LOCUS       sequence-35587-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pNI2
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      294..882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     783..802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
       61 aaaatatatc atcatgcctc ctctagaggt ctcccatggc ctgaccccgg accaagtggt
      121 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
      181 ggtgctgtgc caggaccgag accctcgagc cacccatgac caaaatccct taacgtgagt
      241 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      421 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      901 tttttacggt tcctggcctt ttgctggcct ttgct
//
