LOCUS       sequence-355905-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mNeongreen-DDX21 DAT-mRuby3
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     315..334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     568..585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    825..846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(861..866,867..884,885..891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    899..915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     904..926
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     923..939
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     923..939
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     989..1008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1200..1833
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1880..2005
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2502..2735
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2920..2964
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3257..3374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3422..4600
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3653..4591
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4548..4568
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4639..4648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4900..4909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7750..8460
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    8500..9088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8553..8573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8971..8982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9163..9396
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9515..9533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9601..9705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9706..9774,9775..10566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9924..9943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10737..10911,1..414)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
       61 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
      661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
      781 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      841 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      901 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
      961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1021 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     1081 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     1141 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     1201 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     1261 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     1321 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     1381 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     1441 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     1501 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     1561 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     1621 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     1681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     1741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     1801 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     1861 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     1921 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1981 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     2041 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     2101 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     2161 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     2221 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     2281 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     2341 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     2401 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     2461 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     2521 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     2581 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     2641 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     2701 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     2761 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     2821 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     2881 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     2941 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     3001 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     3061 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     3121 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     3181 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     3241 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     3301 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     3361 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg
     3421 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     3481 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     3541 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     3601 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     3661 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     3721 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     3781 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     3841 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     3901 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     3961 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     4021 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     4081 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     4141 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     4201 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     4261 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     4321 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     4381 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     4441 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     4501 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     4561 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggtttagtga accgtcagat
