LOCUS       sequence-355906-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mNeongreen-DDX21 HGV-mRuby3
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    643..664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(679..684,685..702,703..709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    717..733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     722..744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     741..757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     741..757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     807..826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1018..1651
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1698..1823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2320..2553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2738..2782
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3075..3192
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3240..4418
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3471..4409
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4366..4386
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4457..4466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4718..4727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7568..8278
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    8318..8906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8371..8391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8789..8800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8981..9214
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9333..9351)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9419..9523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9524..9592,9593..10384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9742..9761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10555..10911,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
      481 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
      601 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      661 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      721 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
      781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      841 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
      901 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
      961 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     1021 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     1081 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     1141 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     1201 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     1261 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     1321 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     1381 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     1441 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     1501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1621 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1681 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1741 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1801 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1861 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1921 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1981 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     2041 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     2101 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     2161 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2221 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2281 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2341 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2401 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2461 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2521 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2581 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     2641 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2701 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2761 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2821 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2881 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2941 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     3001 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
     3061 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     3121 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     3181 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac tagtcgtgag
     3241 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     3301 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3361 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3421 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3481 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3541 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3601 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     3661 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3721 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3781 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3841 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3901 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3961 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     4021 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     4081 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     4141 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     4201 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     4261 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     4321 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4381 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg tttagtgaac cgtcagatcc
     4441 gctagcgcta ccggtcgcca ccatggtgtc caagggcgaa gaggacaaca tggccagcct
     4501 gcctgccacc cacgagctgc acatcttcgg cagcatcaac ggcgtggact tcgacatggt
