LOCUS       sequence-355907-       10200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mNeongreen-DDX21 WT
FEATURES             Location/Qualifiers
     source          1..10200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    642..663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(678..683,684..701,702..708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    716..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     721..743
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     740..756
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     740..756
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     806..825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1017..1650
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1697..1822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2319..2552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2737..2781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3074..3191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3239..4417
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3470..4408
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4365..4385
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4456..4465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4717..4726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7606..8194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7659..7679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8077..8088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8269..8502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8621..8639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8707..8811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8812..8880,8881..9672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9030..9049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9843..10200,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
      481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
      601 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      661 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      721 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
      781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      841 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
      901 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
      961 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     1021 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     1081 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     1141 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     1201 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     1261 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     1321 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     1381 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     1441 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     1501 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1561 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1621 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1681 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1741 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1801 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1861 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1921 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1981 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2041 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2101 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2161 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     2221 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     2281 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     2341 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     2401 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     2461 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     2521 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     2581 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2641 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2701 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2761 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2821 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2881 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2941 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     3001 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     3061 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     3121 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3181 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtgagg
     3241 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3301 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3361 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     3421 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3481 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3541 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3601 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     3661 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3721 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3781 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     3841 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3901 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3961 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     4021 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     4081 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     4141 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     4201 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     4261 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4321 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4381 ggttcaaagt ttttttcttc catttcaggt gtcgtgaggt ttagtgaacc gtcagatccg
     4441 ctagcgctac cggtcgccac catggtgtcc aagggcgaag aggacaacat ggccagcctg
     4501 cctgccaccc acgagctgca catcttcggc agcatcaacg gcgtggactt cgacatggtg
