LOCUS       sequence-355912-       10206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-mRuby3-DDX21 HGV
FEATURES             Location/Qualifiers
     source          1..10206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    399..632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             817..861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1154..1271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1319..2497
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1550..2488
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2445..2465
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2536..2545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2542..3252
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    5692..6280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5745..5765)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6163..6174)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6355..6588
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6707..6725)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6793..6897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6898..6966,6967..7758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7116..7135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7929..8517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8418..8437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8671..8688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8928..8949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8964..8969,8970..8987,8988..8994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9002..9018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9007..9029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9026..9042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9026..9042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9092..9111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9303..9936
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9983..10108
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
       61 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
      121 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
      181 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
      241 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
      301 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
      361 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
      421 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
      481 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
      541 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
      601 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
      661 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
      721 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
      781 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
      841 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
      901 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
      961 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1021 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1081 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     1141 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1201 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     1261 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtgagg
     1321 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1381 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1441 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     1501 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     1561 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     1621 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     1681 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     1741 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     1801 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     1861 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     1921 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1981 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     2041 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     2101 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     2161 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     2221 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     2281 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     2341 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     2401 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     2461 ggttcaaagt ttttttcttc catttcaggt gtcgtgaggt ttagtgaacc gtcagatccg
     2521 ctagcgctac cggtcgccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg
     2581 cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa
     2641 ggagaaggca gaccgtacga gggagtgcaa accatgagga tcaaagtcat cgagggagga
     2701 cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtaccttt
     2761 atcaagtacc cggccgacat ccctgatttc tttaaacagt cctttcctga gggttttact
     2821 tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcacgca ggacaccagc
     2881 cttgaggatg gcgagctcgt ctacaacgtc aaggtcagag gggtaaactt tccctccaat
     2941 ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca
     3001 gcagatggtg gtctgagagg atacactgac atcgcactga aagttgatgg tggtggccat
     3061 ctgcactgca acttcgtgac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg
     3121 cccggtgtcc atgccgttga tcaccgcctg gaaaggatcg aggagagtga caatgaaacc
     3181 tacgtagtgc aaagagaagt ggcagttgcc aaatacagca accttggtgg tggcatggac
     3241 gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
     3301 accccgggaa aactccgtag tgacgctggt ttggaatcag acaccgcaat gaaaaaaggg
     3361 gagacactgc gaaagcaaac cgaggagaaa gagaaaaaag agaagccaaa atctgataag
     3421 actgaagaga tagcagaaga ggaagaaact gttttcccca aagctaaaca agttaaaaag
     3481 aaagcagagc cttctgaagt tgacatgaat tctcctaaat ccaaaaaggc aaaaaagaaa
     3541 gaggagccat ctcaaaatga catttctcct aaaaccaaaa gtttgagaaa gaaaaaggag
     3601 cccattgaaa agaaagtggt ttcttctaaa accaaaaaag tgacaaaaaa tgaggagcct
     3661 tctgaggaag aaatagatgc tcctaagccc aagaagatga agaaagaaaa ggaaatgaat
     3721 ggagaaacta gagagaaaag ccccaaactg aagaatggat ttcctcatcc tgaaccggac
     3781 tgtaacccca gtgaagctgc cagtgaagaa agtaacagtg agatagagca ggaaatacct
     3841 gtggaacaaa aagaaggcgc tttctctaat tttcccatat ctgaagaaac tattaaactt
     3901 ctcaaaggcc gaggagtgac cttcctattt cctatacaag caaagacatt ccatcatgtt
     3961 tacagcggga aggacttaat tgcacaggca cggacaggaa ctgggaagac attctccttt
     4021 gccatccctt tgattgagaa acttcatggg gaactgcaag acaggaagag aggccgtgcc
     4081 cctcaggtac tggttcttgc acctacaaga gagttggcaa atcaagtaag caaagacttc
     4141 agtgacatca caaaaaagct gtcagtggct tgtttttatg gtggaactcc ctatggaggt
     4201 caatttgaac gcatgaggaa tgggattgat atcctggttg gaacaccagg tcgtatcaaa
     4261 gaccacatac agaatggcaa actagatctc accaaactta agcatgttgt cctgcatgga
     4321 gtggaccaga tgttggatat gggatttgct gatcaagtgg aagagatttt aagtgtggca
     4381 tacaagaaag attctgaaga caatccccaa acattgcttt tttctgcaac ttgccctcat
     4441 tgggtattta atgttgccaa gaaatacatg aaatctacat atgaacaggt ggacctgatt
     4501 ggtaaaaaga ctcagaaaac ggcaataact gtggagcatc tggctattaa gtgccactgg
     4561 actcagaggg cagcagttat tggggatgtc atccgagtat atagtggtca tcaaggacgc
     4621 actatcatct tttgtgaaac caagaaagaa gcccaggagc tgtcccagaa ttcagctata
     4681 aagcaggatg ctcagtcctt gcatggagac attccacaga agcaaaggga aatcaccctg
     4741 aaaggtttta gaaatggtag ttttggagtt ttggtggcaa ccaatgttgc tgcacgtggg
     4801 ttagacatcc ctgaggttga tttggttata caaagctctc caccaaagga tgtagagtcc
     4861 tacattcatc gatccgggcg gacaggcaga gctggaagga cgggggtgtg catctgcttt
     4921 tatcagcaca aggaagaata tcagttagta caagtggagc aaaaagcggg aattaagttc
     4981 aaacgaatag gtgttccttc tgcaacagaa ataataaaag cttccagcaa agatgccatc
     5041 aggcttttgg attccgtgcc tcccactgcc attagtcact tcaaacaatc agctgagaag
     5101 ctgatagagg agaagggagc tgtggaagct ctggcagcag cactggccca tatttcaggt
     5161 gccacgtccg tagaccagcg ctccttgatc aactcaaatg tgggttttgt gaccatgatc
     5221 ttgcagtgct caattgaaat gccaaatatt agttatgctt ggaaagaact taaagagcag
     5281 ctgggcgagg agattgattc caaagtgaag ggaatggttt ttctcaaagg aaagctgggt
     5341 gtttgctttg atgtacctac cgcatcagta acagaaatac aggagaaatg gcatgattca
     5401 cgacgctggc agctctctgt ggccacagag caaccagaac tggaaggacc acgggaagga
     5461 tatggaggct tcaggggaca gcgggaaggc agtcgaggct tcaggggaca gcgggacgga
     5521 aacagaagat tcagaggaca gcgggaaggc agtagaggcc cgagaggaca gcgatcagga
     5581 ggtggcaaca aaagtaacag atcccaaaac aaaggccaga agcggagttt cagtaaagca
     5641 tttggtcaat aaggatccac cggatctaga taacgcccat cgatagatcc taatcaacct
     5701 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5761 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5821 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5881 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5941 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6001 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6061 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6121 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6181 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6241 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     6301 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6361 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6421 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6481 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6541 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6601 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     6661 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     6721 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7141 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     8761 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8821 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     8881 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8941 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9001 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     9061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9121 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9181 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9241 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9301 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     9361 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9421 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9481 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9541 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9601 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9661 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9721 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9781 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9841 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9901 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9961 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    10021 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    10081 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    10141 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    10201 ggcaag
//
