LOCUS       sequence-355860-        8042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R
  TITLE     The E6 and E7 genes of the human papillomavirus type 16 together are
            necessary and sufficient for transformation of primary human 
            keratinocytes.
  JOURNAL   J Virol. 1989 Oct . 63(10):4417-21.
  PUBMED    2476573
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 Oct . 63(10):4417-21."
COMMENT     Sequence Label: pB-actin
FEATURES             Location/Qualifiers
     source          1..8042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4686..4704)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4772..4876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4877..4945,4946..5737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5095..5114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5908..6496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6397..6416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6650..6667
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaagcaatgc tatcacctcc cctgtgtgga cttgggagag gactgggcca ttctccttag
       61 agagaagtgg ggtggctttt aggatggcaa gggacttcct gtaacaatgc atctcatatt
      121 tggaatgact attaaaaaaa caacaatgtg caatcaaagt cctcggccac attgtgaact
      181 ttgggggatg ctcgctccaa ccgactgctg tcaccttcac cgttccagtt tttaaatcct
      241 gagtagctta tcgatgataa gctgtcaaac atgagaattc caagcttggg ctgcaggtcg
      301 actgatcctg cgagaatagc cgggcgcgct gtgagccgaa gtcgcccccg ccctggccac
      361 ttccggcgcg ccgagtcctt aggccgccag ggggcgccgg cgcgcgccca gattggggac
      421 aaaggaagcc gggccggccg cgttattacc ataaaaggca aacactggtc ggaggcgtcc
      481 ccgcggcgcg cggcaggaag ccaggcccca accccctccc aaccgggcgc cagccccgcc
      541 tccgcccggt tcaaacagcg ccgggtcggc gcgcgcgcac gcagcggcca caccctcggg
      601 cggccagcgg ctcgggcagg aagtgcgcgc aagcgcccgg gagccgcggc gaccccaccc
      661 cttccggccg agcccgcctt cgccccagcc caggccgcgg caccccgggc cccagaacgc
      721 acgcgcaatt agcgccttga gtcccagcgc gcacgcgcaa ttagcgccaa ttcccagcgc
      781 gcacgcagtt agcgcccaaa ggaccagcgc gcacgcgcaa tggcgcccca gcccccaccg
      841 ggcctggcgg gggctacgcc gcgcccaccc tgcgatcccc attggcaaga gcccggctca
      901 gacaaagacc ccgccggttg cccccgcccc gagagcggca cccccggagc gcgcctcccg
      961 agcgcggcct cgcgcctccg aactggcgtg gggtgtcccc catctccgga ggcccagggg
     1021 cttctcccgc gccccccacg gcggtccggt tcccccccca tgcgcccccc gctgcggccc
     1081 agacggcggc tctgcacggg cgaaggggcc gcggccgcat gccccggtcg gctggccggg
     1141 cttacctggc ggcgggtgtg gacgggcggc ggatcggcaa aggcgaggct ctgtgctcgc
     1201 ggggcggacg cggtctcggc ggtggtggcg cgtcgcgccg ctgggtttta tagggcgccg
     1261 ccgcggccgc tcgagccata aaaggcaact ttcggaacgg cgcacgctga ttggccccgc
     1321 gccgctcact caccggcttc gccgcacagt gcagcatttt tttaccccct ctcccctcct
     1381 tttgcgaaaa aaaaaaagag cgagagcgag attgaggaag aggaggaggg agagttttgg
     1441 cgttggccgc cttggggtgc tgggcccggg ggctgggggc gcgcgccgtg gcccccgcgc
     1501 cccacgctgg gcagtgcccg gttcggcccc gcatggccag gcctgccccc ggcctgcccg
