LOCUS       sequence-355791-       11451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse
            Syt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
FEATURES             Location/Qualifiers
     source          1..11451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        18..347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     260..279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        395..442
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             461..835
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    965..1086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1002..1021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1056..1075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1135..1151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1135..1151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1148..1170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1159..1175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1183..1189,1190..1207,1208..1213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1228..1249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1366..1383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1537..2125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1617..1636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2296..3087,3088..3156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2919..2938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3157..3261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3336..3355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3527..3906
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3908..4106
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4061..4081
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4124..4304
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4351..4476
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4969..5202
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5387..5431
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5729..5846
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5891..6337
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      6369..6378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8466..8486
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             8502..9386
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    9432..10020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9485..9505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9903..9914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10023..10039)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10024..10040)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10545..10725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10751..10768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10757..10981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11027..11451,1..4)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11114..11133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11324..11345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      361 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
      421 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
      481 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
      541 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
      601 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
      661 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
      721 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
      781 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
      841 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
      901 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
      961 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1081 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     1141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1201 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     3301 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3361 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     3421 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     3481 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     3541 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     3601 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3661 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3721 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3781 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3841 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3901 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     3961 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4021 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     4081 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     4141 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4201 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4261 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     4321 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     4381 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     4441 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     4501 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     4561 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     4621 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     4681 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     4741 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     4801 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     4861 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     4921 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     4981 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     5041 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     5101 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     5161 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     5221 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     5281 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     5341 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     5401 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     5461 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     5521 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     5581 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     5641 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
     5701 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     5761 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     5821 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     5881 ggttaattaa agtgcaagtg gttttaggac caggatgagg cggggtgggg gtgcctacct
     5941 gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc
     6001 cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc
     6061 agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac
     6121 tgaaggcgcg ctgacgtcac tcgccggtcc cccgtaaact ccccttcccg gccaccttgg
     6181 tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg
     6241 gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt
     6301 gggcagcgga ggagtcgtgt cgtgcctgag agcgcaggta cggctagcgg atccggtacc
     6361 gcgggcccgc caccatggac gtggtgaatc agctggtggc tgggggtcag ttccgggtgg
     6421 tcaaggagcc ccttggcttc gtgaaggtgc tgcagtgggt ctttgccatc ttcgcctttg
     6481 ctacgtgtgg cagctacacc ggggagcttc ggctgagcgt ggagtgtgcc aacaagacgg
     6541 agagtgccct caacatcgaa gttgaattcg agtacccctt caggctgcac caagtgtact
     6601 ttgatgcacc ctcctgcgtc aaagggggca ctaccaagat cttcctggtt ggggactact
     6661 cctcgtcggc tgaattcttt gtcaccgtgg ctgtgtttgc cttcctctac tccatggggg
     6721 ccctggccac ctacatcttc ctgcagaaca agtaccgaga gaacaacaaa gggcctatga
     6781 tggactttct ggctacagcc gtgttcgctt tcatgtggct agttagttca tcagcctggg
     6841 ccaaaggcct gtccgatgtg aagatggcca cggacccaga gaacattatc aaggagatgc
     6901 ccatgtgccg ccagacaggg aacacatgca aggaactgag ggaccctgtg acttcaggac
     6961 tcaacacctc agtggtgttt ggcttcctga acctggtgct ctgggttggc aacttatggt
     7021 tcgtgttcaa ggagacaggc tgggcagccc cattcatgcg cgcacctcca ggcgccccgg
     7081 aaaagcaacc agcacctggc gatgcctacg gcgatgcggg ctacgggcag ggccccggag
     7141 gctatgggcc ccaggactcc tacgggcctc agggtggtta tcaacccgat tacgggcagc
     7201 cagccagcgg tggcggtggc tacgggcctc agggcgacta tgggcagcaa ggctatggcc
     7261 aacagggtgc gcccacctcc ttctccaatc agatgaaagg gtcaggttct tcgggcagtt
     7321 cggggtccag cggatctagt cgcaaactgg gcaaacgcta caagaattcc ttggagacgg
     7381 tgggcacgcc agactcgggg cgtgggcgcg gtgagaagaa agccatcaag ttgcctgcag
     7441 gagggaaggc tgtgaataca gccccagtgc ccggccagac gccacacgat gagtctgacc
     7501 gcagaacgga gacccgttcc tctgtctcgg acctcgtcaa ctcccttacc agcgagatgc
     7561 tcatgctctc cccgggttct gaggaggatg aggcccacga gggctgcagc cgagagaacc
     7621 tgggccgaat ccagttcagt gttggctaca acttccaaga gtccacactc accgtgaagg
     7681 tcatgaaggc ccaagagctg ccagccaagg acttcagcgg tactagtgac ccctttgtca
     7741 agatctacct gctacccgac aagaagcaca aactggagac caaggtgaag cggaagaatc
     7801 taaacccgca ctggaatgag acctttctat ttgaagggtt tccctacgag aaagtggtgc
     7861 agagggtcct ctacctccag gtcctggatt atgaccgttt cagccgcaat gaccccattg
     7921 gggaggtgtc catccctctg aacaaggtgg acctgaccca gatgcagacc ttctggaagg
     7981 atctgaagcc atgcagcgat gggagtggga gccgagggga gctgctcttg tccctctgct
     8041 acaacccctc tgccaactcc atcatcgtga acatcatcaa agctcgaaac ctcaaagcca
     8101 tggacatcgg gggcacatca gacccctatg tgaaggtgtg gctgatgtat aaagacaagc
     8161 gggtagagaa aaagaagacc gtgacaaaga agaggaacct gaaccccatc ttcaatgagt
     8221 ctttcgcctt cgacataccc acggagaagc tgagggagac cacgatcatc atcactgtca
     8281 tggacaaaga caagctcagc cgcaacgacg tcatcggcaa gatctacctg tcctggaaga
     8341 gcggaccagg tgaagtgaaa cactggaagg acatgatcgc tcgtccccgg cagcctgtgg
     8401 cccagtggca ccagctgaaa gccggatccg gaagttcggg gtccagcggt tcttcaggca
     8461 gttccgagga tctgtacttt cagagcgata acgatggatc cgaaatcggt actggctttc
     8521 cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc
     8581 cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc
     8641 gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta
     8701 tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg
     8761 atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct
     8821 ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta
     8881 tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc cgcgagacct
     8941 tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag aacgttttta
     9001 tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt
     9061 accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc
     9121 tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc
     9181 tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac
     9241 cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc
     9301 cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct
     9361 ggctgtctac tctggagatt tccggttaag gccgcactcc tcagctagaa ttcgatatca
     9421 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     9481 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     9541 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     9601 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     9661 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     9721 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     9781 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     9841 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     9901 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     9961 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10021 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
    10081 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
    10141 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10201 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
    10261 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
    10321 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    10381 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
    10441 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
    10501 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
    10561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
    10741 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    10801 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    10861 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    10921 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
    10981 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
    11041 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    11101 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11161 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11221 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    11281 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    11341 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    11401 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc g
//
