LOCUS       sequence-355787-        8750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) U6-SYT7 sgRNA 777 HaloTag
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        294..673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        675..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     828..848
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             891..1071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1118..1243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1736..1969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2154..2198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2496..2613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2682..2930
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2682..2702
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2853..2872
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2952..3027
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3169..4053
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEIS"
     LTR             4611..4791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4817..4834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4823..5047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5093..5521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5180..5199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5390..5411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5530..5550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5535..5864
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5715..5850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5777..5796
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5912..5959
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5978..6352
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6482..6603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6519..6538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6573..6592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6652..6668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6652..6668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6665..6687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6676..6692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6700..6706,6707..6724,6725..6730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6745..6766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6883..6900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7054..7642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7134..7153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7813..8604,8605..8673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8436..8455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8674..8750,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
       61 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
      121 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      181 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      241 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      301 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      661 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      721 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      781 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      841 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1081 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1141 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1201 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1261 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1321 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1381 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1441 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1501 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1561 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1621 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1681 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1741 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1801 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1861 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1921 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1981 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2041 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2101 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2161 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2221 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2281 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2341 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2401 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     2461 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2521 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2581 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2641 ccagtttggt taggcctttt gctggccttt tgctcacatg tgagggccta tttcccatga
     2701 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
     2761 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     2821 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     2881 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gcaccagctg
     2941 aaagcctgag agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3001 cttgaaaaag tggcaccgag tcggtgcttt tttgttttag agctagaaat agcaagttaa
     3061 aataaggcta gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg ctctagaagc
     3121 tcttaccctc tcaggctttc agctggtgga tcggggtcca gcggaagtat ggaaatcggt
     3181 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
     3241 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
     3301 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
     3361 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
     3421 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
     3481 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
     3541 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc
     3601 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
     3661 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
     3721 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc
     3781 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
     3841 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
     3901 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
     3961 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
     4021 atcgcgcgct ggctgtctac tctggagatt tcctgacgcg accctctcag gctttcagct
     4081 ggtgctctcg aggatcctag gatcgagacc tagaaaaaca tggagcaatc acaagtagca
     4141 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     4201 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4261 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4321 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     4381 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     4441 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     4501 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     4561 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     4621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     4801 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4861 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4921 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4981 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5041 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5101 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5161 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5221 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5281 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5341 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5401 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5461 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5521 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5581 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5641 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5701 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5761 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5821 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5881 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     5941 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     6001 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     6061 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     6121 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     6181 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     6241 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     6301 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     6361 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6421 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6481 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6541 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6601 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6661 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6721 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6781 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6841 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7921 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
//
