LOCUS       sequence-355790-       10734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of plasma membrane-targeted Halo tagged mouse 
            Syt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag
FEATURES             Location/Qualifiers
     source          1..10734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..70)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        55..384
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      235..370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     297..316
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        432..479
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             498..872
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1002..1123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1039..1058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1093..1112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1172..1188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1172..1188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1185..1207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1196..1212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1220..1226,1227..1244,1245..1250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1265..1286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1403..1420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1574..2162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1654..1673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2333..3124,3125..3193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2956..2975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3194..3298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3373..3392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3564..3943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3945..4143
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4098..4118
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4161..4341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4388..4513
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5006..5239
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5424..5468
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5766..5883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5928..6374
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             7744..7764
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             7780..8664
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    8752..9340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8805..8825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9223..9234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9343..9359)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9344..9360)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9865..10045
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10071..10088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10077..10301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10347..10734,1..41)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10434..10453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10644..10665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
       61 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      301 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      361 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      421 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
      481 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
      541 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
      601 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
      661 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
      721 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
      781 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
      841 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
      901 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
      961 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     1021 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1081 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     1141 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3301 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     3361 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     3421 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     3481 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     3541 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     3601 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3661 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3721 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3781 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3841 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3901 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3961 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4021 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     4081 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4141 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4321 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     4381 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     4441 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     4501 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     4561 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     4621 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     4681 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     4741 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     4801 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     4861 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     4921 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     4981 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     5041 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     5101 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     5161 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     5221 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     5281 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     5341 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     5401 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     5461 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     5521 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     5581 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     5641 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     5701 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     5761 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     5821 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     5881 tttcgggttt attacaggga cagcagagat ccagtttggt taattaaagt gcaagtggtt
     5941 ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg
     6001 acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa
     6061 acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc
     6121 ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg
     6181 ccggtccccc gtaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc
     6241 cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct
     6301 gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt
     6361 gcctgagagc gcaggtacgg ctagcggatc gccaccatga agctctccct ggtggccgcg
     6421 atgctgctgc tgctcagcgc ggcgcgggcc agcggtggcg gcggaagtgg aggtggaggc
     6481 tcggttctgc ccacatggtc caccccggtg cagccaatgg ccctgattgt gctggggggc
     6541 gtcgccggcc tcctgctttt cattgggcta ggcatcttct tcaagtacct gcagaaaaga
     6601 agggagcgga gacgccagcg caaactgggc aaacgctaca agaattcctt ggagacggtg
     6661 ggcacgccag actcggggcg tgggcgcggt gagaagaaag ccatcaagtt gcctgcagga
     6721 gggaaggctg tgaatacagc cccagtgccc ggccagacgc cacacgatga gtctgaccgc
     6781 agaacggaga cccgttcctc tgtctcggac ctcgtcaact cccttaccag cgagatgctc
     6841 atgctctccc cgggttctga ggaggatgag gcccacgagg gctgcagccg agagaacctg
     6901 ggccgaatcc agttcagtgt tggctacaac ttccaagagt ccacactcac cgtgaaggtc
     6961 atgaaggccc aagagctgcc agccaaggac ttcagcggta ctagtgaccc ctttgtcaag
     7021 atctacctgc tacccgacaa gaagcacaaa ctggagacca aggtgaagcg gaagaatcta
     7081 aacccgcact ggaatgagac ctttctattt gaagggtttc cctacgagaa agtggtgcag
     7141 agggtcctct acctccaggt cctggattat gaccgtttca gccgcaatga ccccattggg
     7201 gaggtgtcca tccctctgaa caaggtggac ctgacccaga tgcagacctt ctggaaggat
     7261 ctgaagccat gcagcgatgg gagtgggagc cgaggggagc tgctcttgtc cctctgctac
     7321 aacccctctg ccaactccat catcgtgaac atcatcaaag ctcgaaacct caaagccatg
     7381 gacatcgggg gcacatcaga cccctatgtg aaggtgtggc tgatgtataa agacaagcgg
     7441 gtagagaaaa agaagaccgt gacaaagaag aggaacctga accccatctt caatgagtct
     7501 ttcgccttcg acatacccac ggagaagctg agggagacca cgatcatcat cactgtcatg
     7561 gacaaagaca agctcagccg caacgacgtc atcggcaaga tctacctgtc ctggaagagc
     7621 ggaccaggtg aagtgaaaca ctggaaggac atgatcgctc gtccccggca gcctgtggcc
     7681 cagtggcacc agctgaaagc cggatccgga agttcggggt ccagcggttc ttcaggcagt
     7741 tccgaggatc tgtactttca gagcgataac gatggatccg aaatcggtac tggctttcca
     7801 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
     7861 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
     7921 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg
     7981 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
     8041 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
     8101 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
     8161 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
     8221 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
     8281 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
     8341 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
     8401 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
     8461 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
     8521 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg
     8581 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
     8641 ctgtctactc tggagatttc cggtgagaac gcaaacagct tttgctatga aaatgaagtt
     8701 gccctctaag gccgcactcc tcagctagaa ttcgatatca agcttatcga taatcaacct
     8761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9121 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     9181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9301 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     9361 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     9421 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     9481 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     9541 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     9601 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     9661 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     9721 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     9781 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     9841 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     9901 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9961 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10021 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
    10081 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    10141 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    10201 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    10261 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    10321 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    10381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attg
//
