LOCUS       sequence-35580-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNI9
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     786..805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
       61 aaaaatatat catcatgcct cctctagagg tctcctcgaa acggtgcagc ggctgttgcc
      121 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat
      181 tggcggcaag caagcgctcg gagaccctcg agccacccat gaccaaaatc ccttaacgtg
      241 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      841 cgtcgatttt tgtgatgctc g
//
