LOCUS       sequence-35586-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNI3
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..20)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4..20)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     794..813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
       61 gcacaagata aaaatatatc atcatgcctc ctctagaggt ctcaggacca tggcctgacc
      121 ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg
      181 cagcggctgt tgccggtgct gtgccaggga gaccctcgag ccacccatga ccaaaatccc
      241 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcc
//
