LOCUS       sequence-355796-        3042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing HES5 transcription factor, part of the Human
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HES5
FEATURES             Location/Qualifiers
     source          1..3042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(599..698)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(739..755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(739..755)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(857..876)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             868..1677
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(935..954)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1824..2412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2313..2332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2566..2583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2742..2769)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2861..2947)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2996..3018
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3010..3027
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3011..3027
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctcccag cactgtggcc
      121 gtggagctgc tcagccccaa agagaaaaac cgactgcgga agccggtggt ggagaaaatg
      181 cgccgcgacc gcatcaacag cagcatcgag cagctgaaac tgctgctaga gcaggagttc
      241 gcgaggcacc agcccaactc caagctggag aaggccgaca tcttggagat ggctgtcagc
      301 tacctgaagc acagcaaagc cttcgtcgcc gccgcaggcc ctaagagcct acaccaggat
      361 tacagcgaag gctactcgtg gtgcttgcag gaggccgtgc aattcctgac gctacacgcc
      421 gctagtgaca cgcagatgaa gctgctgtac cacttccagc gtcctcctgc cgctccagct
      481 gcacccgcta aagagcccaa agctcctggt gcagcacctc caccagctct ttcagcaaag
      541 gcaacagctg ccgcagctgc tgctcatcag cctgcatgcg gcctatggcg tccttggtgc
      601 ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      661 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      721 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      781 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
      841 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
      901 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      961 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1021 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1081 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1141 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1201 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1261 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1321 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1381 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1441 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1501 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1561 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1621 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1681 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1741 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     1801 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1861 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1921 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1981 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2041 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2101 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2161 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2221 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2281 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2341 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2401 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2461 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2521 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2581 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     2761 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     2821 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     2881 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     2941 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3001 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
