LOCUS       sequence-355923-       14164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang 
            ZH, Shan L, Yang L, Liu J, Chen LL
  TITLE     lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol 
            I transcription.
  JOURNAL   Science. 2021 Jul 30;373(6554):547-555. doi: 
            10.1126/science.abf6582.
  PUBMED    34326237
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-07-30- 30"; volume: "373"; 
            issue: "6554"; pages: "547-555"
COMMENT     Sequence Label: p23-puro-EGFP-RPA194
FEATURES             Location/Qualifiers
     source          1..14164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             159..792
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    839..964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1461..1694
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1879..1923
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2216..2333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2381..3559
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2612..3550
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3507..3527
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3565..3567,3568..3570,3571..4281)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3565..3567,3568..3570,3571..4281)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3610..3631)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3871..3890)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4218..4239
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9501..10074
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9668..9685)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9895..9914
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10094..10693
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10094..10113)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10590..10610
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10712..11300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10765..10785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11183..11194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11375..11608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11727..11745)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11813..11917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11918..11986,11987..12778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12136..12155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12949..13537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13438..13457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13691..13708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13948..13969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13984..13989,13990..14007,14008..14014)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14022..14038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14027..14049
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14046..14062
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14046..14062
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14112..14131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
       61 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
      121 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
      181 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
      241 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
      301 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
      361 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
      421 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
      481 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
      541 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
      601 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      781 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      841 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      901 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      961 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     1021 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1081 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1141 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1201 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1261 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     1321 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1381 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1441 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1501 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1561 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1621 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1681 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1741 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1801 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1861 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1921 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1981 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2041 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2101 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2161 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
     2221 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2281 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2341 attacaggga cagcagagat ccagtttgga ctagtcgtga ggctccggtg cccgtcagtg
     2401 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2461 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2521 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2581 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2641 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2701 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2761 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2821 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2881 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2941 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3001 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3061 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3121 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3181 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3241 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3301 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3361 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3421 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3481 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3541 tccatttcag gtgtcgtgaa gcggatggtg agcaagggcg aggagctgtt caccggggtg
