LOCUS       sequence-355904-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mNeongreen-DDX21 WT-mRuby3
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     286..303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    543..564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(579..584,585..602,603..609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    617..633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     622..644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     641..657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     641..657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     707..726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             918..1551
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1598..1723
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2220..2453
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2638..2682
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2975..3092
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3140..4318
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3371..4309
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4266..4286
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4357..4366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4618..4627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7468..8178
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    8218..8806
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8271..8291)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8689..8700)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8881..9114
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9233..9251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9319..9423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9424..9492,9493..10284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9642..9661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10455..10911,1..132)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
       61 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      301 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      361 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      421 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      481 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      541 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      601 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      661 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      721 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
      781 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
      841 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
      901 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
      961 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     1021 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     1081 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     1141 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     1201 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     1261 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     1321 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     1381 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1441 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1501 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1561 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1621 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1681 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1741 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1801 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1861 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1921 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1981 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2041 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     2101 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     2161 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     2221 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     2281 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     2341 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     2401 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     2461 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     2521 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2581 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2641 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2701 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2761 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2821 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2881 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2941 cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     3001 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     3061 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     3121 cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     3181 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     3241 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3301 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3361 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3421 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3481 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3541 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3601 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3661 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3721 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3781 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3841 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3901 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3961 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     4021 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     4081 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     4141 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     4201 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4261 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     4321 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgtc caagggcgaa
