LOCUS       sequence-35585-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNI4
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..17)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     778..797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
       61 caagataaaa atatatcatc atgcctcctc tagaggtctc gccaggacca tggcctgacc
      121 ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg
      181 cagcggctgt tggagaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
      241 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      841 tttgtgatgc tcgtcagggg ggcggagcct
//
