccgagaga acaacaaagg gcctatgatg
     1261 gactttctgg ctacagccgt gttcgctttc atgtggctag ttagttcatc agcctgggcc
     1321 aaaggcctgt ccgatgtgaa gatggccacg gacccagaga acattatcaa ggagatgccc
     1381 atgtgccgcc agacagggaa cacatgcaag gaactgaggg accctgtgac ttcaggactc
     1441 aacacctcag tggtgtttgg cttcctgaac ctggtgctct gggttggcaa cttatggttc
     1501 gtgttcaagg agacaggctg ggcagcccca ttcatgcgcg cacctccagg cgccccggaa
     1561 aagcaaccag cacctggcga tgcctacggc gatgcgggct acgggcaggg ccccggaggc
     1621 tatgggcccc aggactccta cgggcctcag ggtggttatc aacccgatta cgggcagcca
     1681 gccagcggtg gcggtggcta cgggcctcag ggcgactatg ggcagcaagg ctatggccaa
     1741 cagggtgcgc ccacctcctt ctccaatcag atgaaaggat ccggaagttc ggggtccagc
     1801 ggttcttcag gcagttccat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt
     1861 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
     1921 gaaggcagac cgtacgaggg agtgcaaacc atgaggatca aagtcatcga gggaggaccc
     1981 ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg tacctttatc
     2041 aagtacccgg ccgacatccc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg
     2101 gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcacgcagga caccagcctt
     2161 gaggatggcg agctcgtcta caacgtcaag gtcagagggg taaactttcc ctccaatggt
     2221 cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca
     2281 gatggtggtc tgagaggata cactgacatc gcactgaaag ttgatggtgg tggccatctg
     2341 cactgcaact tcgtgacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc
     2401 ggtgtccatg ccgttgatca ccgcctggaa aggatcgagg agagtgacaa tgaaacctac
     2461 gtagtgcaaa gagaagtggc agttgccaaa tacagcaacc ttggtggtgg ctaactagaa
     2521 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     2581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2641 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2701 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2761 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2821 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2881 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2941 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3001 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3061 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3121 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     3181 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     3241 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     3301 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3361 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     3421 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     3481 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     3541 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     3601 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     3661 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3721 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3781 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     3841 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3901 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3961 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4021 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4081 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4141 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4201 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4261 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4321 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4381 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4441 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4501 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4561 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4621 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4681 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4741 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4801 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4861 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4921 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4981 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     5041 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     5101 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     5161 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     5221 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     5281 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     5341 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     5401 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5461 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5521 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5641 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5701 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5761 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5821 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5881 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5941 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6481 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6721 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7141 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7441 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7741 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7801 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7861 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7921 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7981 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     8041 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     8101 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8161 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8221 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8281 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8341 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8401 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8461 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8521 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8581 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8641 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     8701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8881 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8941 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9001 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9061 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9121 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9181 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9241 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9301 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9361 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9421 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9481 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9541 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9601 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9661 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9721 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9781 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9841 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9901 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9961 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10021 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10081 ttg
//
