LOCUS       sequence-355794-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of synaptic vesicle-targeted mouse Syt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
  TITLE     Synaptotagmin 7 is targeted to the axonal plasma membrane through 
            gamma-secretase processing to promote synaptic vesicle docking in 
            mouse hippocampal neurons.
  JOURNAL   Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
  PUBMED    34543184
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-09-20"; volume: "10. pii: 67261. doi"; pages: " 
            10.7554/eLife.67261"
COMMENT     Sequence Label: pF(UG) hSyn SYP-deltaTMD SYT7alpha
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(295..315)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        300..629
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      480..615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     542..561
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        677..724
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             743..1117
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1247..1368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1284..1303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1338..1357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1417..1433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1417..1433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1430..1452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1441..1457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1465..1471,1472..1489,1490..1495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1510..1531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1648..1665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1819..2407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1899..1918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2578..3369,3370..3438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3201..3220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3439..3543)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3618..3637)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3809..4188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4190..4388
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4343..4363
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4406..4586
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4633..4758
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5251..5484
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5669..5713
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6011..6128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6173..6619
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      6651..6660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8760..9348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8813..8833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9231..9242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9351..9367)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9352..9368)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9873..10053
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10079..10096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10085..10309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10355..10497,1..286)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10442..10461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
       61 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      301 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      361 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      421 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      481 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      541 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
      601 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
      661 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
      721 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
      781 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
      841 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
      901 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
      961 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     1021 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     1081 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     1141 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     1201 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     1261 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1321 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     1381 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1501 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1561 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1621 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1741 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1801 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1861 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1981 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2221 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2281 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2341 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2401 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2461 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3421 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3481 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3541 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     3601 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     3661 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     3721 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     3781 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     3841 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     3901 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3961 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4021 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4081 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4141 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     4201 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     4261 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     4321 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     4381 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     4441 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4501 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4561 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     4621 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     4681 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     4741 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     4801 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     4861 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     4921 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     4981 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     5041 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     5101 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     5161 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5221 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5281 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5341 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5401 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5461 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     5521 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     5581 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     5641 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     5701 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     5761 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     5821 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     5881 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     5941 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     6001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     6061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     6121 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt aaagtgcaag
     6181 tggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     6241 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     6301 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     6361 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     6421 actcgccggt cccccgtaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     6481 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     6541 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     6601 gtcgtgcctg agagcgcagg tacggctagc ggatccggta ccgcgggccc gccaccatgg
     6661 acgtggtgaa tcagctggtg gctgggggtc agttccgggt ggtcaaggag ccccttggct
     6721 tcgtgaaggt gctgcagtgg gtctttgcca tcttcgcctt tgctacgtgt ggcagctaca
     6781 ccggggagct tcggctgagc gtggagtgtg ccaacaagac ggagagtgcc ctcaacatcg
     6841 aagttgaatt cgagtacccc ttcaggctgc accaagtgta ctttgatgca ccctcctgcg
     6901 tcaaaggggg cactaccaag atcttcctgg ttggggacta ctcctcgtcg gctgaattct
     6961 ttgtcaccgt ggctgtgttt gccttcctct actccatggg ggccctggcc acctacatct
     7021 tcctgcagaa caagtaccga gagaacaaca aagggcctat gatggacttt ctggctacag
     7081 ccgtgttcgc tttcatgtgg ctagttagtt catcagcctg ggccaaaggc ctgtccgatg
     7141 tgaagatggc cacggaccca gagaacatta tcaaggagat gcccatgtgc cgccagacag
     7201 ggaacacatg caaggaactg agggaccctg tgacttcagg actcaacacc tcagtggtgt
     7261 ttggcttcct gaacctggtg ctctgggttg gcaacttatg gttcgtgttc aaggagacag
     7321 gctgggcagc cccattcatg cgcgcacctc caggcgcccc ggaaaagcaa ccagcacctg
     7381 gcgatgccta cggcgatgcg ggctacgggc agggccccgg aggctatggg ccccaggact
     7441 cctacgggcc tcagggtggt tatcaacccg attacgggca gccagccagc ggtggcggtg
     7501 gctacgggcc tcagggcgac tatgggcagc aaggctatgg ccaacagggt gcgcccacct
     7561 ccttctccaa tcagatgaaa gggtcaggtt cttcgggcag ttcggggtcc agcggttctt
     7621 caggcagttc ccgcaaactg ggcaaacgct acaagaattc cttggagacg gtgggcacgc
     7681 cagactcggg gcgtgggcgc ggtgagaaga aagccatcaa gttgcctgca ggagggaagg
     7741 ctgtgaatac agccccagtg cccggccaga cgccacacga tgagtctgac cgcagaacgg
     7801 agacccgttc ctctgtctcg gacctcgtca actcccttac cagcgagatg ctcatgctct
     7861 ccccgggttc tgaggaggat gaggcccacg agggctgcag ccgagagaac ctgggccgaa
     7921 tccagttcag tgttggctac aacttccaag agtccacact caccgtgaag gtcatgaagg
     7981 cccaagagct gccagccaag gacttcagcg gtactagtga cccctttgtc aagatctacc
     8041 tgctacccga caagaagcac aaactggaga ccaaggtgaa gcggaagaat ctaaacccgc
     8101 actggaatga gacctttcta tttgaagggt ttccctacga gaaagtggtg cagagggtcc
     8161 tctacctcca ggtcctggat tatgaccgtt tcagccgcaa tgaccccatt ggggaggtgt
     8221 ccatccctct gaacaaggtg gacctgaccc agatgcagac cttctggaag gatctgaagc
     8281 catgcagcga tgggagtggg agccgagggg agctgctctt gtccctctgc tacaacccct
     8341 ctgccaactc catcatcgtg aacatcatca aagctcgaaa cctcaaagcc atggacatcg
     8401 ggggcacatc agacccctat gtgaaggtgt ggctgatgta taaagacaag cgggtagaga
     8461 aaaagaagac cgtgacaaag aagaggaacc tgaaccccat cttcaatgag tctttcgcct
     8521 tcgacatacc cacggagaag ctgagggaga ccacgatcat catcactgtc atggacaaag
     8581 acaagctcag ccgcaacgac gtcatcggca agatctacct gtcctggaag agcggaccag
     8641 gtgaagtgaa acactggaag gacatgatcg ctcgtccccg gcagcctgtg gcccagtggc
     8701 accagctgaa agcctaaggc cgcactcctc agctagaatt cgatatcaag cttatcgata
     8761 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8821 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8881 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8941 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     9001 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     9061 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     9121 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     9181 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9241 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9301 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     9361 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     9421 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     9481 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9541 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     9601 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     9661 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     9721 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     9781 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     9841 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     9901 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9961 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10021 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
    10081 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
    10141 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
    10201 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
    10261 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
    10321 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
    10381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    10441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaa
//