     4561 ggacagggca ccggcaaccc caacgacggc tacgaggaac tgaacctgaa gtccacaaag
     4621 ggcgacctgc agttcagccc ctggattctg gtgccccaca tcggctacgg cttccaccag
     4681 tacctgccct accccgacgg catgagccct tttcaggccg ccatggtgga tggcagcggc
     4741 taccaggtgc accggaccat gcagtttgag gacggcgcca gcctgaccgt gaactaccgg
     4801 tacacatacg agggcagcca catcaagggc gaggcccaag tgaagggcac aggctttcca
     4861 gccgacggcc ccgtgatgac caatagcctg acagccgccg actggtgcag aagcaagaaa
     4921 acctacccca atgacaagac catcatcagc accttcaagt ggtcctacac caccggcaat
     4981 ggcaagcggt acagaagcac cgcccggacc acctacacct tcgccaaacc catggccgcc
     5041 aactacctga agaaccagcc tatgtacgtg ttccgcaaga ccgagctgaa gcactccaag
     5101 acagaactga acttcaaaga gtggcagaaa gccttcaccg acgtgatggg catggacgag
     5161 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     5221 ccgggaaaac tccgtagtga cgctggtttg gaatcagaca ccgcaatgaa aaaaggggag
     5281 acactgcgaa agcaaaccga ggagaaagag aaaaaagaga agccaaaatc tgataagact
     5341 gaagagatag cagaagagga agaaactgtt ttccccaaag ctaaacaagt taaaaagaaa
     5401 gcagagcctt ctgaagttga catgaattct cctaaatcca aaaaggcaaa aaagaaagag
     5461 gagccatctc aaaatgacat ttctcctaaa accaaaagtt tgagaaagaa aaaggagccc
     5521 attgaaaaga aagtggtttc ttctaaaacc aaaaaagtga caaaaaatga ggagccttct
     5581 gaggaagaaa tagatgctcc taagcccaag aagatgaaga aagaaaagga aatgaatgga
     5641 gaaactagag agaaaagccc caaactgaag aatggatttc ctcatcctga accggactgt
     5701 aaccccagtg aagctgccag tgaagaaagt aacagtgaga tagagcagga aatacctgtg
     5761 gaacaaaaag aaggcgcttt ctctaatttt cccatatctg aagaaactat taaacttctc
     5821 aaaggccgag gagtgacctt cctatttcct atacaagcaa agacattcca tcatgtttac
     5881 agcgggaagg acttaattgc acaggcacgg acaggaactg ggaagacatt ctcctttgcc
     5941 atccctttga ttgagaaact tcatggggaa ctgcaagaca ggaagagagg ccgtgcccct
     6001 caggtactgg ttcttgcacc tacaagagag ttggcaaatc aagtaagcaa agacttcagt
     6061 gacatcacaa aaaagctgtc agtggcttgt ttttatggtg gaactcccta tggaggtcaa
     6121 tttgaacgca tgaggaatgg gattgatatc ctggttggaa caccaggtcg tatcaaagac
     6181 cacatacaga atggcaaact agatctcacc aaacttaagc atgttgtcct ggatgaagtg
     6241 gaccagatgt tggatatggg atttgctgat caagtggaag agattttaag tgtggcatac
     6301 aagaaagatt ctgaagacaa tccccaaaca ttgctttttt ctgcaacttg ccctcattgg
     6361 gtatttaatg ttgccaagaa atacatgaaa tctacatatg aacaggtgga cctgattggt
     6421 aaaaagactc agaaaacggc aataactgtg gagcatctgg ctattaagtg ccactggact
     6481 cagagggcag cagttattgg ggatgtcatc cgagtatata gtggtcatca aggacgcact
     6541 atcatctttt gtgaaaccaa gaaagaagcc caggagctgt cccagaattc agctataaag
     6601 caggatgctc agtccttgca tggagacatt ccacagaagc aaagggaaat caccctgaaa
     6661 ggttttagaa atggtagttt tggagttttg gtggcaacca atgttgctgc acgtgggtta
     6721 gacatccctg aggttgattt ggttatacaa agctctccac caaaggatgt agagtcctac
     6781 attcatcgat ccgggcggac aggcagagct ggaaggacgg gggtgtgcat ctgcttttat
     6841 cagcacaagg aagaatatca gttagtacaa gtggagcaaa aagcgggaat taagttcaaa
     6901 cgaataggtg ttccttctgc aacagaaata ataaaagctt ccagcaaaga tgccatcagg
     6961 cttttggatt ccgtgcctcc cactgccatt agtcacttca aacaatcagc tgagaagctg
     7021 atagaggaga agggagctgt ggaagctctg gcagcagcac tggcccatat ttcaggtgcc
     7081 acgtccgtag accagcgctc cttgatcaac tcaaatgtgg gttttgtgac catgatcttg
     7141 cagtgctcaa ttgaaatgcc aaatattagt tatgcttgga aagaacttaa agagcagctg
     7201 ggcgaggaga ttgattccaa agtgaaggga atggtttttc tcaaaggaaa gctgggtgtt
     7261 tgctttgatg tacctaccgc atcagtaaca gaaatacagg agaaatggca tgattcacga
     7321 cgctggcagc tctctgtggc cacagagcaa ccagaactgg aaggaccacg ggaaggatat
     7381 ggaggcttca ggggacagcg ggaaggcagt cgaggcttca ggggacagcg ggacggaaac
     7441 agaagattca gaggacagcg ggaaggcagt agaggcccga gaggacagcg atcaggaggt
     7501 ggcaacaaaa gtaacagatc ccaaaacaaa ggccagaagc ggagtttcag taaagcattt
     7561 ggtcaaggat ccaccggatc tagataacgc ccatcgatag atcctaatca acctctggat
     7621 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7681 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7741 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7801 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7861 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7921 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7981 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8041 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8101 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8161 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     8221 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8281 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8341 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8401 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8461 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8521 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     8581 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     8641 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8701 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8761 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8821 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8881 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8941 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9001 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     9061 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9121 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9181 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9241 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9301 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9361 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9421 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9481 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9541 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9601 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9661 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9721 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9961 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
//
