LOCUS       sequence-355867-        8621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delUBAdelQrich.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of microRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Dec;29(23):6220-31. doi: 10.1128/MCB.01081-09. 
            Epub 2009 Sep 21.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01081-09"; journalName: "Mol Cell Biol"; date: 
            "2009-12"; volume: "29"; issue: "23"; pages: "6220-31"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G
FEATURES             Location/Qualifiers
     source          1..8621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2531..2596
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2624..2650
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5872..5913
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5923..5940
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5963..5980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6013..6147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6037..6056)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6091..6110
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6748..7336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6828..6847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7507..8298,8299..8367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8130..8149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8368..8472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8540..8558
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtacccaac atggacgcac aaacacgacg acgtgagcgt cgcgctgaga aacaagctca
     2581 atggaaagct gcaaacggtg gatctcctcc aagcttgatg gcttacccat acgatgttcc
     2641 agattacgct atgcgtgaag cccttttttc ccaagatggc tggggctgtc agcatgttaa
     2701 ccaggatact aattgggaag ttcccagttc gccagaacca gccaataagg atgcacccgg
     2761 tccaccaatg tggaagccaa gcattaacaa tggtactgat ctttgggagt ccaatttgag
     2821 aaacggaggt cagccggccg cacagcaagt tccaaagccg tcgtggggtc atacaccatc
     2881 ctctaactta ggtggaacat ggggtgagga cgacgatggc gccgatagta gtagtgtgtg
     2941 gactggagga gctgttagca acgcgggatc cggagctgca gtgggagtaa accaagccgg
     3001 agttaatgtc ggtccaggcg gtgttgtttc gtctggcgga cctcagtggg gacaaggtgt
     3061 cgttggcgtc ggacttggat caactggagg taacgggtca agcaatataa ctggatcgtc
     3121 tggagtcgca acaggtagta gcggaaactc cagcaacgct ggtaacggtt ggggagaccc
     3181 tcgtgaaata cgccctttgg gagttggtgg ctccatggat attcgaaatg ttgaacatcg
     3241 cggcggtaac ggttctggag caacttcgag cgatccacga gacattcgca tgatcgatcc
     3301 gcgtgaccct attcgaggag atccccgtgg aatatctggt cgtcttaatg ggacctctga
     3361 aatgtggggt catcatccac aaatgtccca taaccagttg caaggtatca acaaaatggt
     3421 tggtcaaagt gtagcaactg ccagcaccag tgtcggaaca tctggctcgg gcatcggtcc
     3481 tggaggtccc ggtcctagta cagtatcagg caatatccca acacagtggg ggcctgctca
     3541 accggtaagc gttggtgtaa gtggtcccaa agacatgtca aaacagataa gtggatggga
     3601 ggaaccatca ccaccgcctc agcgtcgcag tattcctaac tacgatgatg gtacatcgtt
     3661 gtggggtcag caaactcgtg ttcccgctgc aagcggtcac tggaaagaca tgactgattc
     3721 gataggtcgt agtagtcatc tcatgcgtgg ccaaagccaa acgggaggta taggaatagc
     3781 cggcgttgga aatagcaatg ttccagtggg agccaatcca agtaatccta taagcagtgt
     3841 agttggacct caagcccgga ttccatctgt gggcggcgta caacacaaac cagacggcgg
     3901 cgctatgtgg gtgcattccg gcaatgtagg tggcagaaat aatgttgctg ctgttactac
     3961 ttggggagat gacactcata gcgttaatgt cggcgctccc agcagtggca gtgtatccag
     4021 caacaattgg gttgatgaca agtccaactc aaccttggca caaaactctt ggagcgaccc
     4081 ggcccctgtt ggagttagtt ggggcaataa gcaaagcaaa ccgccaagca atagtgcttc
     4141 atcaggttgg agcactgctg cgggcgtggt ggatggggtt gatctaggat ctgagtggaa
     4201 cacgcacggg gggattattg gaaaatctca gcaacaacaa aaactagcgg gacttaacgt
     4261 gggatatcaa ggagcgtcca atcaacaatc ccgattaaat cagtggaagc ttccagtatt
     4321 agataaggag atcaactctg acagtacgga attttctcgt gccccaggtg caacgaaaca
     4381 aaatttgacg gccaacacaa gcaacataaa ctctttgggt cttcaaaacg atagtacatg
     4441 gtcaactgga cgcagtattg gtgacggttg gcctgatccc tcatctgata acgagaataa
     4501 agactggtct gttgctcagc caacttcagc agcaactgct tacactgatc tggtccaaga
     4561 gtttgagcca ggcaagccat ggaagggttc acagatcaaa agcatagaag atgatcccag
     4621 cattacacca ggaagcgttg ctagatctcc attgtctatt aattcgacgc caaaagatgc
     4681 tgacatattt gccaataccg gtaaaaattc accgactgat ttaccgccac taagtttatc
     4741 gtcgtctaca tggagtttta atccaaacca aaattatccg agtcacagtt ggtctgacaa
     4801 tagtcaacaa tgtaccgcca cttcggagct ttggacaagc ccgctaaata aatcatcgtc
     4861 tcgaggtccc ccgccaggat tgactgccaa ttcaaataaa tctgcaaata gtaatgcgtc
     4921 aacgccaaca actattaccg gaggtgcgaa tggatggtta cagcctcgaa gtggcggtgt
     4981 tcaaaccaca aacactaatt ggacaggtgg taacaccact tggggctcca gttggttgct
     5041 tttgaaaaat ctaacagcac agattgatgg tcctactttg cgtacactgt gtatgcagca
     5101 tgggcccctt gtcagctttc acccgtattt gaaccaagga attgccttat gtaaatatac
     5161 tactcgtgag gaggcgaaca aggcgcaaat ggcgttaaac aactgtgtcc tcgccaacac
     5221 cacaatattt gctgaatctc ccagcgagaa cgaggtgcaa agcattatgc agcacttacc
     5281 acaaactcct tcctctacaa gctctagtgg aactagtggt ggcaacgtcg gaggcgtcgg
     5341 cacttcagcc aataatgcaa acagtggttc tgcagcttgt ctgtccggaa acaatagcgg
     5401 caacggaaac ggcagcgcga gcggcgccgg cagcggcaac aatggcaaca gtagctgcaa
     5461 caacagtgcc gccggggggg gcagcagcag caacaacacg attaccactg tagcaaattc
     5521 gaatcttgtt ggttctagtg gctctgtctc aaattcctct ggcgttactg ctaactctag
     5581 tactgtttct gtagttagtt gtacagcgag tgggaattcc ataaatgggg caggtactgc
     5641 aaacagttct ggttcaaaga gtagtgcaaa caatttagct agcggccagt ctagcgcttc
     5701 taacttaact aatagcacca attcaacatg gcgacaaact agccaaaacc aagctcttca
     5761 aagtcaaagc aggccatcag gcagagaagc tgactttgat tatatatctc tcgtttattc
     5821 cattgttgat gattaagcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     5881 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     5941 tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca
     6001 gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     6061 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6121 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6181 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     6241 gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     6301 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     6361 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     6421 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     6481 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     6541 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     6601 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     6661 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa
     6721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7381 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7441 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7501 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7561 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7621 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7681 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7741 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7801 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7861 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7921 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7981 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8041 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8101 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     8161 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8221 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8281 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8341 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8401 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8461 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8521 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattaat
     8581 tctatattct aaaaacacaa atgatacttc taaaaaaaaa t
//