     4561 gggacagggc accggcaacc ccaacgacgg ctacgaggaa ctgaacctga agtccacaaa
     4621 gggcgacctg cagttcagcc cctggattct ggtgccccac atcggctacg gcttccacca
     4681 gtacctgccc taccccgacg gcatgagccc ttttcaggcc gccatggtgg atggcagcgg
     4741 ctaccaggtg caccggacca tgcagtttga ggacggcgcc agcctgaccg tgaactaccg
     4801 gtacacatac gagggcagcc acatcaaggg cgaggcccaa gtgaagggca caggctttcc
     4861 agccgacggc cccgtgatga ccaatagcct gacagccgcc gactggtgca gaagcaagaa
     4921 aacctacccc aatgacaaga ccatcatcag caccttcaag tggtcctaca ccaccggcaa
     4981 tggcaagcgg tacagaagca ccgcccggac cacctacacc ttcgccaaac ccatggccgc
     5041 caactacctg aagaaccagc ctatgtacgt gttccgcaag accgagctga agcactccaa
     5101 gacagaactg aacttcaaag agtggcagaa agccttcacc gacgtgatgg gcatggacga
     5161 gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
     5221 cccgggaaaa ctccgtagtg acgctggttt ggaatcagac accgcaatga aaaaagggga
     5281 gacactgcga aagcaaaccg aggagaaaga gaaaaaagag aagccaaaat ctgataagac
     5341 tgaagagata gcagaagagg aagaaactgt tttccccaaa gctaaacaag ttaaaaagaa
     5401 agcagagcct tctgaagttg acatgaattc tcctaaatcc aaaaaggcaa aaaagaaaga
     5461 ggagccatct caaaatgaca tttctcctaa aaccaaaagt ttgagaaaga aaaaggagcc
     5521 cattgaaaag aaagtggttt cttctaaaac caaaaaagtg acaaaaaatg aggagccttc
     5581 tgaggaagaa atagatgctc ctaagcccaa gaagatgaag aaagaaaagg aaatgaatgg
     5641 agaaactaga gagaaaagcc ccaaactgaa gaatggattt cctcatcctg aaccggactg
     5701 taaccccagt gaagctgcca gtgaagaaag taacagtgag atagagcagg aaatacctgt
     5761 ggaacaaaaa gaaggcgctt tctctaattt tcccatatct gaagaaacta ttaaacttct
     5821 caaaggccga ggagtgacct tcctatttcc tatacaagca aagacattcc atcatgttta
     5881 cagcgggaag gacttaattg cacaggcacg gacaggaact gggaagacat tctcctttgc
     5941 catccctttg attgagaaac ttcatgggga actgcaagac aggaagagag gccgtgcccc
     6001 tcaggtactg gttcttgcac ctacaagaga gttggcaaat caagtaagca aagacttcag
     6061 tgacatcaca aaaaagctgt cagtggcttg tttttatggt ggaactccct atggaggtca
     6121 atttgaacgc atgaggaatg ggattgatat cctggttgga acaccaggtc gtatcaaaga
     6181 ccacatacag aatggcaaac tagatctcac caaacttaag catgttgtcc tgcatggagt
     6241 ggaccagatg ttggatatgg gatttgctga tcaagtggaa gagattttaa gtgtggcata
     6301 caagaaagat tctgaagaca atccccaaac attgcttttt tctgcaactt gccctcattg
     6361 ggtatttaat gttgccaaga aatacatgaa atctacatat gaacaggtgg acctgattgg
     6421 taaaaagact cagaaaacgg caataactgt ggagcatctg gctattaagt gccactggac
     6481 tcagagggca gcagttattg gggatgtcat ccgagtatat agtggtcatc aaggacgcac
     6541 tatcatcttt tgtgaaacca agaaagaagc ccaggagctg tcccagaatt cagctataaa
     6601 gcaggatgct cagtccttgc atggagacat tccacagaag caaagggaaa tcaccctgaa
     6661 aggttttaga aatggtagtt ttggagtttt ggtggcaacc aatgttgctg cacgtgggtt
     6721 agacatccct gaggttgatt tggttataca aagctctcca ccaaaggatg tagagtccta
     6781 cattcatcga tccgggcgga caggcagagc tggaaggacg ggggtgtgca tctgctttta
     6841 tcagcacaag gaagaatatc agttagtaca agtggagcaa aaagcgggaa ttaagttcaa
     6901 acgaataggt gttccttctg caacagaaat aataaaagct tccagcaaag atgccatcag
     6961 gcttttggat tccgtgcctc ccactgccat tagtcacttc aaacaatcag ctgagaagct
     7021 gatagaggag aagggagctg tggaagctct ggcagcagca ctggcccata tttcaggtgc
     7081 cacgtccgta gaccagcgct ccttgatcaa ctcaaatgtg ggttttgtga ccatgatctt
     7141 gcagtgctca attgaaatgc caaatattag ttatgcttgg aaagaactta aagagcagct
     7201 gggcgaggag attgattcca aagtgaaggg aatggttttt ctcaaaggaa agctgggtgt
     7261 ttgctttgat gtacctaccg catcagtaac agaaatacag gagaaatggc atgattcacg
     7321 acgctggcag ctctctgtgg ccacagagca accagaactg gaaggaccac gggaaggata
     7381 tggaggcttc aggggacagc gggaaggcag tcgaggcttc aggggacagc gggacggaaa
     7441 cagaagattc agaggacagc gggaaggcag tagaggcccg agaggacagc gatcaggagg
     7501 tggcaacaaa agtaacagat cccaaaacaa aggccagaag cggagtttca gtaaagcatt
     7561 tggtcaaatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt
     7621 catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcagacc
     7681 gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc
     7741 ctttgacatt cttgccacgt cgttcatgta tggcagccgt acctttatca agtacccggc
     7801 cgacatccct gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac
     7861 gagatacgaa gatggtggag tcgtcaccgt cacgcaggac accagccttg aggatggcga
     7921 gctcgtctac aacgtcaagg tcagaggggt aaactttccc tccaatggtc ccgtgatgca
     7981 gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct
     8041 gagaggatac actgacatcg cactgaaagt tgatggtggt ggccatctgc actgcaactt
     8101 cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc
     8161 cgttgatcac cgcctggaaa ggatcgagga gagtgacaat gaaacctacg tagtgcaaag
     8221 agaagtggca gttgccaaat acagcaacct tggtggtggc atggacgagc tgtacaaggg
     8281 atccaccgga tctagataac gcccatcgat agatcctaat caacctctgg attacaaaat
     8341 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8401 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8461 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8521 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8581 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8641 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8701 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8761 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8821 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8881 gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca
     8941 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9001 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9061 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9181 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9241 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag
     9301 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     9361 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     9781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10261 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10381 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa c
//