     3601 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3661 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3721 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3781 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3841 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3901 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3961 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4021 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4081 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4141 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     4201 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4261 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc catgttgatc
     4321 tccaagaaca tgccctggcg gcggctgcag ggcatttcct tcgggatgta ttcggctgaa
     4381 gagctcaaga aattaagtgt taaatccatt acgaaccctc gatacctgga cagcctgggg
     4441 aacccatcgg caaacggcct gtacgattta gctttgggcc ctgcagattc caaagaggtg
     4501 tgctccacct gcgtgcagga cttcagcaac tgttctgggc acctgggcca cattgagctc
     4561 ccactcacag tgtataaccc tctcctcttc gataagctgt acctgctgct tcggggctct
     4621 tgtttaaact gccacatgct gacttgtccc cgggccgtga ttcacctctt actctgccag
     4681 ctgagggttc tggaagtcgg ggccctacaa gcagtctacg agcttgagag aattctgaac
     4741 aggtttctgg aagaaaatcc cgatccctct gcctctgaaa ttcgggagga attagaacaa
     4801 tacacaactg aaattgtgca gaacaacctc ctggggtccc agggcgcaca tgtaaagaac
     4861 gtgtgtgaga gcaagagcaa gctcattgct ctcttctgga aggcacatat gaatgctaag
     4921 cgctgtcccc actgcaagac cgggcgatcc gttgtccgaa aggaacacaa cagcaagttg
     4981 actatcacgt ttccagccat ggtgcacagg acagctggcc agaaggactc tgagcccctg
     5041 ggaattgagg aagctcagat aggaaaacga ggatacttaa cacccaccag tgcccgcgaa
     5101 cacctttctg ccctgtggaa gaatgaagga ttctttctga actacctttt ttcgggaatg
     5161 gatgatgatg gtatggaatc cagattcaat cccagtgtgt tctttctaga tttcttggtg
     5221 gtgccgccct caaggtatcg cccagtcagt cgcctaggag accagatgtt tactaatggc
     5281 cagacggtga acttgcaggc tgtcatgaag gatgtagttc tgattcgaaa acttctggca
     5341 ttgatggccc aagaacagaa gttgccagag gaagtggcca cacccactac agatgaggaa
     5401 aaagactctt tgattgctat tgaccgatcc tttttgagta cacttccagg ccagtccctc
     5461 atagacaaac tttacaacat ttggattcgc cttcagagcc acgtcaatat tgtgtttgat
     5521 agcgagatgg acaaactaat gatggacaag tacccaggca ttaggcagat cctggagaag
     5581 aaagaaggcc tgttccgaaa acacatgatg ggaaagcgag tggactacgc tgcgcgctca
     5641 gtcatctgcc cagacatgta catcaacacc aacgaaattg gaattcccat ggtgtttgcc
     5701 acaaaactga cctacccaca gccagttacc ccatggaatg ttcaggaact taggcaagcg
     5761 gtcatcaacg gccctaatgt gcacccagga gcctccatgg tcatcaatga ggacggcagc
     5821 cgcacagccc tgagcgctgt ggacatgacc cagcgagagg ccgtggccaa gcagcttctg
     5881 accccagcca cgggggcacc taagccccag gggacaaaaa ttgtgtgccg gcatgtgaag
     5941 aatggggaca ttctgctact gaaccgacag cccacactgc acagaccctc catccaggcc
     6001 caccgtgccc gcatcctgcc tgaagagaaa gtgctgcggc tccactatgc caactgcaag
     6061 gcctataatg ccgactttga tggagacgag atgaatgccc atttccccca gagtgagctg
     6121 ggccgggccg aggcctacgt cctggcctgc actgatcagc agtaccttgt tcccaaggat
     6181 ggccaaccat tggcgggact gatccaggat cacatggttt caggggcaag catgactact
     6241 cggggttgct ttttcacccg ggagcactat atggagctgg tgtaccgagg actcacggac
     6301 aaagtggggc gcgtgaagct cctttctcct tccatcctga agccctttcc gctgtggaca
     6361 ggaaaacagg ttgtgtcaac gctgctcata aatataatcc cagaggacca catcccactg
     6421 aacttatctg gaaaggcgaa aatcactggg aaagcctggg tgaaggaaac tcctcgatcc
     6481 gttcctggct ttaaccctga ctcgatgtgc gagtcccagg tgatcatcag ggaaggggag
     6541 ctgctctgcg gagtgctgga caaggcgcac tatgggagct ccgcctacgg cctggtccac
     6601 tgctgctatg agatctatgg aggcgagacc agcggcaagg ttctaacctg cctggcccgc
     6661 ctcttcaccg cctacctgca gctctacaga ggcttcacct tgggcgtgga agacattttg
     6721 gtgaagccaa aggcagatgt caagaggcaa cgtatcattg aagaatccac ccactgcggg
     6781 ccccaggctg tcagggctgc attaaacctg ccagaagccg catcatatga tgaggtccga
     6841 ggaaaatggc aggatgccca tctgggcaag gaccagaggg attttaacat gattgatctg
     6901 aagttcaagg aggaagtgaa ccattacagc aatgagatta acaaggcatg catgcctttt
     6961 ggcctacaca gacagttccc agagaacagc ctgcagatga tggtgcagtc gggagccaaa
     7021 ggttcaactg tgaacacgat gcagatctcg tgcctgctgg gccagattga actggaaggt
     7081 cggagacccc cgctgatggc gtctggcaag tcactgccct gctttgagcc ttatgagttc
     7141 acccccaggg ctggtggctt tgtcactggc aggttcctta ccggcatcaa acctcctgag
     7201 ttcttcttcc actgcatggc aggacgagag ggcctggtgg acactgctgt gaaaaccagc
     7261 cgctcaggct atctccaaag gtgcatcatc aagcacctag aggggctggt cgtgcagtat
     7321 gatctcacgg tccgtgacag tgacggcagt gtggtgcagt tcctgtatgg ggaggatggc
     7381 ctggacatcc ccaagacaca gttcctgcag cccaagcagt tccccttcct ggccagcaac
     7441 tacgaggtga taatgaaatc acagcatctc catgaagttt tatccagagc agatcccaaa
     7501 aaagctctcc accacttcag agctatcaaa aaatggcaaa gcaagcaccc caacaccctg
     7561 ctgagaagag gcgccttctt gagttattcc cagaaaattc aggaagctgt gaaagccctg
     7621 aaacttgaga gtgaaaaccg caatggccgc agccctggga ctcaggagat gctgaggatg
     7681 tggtatgagt tggatgagga aagccgaagg aaataccaga agaaggcggc cgcttgtcct
     7741 gaccccagtc tgtctgtctg gcgtcctgac atctactttg catcagtgtc agaaacattt
     7801 gaaacaaagg ttgatgacta cagtcaagag tgggcagctc aaacagagaa gagttatgag
     7861 aaatcagagc tttctctcga caggttgagg accttgctgc agctgaagtg gcagcgctca
     7921 ctgtgtgagc cgggcgaggc tgtgggcctg ctggctgccc agagcatcgg agagccctcc
     7981 acccagatga ccctcaacac cttccacttt gcaggcagag gcgagatgaa cgtcaccctg
     8041 ggcattccaa ggttgcggga gattctcatg gtggccagcg ccaacatcaa gacacccatg
     8101 atgagcgtgc ccgtgctcaa caccaagaaa gccctgaaga gagtgaaaag cctgaagaag
     8161 caactcacca gggtgtgctt gggggaggtg ttgcagaaaa ttgacgtcca ggagtccttc
     8221 tgtatggaag aaaaacagaa caaattccag gtgtaccagc tgcggtttca gttcctgcca
     8281 catgcatatt accagcagga gaagtgcctg agacccgagg acatcctgcg cttcatggaa
     8341 acaagattct ttaaacttct gatggaatcc atcaaaaaga agaataataa agcatcagct
     8401 ttcaggaacg taaacactcg aagagctaca cagcgggatc tggacaacgc tggggagttg
