LOCUS       sequence-355788-       11706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..90)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        75..404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      255..390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     317..336
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        452..499
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             518..892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1022..1143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1059..1078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1113..1132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1205..1227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1216..1232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1240..1246,1247..1264,1265..1270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1285..1306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1423..1440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1594..2182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1674..1693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2353..3144,3145..3213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2976..2995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3214..3318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3393..3412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3584..3963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3965..4163
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4118..4138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4181..4361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4408..4533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5026..5259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5444..5488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5786..5903
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5948..6394
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      6426..6435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6430..6439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6432..6494,6495..7652)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             8778..8798
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             8814..9698
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    9744..10332
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9797..9817)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10215..10226)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10335..10351)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10336..10352)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10857..11037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11063..11080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11069..11293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11339..11706,1..61)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11426..11445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11636..11657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
       61 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      421 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
      481 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
      541 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      601 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      661 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      721 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      781 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      841 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
      901 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
      961 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1141 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1201 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1261 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1381 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2701 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2761 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2821 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2881 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2941 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3001 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3061 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3121 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3181 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3241 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3301 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3361 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3421 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3481 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3541 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3601 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3661 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3721 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3781 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3841 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3901 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4141 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     4201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     4801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     4861 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     4921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     4981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5641 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     5701 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     5761 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     5821 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     5881 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     5941 taattaaagt gcaagtggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
     6001 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
     6061 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
     6121 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
     6181 aggcgcgctg acgtcactcg ccggtccccc gtaaactccc cttcccggcc accttggtcg
     6241 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
     6301 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
     6361 cagcggagga gtcgtgtcgt gcctgagagc gcaggtacgg ctagcggatc cggtaccgcg
     6421 ggcccgccac catggcggcc ccgggcgccc ggcggccgct gctcctgttg ctgctggcag
     6481 gccttgcaca cagcgcccca gcactgttcg aggtgaaaga caacaacggc acagcgtgta
     6541 taatggccag cttctctgcc tcctttctga ccacctatga ggctggacat gtttctaagg
     6601 tctcgaatat gaccctgcca gcctctgcag aagtcctgaa gaatagcagc tcttgtggtg
     6661 aaaagaatgc ttctgagccc accctcgcaa tcacctttgg agaaggatat ttactgaaac
     6721 tcaccttcac aaaaaacaca acacgttaca gtgtccagca catgtatttc acatataacc
     6781 tgtcagacac acaattcttt cccaatgcca gctccaaagg gcccgacact gtggattcca
     6841 caactgacat caaggcagac atcaacaaaa cataccgatg tgtcagcgac atcagggtct
     6901 acatgaagaa tgtgaccatt gtgctctggg acgctactat ccaggcctac ctgccgagta
     6961 gcaacttcag caaggaagag acacgctgcc cacaggatca accttcccca actactgggc
     7021 cacccagccc ctcaccacca cttgtgccca caaaccccag tgtgtccaag tacaatgtga
     7081 ctggtgacaa tggaacctgc ctgctggcct ctatggcact gcaactcaac atcacctaca
     7141 tgaagaagga caacacgact gtgaccagag cattcaacat caacccaagt gacaaatata
     7201 gtgggacttg cggtgcccag ttggtgaccc tgaaggtggg gaacaagagc agagtcctgg
     7261 agctgcagtt tgggatgaat gccacttcta gcctgttttt cctgcaagga gttcagttga
     7321 acatgactct tcctgatgcc atagagccca cgttcagcac ctccaactat tccctgaaag
     7381 ctcttcaggc cagtgtcggc aactcataca agtgcaacag tgaggagcac atctttgtca
     7441 gcaaggcgct cgccctcaat gtcttcagcg tgcaagtcca ggctttcagg gtagaaagtg
     7501 acaggtttgg gtctgtggaa gagtgtgtac aggacggtaa caacatgctg atccccattg
     7561 ctgtgggcgg ggccctggca gggctggtcc tcatcgtcct catcgcctac ctcatcggca
     7621 ggaagaggag tcacgcgggc tatcagacca tccgcaaact gggcaaacgc tacaagaatt
     7681 ccttggagac ggtgggcacg ccagactcgg ggcgtgggcg cggtgagaag aaagccatca
     7741 agttgcctgc aggagggaag gctgtgaata cagccccagt gcccggccag acgccacacg
     7801 atgagtctga ccgcagaacg gagacccgtt cctctgtctc ggacctcgtc aactccctta
     7861 ccagcgagat gctcatgctc tccccaggtt ctgaggagga tgaggcccac gagggctgca
     7921 gccgagagaa cctgggccga atccagttca gtgttggcta caacttccaa gagtccacac
     7981 tcaccgtgaa ggtcatgaag gcccaagagc tgccagccaa ggacttcagc ggtactagtg
     8041 acccctttgt caagatctac ctgctacccg acaagaagca caaactggag accaaggtga
     8101 agcggaagaa tctaaacccg cactggaatg agacctttct atttgaaggg tttccctacg
     8161 agaaagtggt gcagagggtc ctctacctcc aggtcctgga ttatgaccgt ttcagccgca
     8221 atgaccccat tggggaggtg tccatccctc tgaacaaggt ggacctgacc cagatgcaga
     8281 ccttctggaa ggatctgaag ccatgcagcg atgggagtgg gagccgaggg gagctgctct
     8341 tgtccctctg ctacaacccc tctgccaact ccatcatcgt gaacatcatc aaagctcgaa
     8401 acctcaaagc catggacatc gggggcacat cagaccccta tgtgaaggtg tggctgatgt
     8461 ataaagacaa gcgggtagag aaaaagaaga ctgtgacaaa gaagaggaac ctgaacccca
     8521 tcttcaatga gtctttcgcc ttcgacatac ccacggagaa gctgagggag accacgatca
     8581 tcatcactgt catggacaaa gacaagctca gccgcaacga cgtcatcggc aagatctacc
     8641 tgtcctggaa gagcggacca ggtgaagtga aacactggaa ggacatgatc gctcgtcccc
     8701 ggcagcctgt ggcccagtgg caccagctga aagccggatc cggaagttcg gggtccagcg
     8761 gttcttcagg cagttccgag gatctgtact ttcagagcga taacgatgga tccgaaatcg
     8821 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
     8881 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
     8941 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
     9001 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
     9061 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
     9121 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
     9181 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
     9241 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
     9301 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     9361 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
     9421 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
     9481 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
     9541 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
     9601 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     9661 agatcgcgcg ctggctgtct actctggaga tttccggtta aggccgcact cctcagctag
     9721 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     9781 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     9841 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     9901 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     9961 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    10021 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    10081 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
    10141 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    10201 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    10261 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
    10321 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
    10381 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
    10441 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
    10501 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    10561 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
    10621 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
    10681 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
    10741 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
    10801 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
    10861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
    11041 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    11101 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    11161 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    11221 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    11281 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    11341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    11401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    11461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    11521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    11581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    11641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11701 gggatt
//