     4381 gaggacaaca tggccagcct gcctgccacc cacgagctgc acatcttcgg cagcatcaac
     4441 ggcgtggact tcgacatggt gggacagggc accggcaacc ccaacgacgg ctacgaggaa
     4501 ctgaacctga agtccacaaa gggcgacctg cagttcagcc cctggattct ggtgccccac
     4561 atcggctacg gcttccacca gtacctgccc taccccgacg gcatgagccc ttttcaggcc
     4621 gccatggtgg atggcagcgg ctaccaggtg caccggacca tgcagtttga ggacggcgcc
     4681 agcctgaccg tgaactaccg gtacacatac gagggcagcc acatcaaggg cgaggcccaa
     4741 gtgaagggca caggctttcc agccgacggc cccgtgatga ccaatagcct gacagccgcc
     4801 gactggtgca gaagcaagaa aacctacccc aatgacaaga ccatcatcag caccttcaag
     4861 tggtcctaca ccaccggcaa tggcaagcgg tacagaagca ccgcccggac cacctacacc
     4921 ttcgccaaac ccatggccgc caactacctg aagaaccagc ctatgtacgt gttccgcaag
     4981 accgagctga agcactccaa gacagaactg aacttcaaag agtggcagaa agccttcacc
     5041 gacgtgatgg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     5101 aattctgcag tcgacggtac cccgggaaaa ctccgtagtg acgctggttt ggaatcagac
     5161 accgcaatga aaaaagggga gacactgcga aagcaaaccg aggagaaaga gaaaaaagag
     5221 aagccaaaat ctgataagac tgaagagata gcagaagagg aagaaactgt tttccccaaa
     5281 gctaaacaag ttaaaaagaa agcagagcct tctgaagttg acatgaattc tcctaaatcc
     5341 aaaaaggcaa aaaagaaaga ggagccatct caaaatgaca tttctcctaa aaccaaaagt
     5401 ttgagaaaga aaaaggagcc cattgaaaag aaagtggttt cttctaaaac caaaaaagtg
     5461 acaaaaaatg aggagccttc tgaggaagaa atagatgctc ctaagcccaa gaagatgaag
     5521 aaagaaaagg aaatgaatgg agaaactaga gagaaaagcc ccaaactgaa gaatggattt
     5581 cctcatcctg aaccggactg taaccccagt gaagctgcca gtgaagaaag taacagtgag
     5641 atagagcagg aaatacctgt ggaacaaaaa gaaggcgctt tctctaattt tcccatatct
     5701 gaagaaacta ttaaacttct caaaggccga ggagtgacct tcctatttcc tatacaagca
     5761 aagacattcc atcatgttta cagcgggaag gacttaattg cacaggcacg gacaggaact
     5821 gggaagacat tctcctttgc catccctttg attgagaaac ttcatgggga actgcaagac
     5881 aggaagagag gccgtgcccc tcaggtactg gttcttgcac ctacaagaga gttggcaaat
     5941 caagtaagca aagacttcag tgacatcaca aaaaagctgt cagtggcttg tttttatggt
     6001 ggaactccct atggaggtca atttgaacgc atgaggaatg ggattgatat cctggttgga
     6061 acaccaggtc gtatcaaaga ccacatacag aatggcaaac tagatctcac caaacttaag
     6121 catgttgtcc tggatgaagt ggaccagatg ttggatatgg gatttgctga tcaagtggaa
     6181 gagattttaa gtgtggcata caagaaagat tctgaagaca atccccaaac attgcttttt
     6241 tctgcaactt gccctcattg ggtatttaat gttgccaaga aatacatgaa atctacatat
     6301 gaacaggtgg acctgattgg taaaaagact cagaaaacgg caataactgt ggagcatctg
     6361 gctattaagt gccactggac tcagagggca gcagttattg gggatgtcat ccgagtatat
     6421 agtggtcatc aaggacgcac tatcatcttt tgtgaaacca agaaagaagc ccaggagctg
     6481 tcccagaatt cagctataaa gcaggatgct cagtccttgc atggagacat tccacagaag
     6541 caaagggaaa tcaccctgaa aggttttaga aatggtagtt ttggagtttt ggtggcaacc
     6601 aatgttgctg cacgtgggtt agacatccct gaggttgatt tggttataca aagctctcca
     6661 ccaaaggatg tagagtccta cattcatcga tccgggcgga caggcagagc tggaaggacg
     6721 ggggtgtgca tctgctttta tcagcacaag gaagaatatc agttagtaca agtggagcaa
     6781 aaagcgggaa ttaagttcaa acgaataggt gttccttctg caacagaaat aataaaagct
     6841 tccagcaaag atgccatcag gcttttggat tccgtgcctc ccactgccat tagtcacttc
     6901 aaacaatcag ctgagaagct gatagaggag aagggagctg tggaagctct ggcagcagca
     6961 ctggcccata tttcaggtgc cacgtccgta gaccagcgct ccttgatcaa ctcaaatgtg
     7021 ggttttgtga ccatgatctt gcagtgctca attgaaatgc caaatattag ttatgcttgg
     7081 aaagaactta aagagcagct gggcgaggag attgattcca aagtgaaggg aatggttttt
     7141 ctcaaaggaa agctgggtgt ttgctttgat gtacctaccg catcagtaac agaaatacag
     7201 gagaaatggc atgattcacg acgctggcag ctctctgtgg ccacagagca accagaactg
     7261 gaaggaccac gggaaggata tggaggcttc aggggacagc gggaaggcag tcgaggcttc
     7321 aggggacagc gggacggaaa cagaagattc agaggacagc gggaaggcag tagaggcccg
     7381 agaggacagc gatcaggagg tggcaacaaa agtaacagat cccaaaacaa aggccagaag
     7441 cggagtttca gtaaagcatt tggtcaaatg gtgtctaagg gcgaagagct gatcaaggaa
     7501 aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca
     7561 ggtgaaggag aaggcagacc gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag
     7621 ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt
     7681 acctttatca agtacccggc cgacatccct gatttcttta aacagtcctt tcctgagggt
     7741 tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt cacgcaggac
     7801 accagccttg aggatggcga gctcgtctac aacgtcaagg tcagaggggt aaactttccc
     7861 tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg
     7921 tatccagcag atggtggtct gagaggatac actgacatcg cactgaaagt tgatggtggt
     7981 ggccatctgc actgcaactt cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc
     8041 aagatgcccg gtgtccatgc cgttgatcac cgcctggaaa ggatcgagga gagtgacaat
     8101 gaaacctacg tagtgcaaag agaagtggca gttgccaaat acagcaacct tggtggtggc
     8161 atggacgagc tgtacaaggg atccaccgga tctagataac gcccatcgat agatcctaat
     8221 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8281 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8341 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8401 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8461 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8521 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8581 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8641 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8701 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8761 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     8821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8881 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     9121 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9181 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     9241 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     9301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9661 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt c
//
