LOCUS       sequence-355785-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of iGluSnFR biosensor for monitoring synaptic 
            vesicle exocytosis and glutamate release.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) CamKII sf iGluSnFR S72A
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      183..318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     245..264
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        380..427
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             446..820
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    950..1071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(987..1006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1041..1060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1120..1136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1120..1136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1144..1160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1168..1174,1175..1192,1193..1198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1213..1234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1351..1368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1522..2110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1602..1621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2281..3072,3073..3141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2904..2923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3142..3246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3321..3340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3512..3891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3893..4091
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4046..4066
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4109..4289
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4336..4461
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4954..5187
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5372..5416
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5714..5831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5886..7174
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      7190..7199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     7196..7255
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     8224..8245
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8348..8369)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             8834..8863
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    9098..9686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9151..9171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9569..9580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9689..9705)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9690..9706)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10211..10391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10417..10434)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10423..10647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10693..11121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10780..10799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10990..11011
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(11130..11132,1..18))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
       61 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      241 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      301 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      361 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      421 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      481 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      541 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      601 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      661 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      721 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      781 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      841 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      901 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
      961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1081 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3241 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3301 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3361 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3421 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3481 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3541 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3901 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3961 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4021 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4081 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4261 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4321 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4381 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4441 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4501 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4561 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4621 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4681 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4741 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4801 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4861 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4921 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     4981 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5041 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5101 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5161 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5221 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5281 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5341 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5401 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5461 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5521 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5581 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5641 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5701 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5761 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5821 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gaggggtcgg
     5881 caattcatta tggccttagg tcacttcatc tccatggggt tcttcttctg attttctaga
     5941 aaatgagatg tgggtgcaga gagcttcctc agtgacctgc ccagggtcac atcagaaatg
     6001 tcagagctag aacttgaact cagattacta atcttaaatt ccatgccttg ggggcatgca
     6061 agtacgatat acagaaggag tgaactcatt agggcagatg accaatgagt ttaggaaaga
     6121 agagtccagg gcagggtaca tctacaccac ccgcccagcc ctgggtgagt ccagccacgt
     6181 tcacctcatt atagttgcct ctctccagtc ctaccttgac gggaagcaca agcagaaact
     6241 gggacaggag ccccaggaga ccaaatcttc atggtccctc tgggaggatg ggtggggaga
     6301 gctgtggcag aggcctcagg aggggccctg ctgctcagtg gtgacagata ggggtgagaa
     6361 agcagacaga gtcattccgt cagcattctg ggtctgtttg gtacttcttc tcacgctaag
     6421 gtggcggtgt gatatgcaca atggctaaaa agcagggaga gctggaaaga aacaaggaca
     6481 gagacagagg ccaagtcaac cagaccaatt cccagaggaa gcaaagaaac cattacagag
     6541 actacaaggg ggaagggaag gagagatgaa ttagcttccc ctgtaaacct tagaacccag
     6601 ctgttgccag ggcaacgggg caatacctgt ctcttcagag gagatgaagt tgccagggta
     6661 actacatcct gtctttctca aggaccatcc cagaatgtgg cacccactag ccgttaccat
     6721 agcaactgcc tctttgcccc acttaatccc atcccgtctg ttaaaagggc cctatagttg
     6781 gaggtggggg aggtaggaag agcgatgatc acttgtggac taagtttgtt cgcatcccct
     6841 tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc ctgggcccac
     6901 acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt ttaaagtgaa
     6961 aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg tttcggaggt
     7021 ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca gccacagtgc
     7081 cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac tcaggagcac
     7141 gggcaggcga gtggccccta gttctggggg cagccggtac cgcgggcccg ccaccatgga
     7201 gacagacaca ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacag
     7261 atctgccgca ggcagcacgc tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca
     7321 ccgtgaatct tcagtgcctt tctcttatta cgacaatcag caaaaagtgg tgggttactc
     7381 gcaggattac tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca aaccggactt
     7441 gcaggtaaaa ctgattccga ttaccgcaca aaaccgtatt ccactgctgc aaaacggcac
     7501 tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt
     7561 ctctgacact attttcgtgg tcggtacgcg cctgttgacc aaaaagggtg gcgatatcaa
     7621 agattttgcc aacctgaaag acaaagccgt agtcgtcact tccggcacta cctctgaagt
     7681 tttgctcaac aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga
     7741 tcacggtgac tctttccgca ccctggaaag cggtcgtgcc gttgccttta tgatggatga
     7801 cgtgctgctg gccggtgaac gtgcgaaagc gaagaaacca gacaactggg aaatcgtcgg
     7861 caagccgcag tctcaggagg cctacggttg tatgttgcgt aaagatgatc cgcagttcaa
     7921 aaagctgatg gatgacacca tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt
     7981 tgataagtgg ttcaaaaatc caattctggt gagccacaac gtctatatca ccgccgacaa
     8041 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacgtggagg acggcagcgt
     8101 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     8161 cgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg agaagcgcga
     8221 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     8281 gtacaagggc ggtaccggag ggagcatgag caagggcgag gagctgttca ccggggtggt
     8341 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga
     8401 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     8461 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     8521 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     8581 cgtccaggag cgcaccatca gcttcaagga cgacggcacc tacaagaccc gcgccgaggt
     8641 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     8701 ggacggcaac atcctggggc acaagctgga gtacaacttt aacaaccccc tgaacatgaa
     8761 tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa
     8821 actgcaggtc gacgaacaaa aactcatctc agaagaggat ctgaatgctg tgggccagga
     8881 cacgcaggag gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg tgatctcagc
     8941 catcctggcc ctggtggtgc tcaccatcat ctcccttatc atcctcatca agtacctgca
     9001 gaaaagaagg gagcggagac gccaggagaa cgcaaacagc ttttgctatg aaaatgaagt
     9061 tgccctctaa ctagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     9121 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     9181 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     9241 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     9301 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     9361 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     9421 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     9481 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     9541 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     9601 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     9661 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     9721 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     9781 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     9841 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9901 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     9961 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
    10021 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
    10081 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
    10141 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
    10201 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10261 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10321 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10381 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
    10441 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    10501 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    10561 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    10621 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    10681 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    10741 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    10801 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10861 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    10921 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10981 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    11041 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    11101 aacaaaaatt taacgcgaat taattctgtg ga
//
