LOCUS       sequence-355829-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic coupling to dimeric moss kinesin-14 for retrograde 
            transport via SSPB(micro).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        403..678
                     /label=chicken beta-actin promoter
     primer_bind     1621..1643
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1706..1715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1745..1951,1952..1954,1955..2074)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             join(2183..2185,2186..2896)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2183..2185,2186..2896)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2225..2246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2486..2505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2833..2854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             3334..3342
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4547..4681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4584..4603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4638..4657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4695..4711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4695..4711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4708..4730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4719..4735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4743..4749,4750..4767,4768..4773))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4788..4809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4926..4943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5097..5685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5177..5196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5856..6647,6648..6716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6479..6498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6717..6821)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6889..6907
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6945..6967)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7067..7086
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7280..7302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7294..7311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7295..7311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccctcggtgt tattgctgct ctgtgcacgc acgctcattg tatcacttca tcccagtgca
       61 tgcactcaca ctggagcgat tgctgctcgg tgcacgcaca ctcattgtat cactgcagct
      121 cagtggctgc acgcacaccg gtgttattgc tgctcggtgc gtgcatgcac atcagtgtcg
      181 ctgcagctca gtgcatgcac gctcattgcc catcgctatc cctgcctctc ctgctggcgc
      241 tccccgggag gtgacttcaa ggggaccgca ggaccacctc gggggtgggg ggagggctgc
      301 acacgcggac cccgctcccc ctccccaaca aagcactgtg gaatcaaaaa ggggggaggg
      361 gggatggagg ggcgcgtcac acccccgccc cacaccctca cctcgaggtg agccccacgt
      421 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
      481 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggsggggcg
      541 gggsggggsg rggggsgggg sggggcgagg cggagaggtg cggcggcagc caatcagagc
      601 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      661 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      721 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      781 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct
      841 tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag
      901 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
      961 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     1021 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     1081 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg
     1141 gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     1201 cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     1261 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     1321 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     1381 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     1441 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     1501 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc
     1561 tccagcctcg gggctgtccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg
     1621 ggttcggctt ctggcgtgtg accggcgggg tttatatctt cccttctctg ttcctccgca
     1681 gcccccaagc taggcaattg ggcgcgccac catggatctg agcggcctga ccctgcagga
     1741 attcagctcc ccgaaacgcc ctaagctgct gcgtgaatat tacgattggc tggttgataa
     1801 cagctttacc ccatatctgg tggtggatgc cacatacctg ggcgtgaacg tgcccgtgga
     1861 gtatgtgaaa gacggtcaga tcgtgctgaa tctgtctgca agtgcgaccg gcaacctgca
     1921 actgacaaat gattttatcc agttcaacgc ccagtttaag ggcgtgtctc gtgaactgta
     1981 tatcccgatg ggtgccgctc tggccattta cgctcgcgag aacggcgatg gtgtgatgtt
     2041 cgaaccagaa gaaatctatg acgagctgaa tattggtcaa ttgtctagag gtggcggggg
     2101 cagtggggga gggggaagtg gaggcggcgg ttctggcggg ggaggctccg gkggcggggg
     2161 grgcgggggg gggagtaagc ttgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2221 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2281 cgagggcgat gccacctacg gcaagctgac cctgaagttg atctgcacca ccggcaagct
     2341 gcccgtgccc tggcccaccc tcgtgaccac cctcggctac ggcctgcagt gcttcgcccg
     2401 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2461 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2521 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2581 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2641 ggccgacaag cagaagaacg gcatcaaggc aaacttcaag atccgccaca acatcgagga
     2701 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2761 gctgctgccc gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga
     2821 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2881 ggacgagctg tacaaggcta gcggaggcgg tggctcaggc ggaggagggt caggaggggg
     2941 aggcagtggc gggggcggct ctggaggagg gggttctggt ggtggtagct acgtaagaga
     3001 tgatttgagg ggacgacttg taactctcga gaagaaatta gcagagcgta cccaagattt
     3061 gagtcttgca gagtctactt tggcaactcg tgagggtgaa ttcgaatccc tgcaaagcaa
     3121 tctaatggag cttaatgagc tccgggagat gaaggaggat atcgacagaa agaatgagca
     3181 aacagcagct atattgaagc gacaagcaga ccagataact gaattagaaa ctctttatag
     3241 ggaagaacaa gtccttcgaa aacgatactt caacatgatg gaagacatga aaggtaagat
     3301 cagggtatat gctcggtgga gacccctaag tagtaaggag gtcaaagagc ggcaacaaaa
     3361 cgtgttgata gcacctgatg aattcaccat cgaacatcct tggaaagacg acaaacccaa
     3421 acaacatcaa tttgatcacg ttttcgacca ccatgcgacc caagaagagg tctttgagga
     3481 tacaaagtat ttggtccaat cagcaattga cggatacaat gtctgtatat ttgcatacgg
     3541 gcaaactggg tcaggcaaaa cgtttacgat ttacggttct gacaacaacc caggtctaac
     3601 tccacgagct accaaagaac tttttggcta tcttaagcga gacgccaata agttttcgtt
     3661 tgctctgaag gtttacatgc ttgaaatata tcaagattcg ctcatcgatc tgcttctacc
     3721 aaagagtgct gcaaagccac gaaagcttga gattaagaag gactccaagg gaatggttgt
     3781 cgtagagaat gcaactctac tgccgattgc atcccatgat gaactgcaag ccattgttca
     3841 taagggtctg gagagacgac atgtttcggg cacccacatg aatgcggaga gttcacggtc
     3901 acacctcatt ctatcagtaa tcgttgaaag caccaaccga cagtctcaag ttcttgtgaa
     3961 gggcaagctg agttttgtgg atcttgcggg ctctgaaaga gtgaagaaat ctggatccag
     4021 tggtgaacaa ctgaaagagg cccaaagcat taataaatct ttgtcagcac taggtgatgt
     4081 cattagcgcc cttgcaacag aggagcagca tattccttac cgtaaccaca agctaacaat
     4141 gcttatgagc gattctctcg gtggaaatgc gaagaccctc atgtttgtaa atatctcacc
     4201 agcagagtct aatctggacg agacccataa ttcactctgc tatgccacaa gggtaagatc
     4261 catcatcaat gaggccagta aaaatactac aacgaaagag atactgcgac tgaagaaaca
     4321 aatcgcgtac tggaaggagc aagcgggcag gatgggaggg tccgaggatc tggaagaggt
     4381 tatggatgat cgtggaatca aaactgcaga ttaaacgcgt accggtccta gataagtaat
     4441 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
     4501 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     4561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     4681 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6841 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6901 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6961 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7021 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     7081 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7141 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7201 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     7261 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg
     7321 ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc
     7381 cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct
     7441 accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac
     7501 cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg
     7561 gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg
     7621 gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta
     7681 cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt
     7741 caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc
     7801 cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc
     7861 ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac
     7921 agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg
     7981 gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc
     8041 ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg
     8101 catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca
     8161 aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg
     8221 cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc
     8281 ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc
     8341 cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg
     8401 aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac
     8461 gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg
     8521 tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc
     8581 tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg
     8641 catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg
     8701 catgcatac
//