     4621 ccgctagcgc taccggtcgc caccatggtg tccaagggcg aagaggacaa catggccagc
     4681 ctgcctgcca cccacgagct gcacatcttc ggcagcatca acggcgtgga cttcgacatg
     4741 gtgggacagg gcaccggcaa ccccaacgac ggctacgagg aactgaacct gaagtccaca
     4801 aagggcgacc tgcagttcag cccctggatt ctggtgcccc acatcggcta cggcttccac
     4861 cagtacctgc cctaccccga cggcatgagc ccttttcagg ccgccatggt ggatggcagc
     4921 ggctaccagg tgcaccggac catgcagttt gaggacggcg ccagcctgac cgtgaactac
     4981 cggtacacat acgagggcag ccacatcaag ggcgaggccc aagtgaaggg cacaggcttt
     5041 ccagccgacg gccccgtgat gaccaatagc ctgacagccg ccgactggtg cagaagcaag
     5101 aaaacctacc ccaatgacaa gaccatcatc agcaccttca agtggtccta caccaccggc
     5161 aatggcaagc ggtacagaag caccgcccgg accacctaca ccttcgccaa acccatggcc
     5221 gccaactacc tgaagaacca gcctatgtac gtgttccgca agaccgagct gaagcactcc
     5281 aagacagaac tgaacttcaa agagtggcag aaagccttca ccgacgtgat gggcatggac
     5341 gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
     5401 accccgggaa aactccgtag tgacgctggt ttggaatcag acaccgcaat gaaaaaaggg
     5461 gagacactgc gaaagcaaac cgaggagaaa gagaaaaaag agaagccaaa atctgataag
     5521 actgaagaga tagcagaaga ggaagaaact gttttcccca aagctaaaca agttaaaaag
     5581 aaagcagagc cttctgaagt tgacatgaat tctcctaaat ccaaaaaggc aaaaaagaaa
     5641 gaggagccat ctcaaaatga catttctcct aaaaccaaaa gtttgagaaa gaaaaaggag
     5701 cccattgaaa agaaagtggt ttcttctaaa accaaaaaag tgacaaaaaa tgaggagcct
     5761 tctgaggaag aaatagatgc tcctaagccc aagaagatga agaaagaaaa ggaaatgaat
     5821 ggagaaacta gagagaaaag ccccaaactg aagaatggat ttcctcatcc tgaaccggac
     5881 tgtaacccca gtgaagctgc cagtgaagaa agtaacagtg agatagagca ggaaatacct
     5941 gtggaacaaa aagaaggcgc tttctctaat tttcccatat ctgaagaaac tattaaactt
     6001 ctcaaaggcc gaggagtgac cttcctattt cctatacaag caaagacatt ccatcatgtt
     6061 tacagcggga aggacttaat tgcacaggca cggacaggaa ctgacgctac attctccttt
     6121 gccatccctt tgattgagaa acttcatggg gaactgcaag acaggaagag aggccgtgcc
     6181 cctcaggtac tggttcttgc acctacaaga gagttggcaa atcaagtaag caaagacttc
     6241 agtgacatca caaaaaagct gtcagtggct tgtttttatg gtggaactcc ctatggaggt
     6301 caatttgaac gcatgaggaa tgggattgat atcctggttg gaacaccagg tcgtatcaaa
     6361 gaccacatac agaatggcaa actagatctc accaaactta agcatgttgt cctggatgaa
     6421 gtggaccaga tgttggatat gggatttgct gatcaagtgg aagagatttt aagtgtggca
     6481 tacaagaaag attctgaaga caatccccaa acattgcttt tttctgcaac ttgccctcat
     6541 tgggtattta atgttgccaa gaaatacatg aaatctacat atgaacaggt ggacctgatt
     6601 ggtaaaaaga ctcagaaaac ggcaataact gtggagcatc tggctattaa gtgccactgg
     6661 actcagaggg cagcagttat tggggatgtc atccgagtat atagtggtca tcaaggacgc
     6721 actatcatct tttgtgaaac caagaaagaa gcccaggagc tgtcccagaa ttcagctata
     6781 aagcaggatg ctcagtcctt gcatggagac attccacaga agcaaaggga aatcaccctg
     6841 aaaggtttta gaaatggtag ttttggagtt ttggtggcaa ccaatgttgc tgcacgtggg
     6901 ttagacatcc ctgaggttga tttggttata caaagctctc caccaaagga tgtagagtcc
     6961 tacattcatc gatccgggcg gacaggcaga gctggaagga cgggggtgtg catctgcttt
     7021 tatcagcaca aggaagaata tcagttagta caagtggagc aaaaagcggg aattaagttc
     7081 aaacgaatag gtgttccttc tgcaacagaa ataataaaag cttccagcaa agatgccatc
     7141 aggcttttgg attccgtgcc tcccactgcc attagtcact tcaaacaatc agctgagaag
     7201 ctgatagagg agaagggagc tgtggaagct ctggcagcag cactggccca tatttcaggt
     7261 gccacgtccg tagaccagcg ctccttgatc aactcaaatg tgggttttgt gaccatgatc
     7321 ttgcagtgct caattgaaat gccaaatatt agttatgctt ggaaagaact taaagagcag
     7381 ctgggcgagg agattgattc caaagtgaag ggaatggttt ttctcaaagg aaagctgggt
     7441 gtttgctttg atgtacctac cgcatcagta acagaaatac aggagaaatg gcatgattca
     7501 cgacgctggc agctctctgt ggccacagag caaccagaac tggaaggacc acgggaagga
     7561 tatggaggct tcaggggaca gcgggaaggc agtcgaggct tcaggggaca gcgggacgga
     7621 aacagaagat tcagaggaca gcgggaaggc agtagaggcc cgagaggaca gcgatcagga
     7681 ggtggcaaca aaagtaacag atcccaaaac aaaggccaga agcggagttt cagtaaagca
     7741 tttggtcaaa tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg
     7801 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaga
     7861 ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
     7921 gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacctttat caagtacccg
     7981 gccgacatcc ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt
     8041 acgagatacg aagatggtgg agtcgtcacc gtcacgcagg acaccagcct tgaggatggc
     8101 gagctcgtct acaacgtcaa ggtcagaggg gtaaactttc cctccaatgg tcccgtgatg
     8161 cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt
     8221 ctgagaggat acactgacat cgcactgaaa gttgatggtg gtggccatct gcactgcaac
     8281 ttcgtgacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat
     8341 gccgttgatc accgcctgga aaggatcgag gagagtgaca atgaaaccta cgtagtgcaa
     8401 agagaagtgg cagttgccaa atacagcaac cttggtggtg gcatggacga gctgtacaag
     8461 ggatccaccg gatctagata acgcccatcg atagatccta atcaacctct ggattacaaa
     8521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9061 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     9121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9181 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9421 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     9481 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     9541 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9841 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9901 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     9961 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10021 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10081 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10141 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10201 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10261 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10321 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10381 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10441 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10501 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10561 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10861 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca c
//