     8461 gggaggagtc ggggagagca ggagggtgat gaggaagagg aggggcacat tgtggatgct
     8521 gaagctgagg agggagacgc cgatgcctct gatgccaaac gcaaggagaa gcaggaggag
     8581 gaggttgatt atgagagtga ggaagaggag gagagggagg gcgaggagaa cgacgatgaa
     8641 gacatgcagg aggaacgaaa tccccacagg gaaggtgctc gaaagaccca agagcaagat
     8701 gaagaggtgg gcttaggcac tgaggaggac ccgtcccttc ccgccctcct gacgcagccc
     8761 cggaaaccca cccacagcca ggagccccag gggcccgagg ccatggagcg ccgggtccag
     8821 gctgtgcgtg agatccaccc gttcatagat gactaccagt acgacaccga ggagagcctg
     8881 tggtgccagg tgacagtgaa gctccctctg atgaagatca actttgacat gagctccctg
     8941 gtagtatctt tggcccatgg tgccgtcatc tatgcgacca agggcatcac tcggtgcctc
     9001 ctgaatgaaa caaccaacaa taagaacgag aaggagcttg tgctaaacac agaaggaatc
     9061 aacctcccag agctattcaa gtatgcagag gtcctggatc tgcgccgcct ctactccaac
     9121 gacatccacg ccatagccaa cacgtatggc attgaggccg cgctgcgggt gatcgagaag
     9181 gagatcaagg atgtgtttgc cgtgtatggc atcgcggtcg accctcgcca tctctccctg
     9241 gttgctgatt atatgtgctt cgagggtgtt tacaagccac tgaatcgctt tgggatccgg
     9301 tcaaactctt ccccgctaca gcagatgaca tttgaaacca gcttccagtt tctgaagcaa
     9361 gccaccatgc tgggatccca cgatgagctg aggtctcctt ctgcctgcct tgtggtcggg
     9421 aaggtcgtca ggggcgggac aggcctgttc gagctcaagc agcctctgag ataacagcta
     9481 gctcgagtct agaggatctg cccctctccc tccccccccc ctaacgttac tggccgaagc
     9541 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     9601 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     9661 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     9721 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     9781 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     9841 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     9901 tcctcaagcg tattcaacaa tgggctgaag gatgcccaga aggtacccca ttgtatggga
     9961 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
    10021 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc
    10081 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    10141 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    10201 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    10261 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
    10321 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    10381 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    10441 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    10501 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    10561 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    10621 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    10681 gcccggtgcc tgacgcccat cgatagatcc taatcaacct ctggattaca aaatttgtga
    10741 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10801 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10861 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    10921 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    10981 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    11041 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    11101 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11161 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11221 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    11281 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
    11341 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    11401 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    11461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    11521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    11581 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    11641 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
    11701 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    11761 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
    11821 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    11881 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    11941 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    12001 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    12061 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    12121 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
    12181 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    12241 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    12301 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    12361 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    12421 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    12481 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    12541 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    12601 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    12661 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    12721 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    12781 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    12841 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    12901 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    12961 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    13021 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    13081 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    13141 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    13201 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    13261 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    13321 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    13381 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    13441 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    13501 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    13561 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    13621 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    13681 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    13741 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
    13801 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    13861 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
    13921 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    13981 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    14041 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
    14101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    14161 gata
//
