LOCUS       sequence-355910-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mRuby3-DDX21 WT
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    226..247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(262..267,268..285,286..292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     305..327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     324..340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     324..340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     390..409
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             601..1234
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1281..1406
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1903..2136
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2321..2365
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2658..2775
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2823..4001
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3054..3992
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3949..3969
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4040..4049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4046..4756
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    7192..7780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7245..7265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7663..7674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7855..8088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8207..8225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8293..8397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8398..8466,8467..9258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8616..8635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9429..10017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9918..9937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10171..10188
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
       61 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      301 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
      361 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      421 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
      481 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
      541 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
      601 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
      661 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      721 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      781 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      841 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      901 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      961 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     1021 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     1081 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1141 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1201 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1261 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1321 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1381 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1441 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1501 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1561 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1621 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1681 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1741 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1801 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1861 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1921 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1981 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     2041 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     2101 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2161 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2221 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2281 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2341 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2401 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2461 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2521 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2581 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2641 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2701 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2761 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2821 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2881 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2941 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     3001 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     3061 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3121 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     3181 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     3241 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     3301 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     3361 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     3421 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     3481 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     3541 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3601 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3661 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3721 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3781 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3841 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3901 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3961 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggtttagtg aaccgtcaga
     4021 tccgctagcg ctaccggtcg ccaccatggt gtctaagggc gaagagctga tcaaggaaaa
     4081 tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg
     4141 tgaaggagaa ggcagaccgt acgagggagt gcaaaccatg aggatcaaag tcatcgaggg
     4201 aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac
     4261 ctttatcaag tacccggccg acatccctga tttctttaaa cagtcctttc ctgagggttt
     4321 tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca cgcaggacac
     4381 cagccttgag gatggcgagc tcgtctacaa cgtcaaggtc agaggggtaa actttccctc
     4441 caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta
     4501 tccagcagat ggtggtctga gaggatacac tgacatcgca ctgaaagttg atggtggtgg
     4561 ccatctgcac tgcaacttcg tgacaactta caggtcaaaa aagaccgtcg ggaacatcaa
     4621 gatgcccggt gtccatgccg ttgatcaccg cctggaaagg atcgaggaga gtgacaatga
     4681 aacctacgta gtgcaaagag aagtggcagt tgccaaatac agcaaccttg gtggtggcat
     4741 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     4801 cggtaccccg ggaaaactcc gtagtgacgc tggtttggaa tcagacaccg caatgaaaaa
     4861 aggggagaca ctgcgaaagc aaaccgagga gaaagagaaa aaagagaagc caaaatctga
     4921 taagactgaa gagatagcag aagaggaaga aactgttttc cccaaagcta aacaagttaa
     4981 aaagaaagca gagccttctg aagttgacat gaattctcct aaatccaaaa aggcaaaaaa
     5041 gaaagaggag ccatctcaaa atgacatttc tcctaaaacc aaaagtttga gaaagaaaaa
     5101 ggagcccatt gaaaagaaag tggtttcttc taaaaccaaa aaagtgacaa aaaatgagga
     5161 gccttctgag gaagaaatag atgctcctaa gcccaagaag atgaagaaag aaaaggaaat
     5221 gaatggagaa actagagaga aaagccccaa actgaagaat ggatttcctc atcctgaacc
     5281 ggactgtaac cccagtgaag ctgccagtga agaaagtaac agtgagatag agcaggaaat
     5341 acctgtggaa caaaaagaag gcgctttctc taattttccc atatctgaag aaactattaa
     5401 acttctcaaa ggccgaggag tgaccttcct atttcctata caagcaaaga cattccatca
     5461 tgtttacagc gggaaggact taattgcaca ggcacggaca ggaactggga agacattctc
     5521 ctttgccatc cctttgattg agaaacttca tggggaactg caagacagga agagaggccg
     5581 tgcccctcag gtactggttc ttgcacctac aagagagttg gcaaatcaag taagcaaaga
     5641 cttcagtgac atcacaaaaa agctgtcagt ggcttgtttt tatggtggaa ctccctatgg
     5701 aggtcaattt gaacgcatga ggaatgggat tgatatcctg gttggaacac caggtcgtat
     5761 caaagaccac atacagaatg gcaaactaga tctcaccaaa cttaagcatg ttgtcctgga
     5821 tgaagtggac cagatgttgg atatgggatt tgctgatcaa gtggaagaga ttttaagtgt
     5881 ggcatacaag aaagattctg aagacaatcc ccaaacattg cttttttctg caacttgccc
     5941 tcattgggta tttaatgttg ccaagaaata catgaaatct acatatgaac aggtggacct
     6001 gattggtaaa aagactcaga aaacggcaat aactgtggag catctggcta ttaagtgcca
     6061 ctggactcag agggcagcag ttattgggga tgtcatccga gtatatagtg gtcatcaagg
     6121 acgcactatc atcttttgtg aaaccaagaa agaagcccag gagctgtccc agaattcagc
     6181 tataaagcag gatgctcagt ccttgcatgg agacattcca cagaagcaaa gggaaatcac
     6241 cctgaaaggt tttagaaatg gtagttttgg agttttggtg gcaaccaatg ttgctgcacg
     6301 tgggttagac atccctgagg ttgatttggt tatacaaagc tctccaccaa aggatgtaga
     6361 gtcctacatt catcgatccg ggcggacagg cagagctgga aggacggggg tgtgcatctg
     6421 cttttatcag cacaaggaag aatatcagtt agtacaagtg gagcaaaaag cgggaattaa
     6481 gttcaaacga ataggtgttc cttctgcaac agaaataata aaagcttcca gcaaagatgc
     6541 catcaggctt ttggattccg tgcctcccac tgccattagt cacttcaaac aatcagctga
     6601 gaagctgata gaggagaagg gagctgtgga agctctggca gcagcactgg cccatatttc
     6661 aggtgccacg tccgtagacc agcgctcctt gatcaactca aatgtgggtt ttgtgaccat
     6721 gatcttgcag tgctcaattg aaatgccaaa tattagttat gcttggaaag aacttaaaga
     6781 gcagctgggc gaggagattg attccaaagt gaagggaatg gtttttctca aaggaaagct
     6841 gggtgtttgc tttgatgtac ctaccgcatc agtaacagaa atacaggaga aatggcatga
     6901 ttcacgacgc tggcagctct ctgtggccac agagcaacca gaactggaag gaccacggga
     6961 aggatatgga ggcttcaggg gacagcggga aggcagtcga ggcttcaggg gacagcggga
     7021 cggaaacaga agattcagag gacagcggga aggcagtaga ggcccgagag gacagcgatc
     7081 aggaggtggc aacaaaagta acagatccca aaacaaaggc cagaagcgga gtttcagtaa
     7141 agcatttgtc aaggatccac cggatctaga taacgcccat cgatagatcc taatcaacct
     7201 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7261 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7321 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7381 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7441 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7501 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7561 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7621 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7681 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7741 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     7801 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7861 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7921 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7981 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8041 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     8101 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     8161 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     8221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8641 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10201 cg
//