     1561 tctctcgggc cccccaccca ccgcgggaca tcctaggtgt ggacatctct tgggcactga
     1621 gcgcccaggt ggggtgggcc agggtctgca cgggtgccag ggccctgggt tctgtacgct
     1681 cctgcagaag gagctcttgg agggcatgga gtggccaggc agtcactccc ccttgccgac
     1741 ttcagagcaa ctgccctgaa agcagggcct gaggacctct ggctgtgggg ctcagctagc
     1801 taaatgtgct gggtgggtca ctagggagag acctgggctt gagaggtaga gtgtggtgtt
     1861 gggggagtca ggtggcttgc ggccacttag aagtcgcagg accacactcc ccaggacagg
     1921 gcaggggcca gcggtccagt ggctggaggt ggcccgtgat gaaggctaca aacctaccca
     1981 gccgcagccc tgggaaggaa ggtgggctct acagggcagg gcacctttta ccctggagct
     2041 gcctgctttt gagggtaaca gtcacgccca gccaagacca ggcctggggc gttagtgggt
     2101 gacctaggca ctgcggggcg ggggggctgg gtctacacag cctgggtctg ggcccaccgt
     2161 ccgttgtatg tctgctatgc gcagccacag ctgaactgcc ctcccagacc atctggaggc
     2221 cgctggggga ctctggggac caagactcca tgtgccacag aggattgggg gcggggcggt
     2281 gctaggaact caaagccagc ctgggaagac cctgtccttg tcaccctttc ttgccttggg
     2341 tctgtccact gagtagcaca caagaccggg tgggcagggt ccgttctgct ccgggaatca
     2401 cagactgtgt gtacccaggt ggtgggcatg cagcgatcag tggcgtggga ccacagaggg
     2461 gcattcggct cagcctagag gaacctgcct tcctcctcct ccccctcctt ggaggtggtg
     2521 acaagaagct ggatttttac acaaaaatag gaagagtggt gaggtggggg cacctcaggc
     2581 ctgcggggct ctttgcagcg cgtagtaggt gttcattgag tgtgtgtcgc aataggcaaa
     2641 gatgagtcca tataaggaca tggaacctgt ggccaggaag gaggctgctt gttccagttc
     2701 acggtacaag gctgtgctca gccaatggga cctgctcctc ccttgaaggt tgcagaggcc
     2761 acagcctggt gggaaagatg accaccaccc agcacacagt ggcagacaca ggttcacagt
     2821 ccagaagact tgctgagcct cctccatcac caccccacgc aagggcaaac ttccccagcc
     2881 tgtatactcc ctgcctcctg gggatgggac tgtcctgtcc ctggccttgt atctttcagc
     2941 tgcaggcgtc ttgccagctg cctcaggacc tgccaacgct acagttcacc cggctggcgt
     3001 tgtggggcga tgggcggggg gcccttcttg ctgcctgttg atgagggctg gttctggggg
     3061 ttcgtggaac gttgaactgg gtggagtgct gggggggtgg ggtgggcagt ggggatggga
     3121 ccggatctac agagcaggga tgagaggttg tcaggtgggc ggctccaggc agcaaggtgg
     3181 ggtgcagagc agacctgatc cccaggcagg acctgtgttg ccgctgggcc tccattcagc
     3241 tggccaagag acaggagctc cagccccacc ccgccccggg ctgcagcatt cctggcctgt
     3301 cctcctggct tgtggttccc tctgtctgca gcggccactt cctagtgctc tcctatgcac
     3361 ccttcaagtc ccatcccaga aagcccctct ttggggaagc gttttagcct ctgcagccac
     3421 gccttcctcc ctacctcaga ccaaggcttg cagctggcat ttagaactgg gcctcagcca
     3481 ccaggctgcg agccctccca gggtctccgt cacccccact ccttcccact acacccaccc
     3541 cagggctcct cgctgttgaa tgagtgactg cctgggtgtc ctcaggtggc aggttggagg
     3601 tggccgagca gcctggggtg aggggcaggg aggagctgaa gagagaaagc aagcagcgga
     3661 gcaggaaaag aggaagtaga ggtgcctggc cagggggcgg gccgagcagg ggagaagtga
     3721 acaggttctc ttctgtgctt gggaactttg ccctgtaagg gagctttcgg agctgtcact
     3781 ggggcacgca cgcagccccc ggagaaccct cggttgaact cagccaaggg gactccacct
     3841 gcctggctgg gacttttgtt ttttcccctc caagcctttg ctttggtgac acccccaccc
     3901 tgcaaggcct ccaggcaacc aactcttaca gcgcagaccc agggaccact ccttggccct
     3961 cccgcaaaac aaccacccag ccaccttgcc ctgggcactg cagcgccctc cctatcctgc
     4021 caggagggaa tccaacctcc tttttattat tattactatt tttgatacaa ggtcttgctg
     4081 tgatgcccag gctggagtgc gatcacagct cactgcagcc tcgacctcct gggctcaaag
     4141 ccttcacgag tagctgggac tataggcaca cacttccaag cctggctaat ttttaaatta
     4201 tctgtagagg tatggcttct caccatgttg cccaggctgg tctccagctc cggggggctc
     4261 aagcgatcct cccacctcag cctcccaaag tcctgggatt acaggcatga gccaccgtgc
     4321 ccgtcctcaa gctccatttt gtagaggaag gaactgagac agagcttccc tccaagactt
     4381 gagttagctc taggaggaca gggaccctga acacggtagg tgctcagtga gaggagaatg
     4441 aatgaatgag ggggggcaac attccaggag acacaccccc attcacagac taggaaactg
     4501 agtcaggact ggtgaagtgc tcaagtcccc aaatgcggtg ggaaggactc taggctcagg
     4561 gtccctgagg cggcagccct gcctcctgcc tcacactgcc cctccctggt aaagggtccc
     4621 ctggaatgtc cccaaggcct gagtccaaag gctgtttgaa agtcggaatt cgatgataag
     4681 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5101 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5401 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     5461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6601 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6661 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6721 ttcacaccgc atagatcctc accccctttt cttccttcat tctctgctgg tgtcaatcac
     6781 agcttctagc agagggaagg caggccaggc ttggtttctt cttgcgagag tatttaactc
     6841 agggccctgt agaacaatga gaatctgacc tgcaactagc tgggcgtgct ggggcatgcc
     6901 tgtgtagttt cagctacttg ggaggctgag gcaggagaat tgcttgagcc caaagttgag
     6961 gctgcagtga gccatggttg tgccattaca ctccagcctg ggcaacacaa gaccccgtct
     7021 cagaaataaa aagagaacct ggcctgcagt gccaggcagg ccctgaggtc caggagcctg
     7081 ggtatctccc tctgcagcat gggtcacgaa caaactgggc cctcagaggc cacgggatgg
     7141 cgcccagtct ccagtcacaa ggcagaatcc agacctcagc ccatagctaa ccagagctgt
     7201 ctgcaggcca gatatggccc catggacccc ccaccccaac ttgactttga ttccaggtcc
     7261 ccctctgtct ggatgaacag gtaggaattg cggcctctcc taagcctatg ggagcctggg
     7321 ccaggctgac cctcaaggct gacagccacg atcccatagg tgaaggcaaa ggcgtggcag
     7381 ctaggaacac tcactacacc cccactcaag ggaacaccgt gcccagctac cctgagacag
     7441 ccccactccc aggaaatgca ggtgccaacc agccccagtg aggcatggca cctgagccag
     7501 acacccccaa atagtccctt cccacctcct caagcatctg cactctgggt gaacccagaa
     7561 aaactggggt acctggggag acatagaagg gccaggccag gaactcccca ataagcagga
     7621 acagagacct gacccctgag cctcccccac cctctaaggc tgctcaatgt caaggcagga
     7681 gggatctcca cacacctgca ctctgggtga ggacaagttg gcccccactg ccccaccctt
     7741 ttgcagggtt caccctcctg cagggttcac cctctgctgc ccccaaccca gccacaccac
     7801 aaagtcacac ttggcctcat ttttaaggtg tgcactttta ttcaactggt ctcaagtcag
     7861 tgtacaggta agccctggct gcctccaccc actcccaggg agaccaaaag ccttcataca
     7921 tctcaagttg ggggacaaaa aagggggaag ggggggcacg aaggctcatc attcaaaata
     7981 aaacaaaata aaaaagtatt aaggcgaaga ttaaaaaaat tttgcattac ataatttaca
     8041 cg
//
