LOCUS       sequence-355932-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmeIF4Ga-Y80A-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7769..7810
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7820..7837
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7860..7877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7910..8044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7934..7953)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7988..8007
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8645..9233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8725..8744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9404..10195,10196..10264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10027..10046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10265..10369)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10437..10455
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgggtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatgc agcaagcaat
     2761 ccctacacta cctactcagt cggacatcga caaggctatg caaccacact cggctcagaa
     2821 catgtacgtg tctacaggta ataataactc aggtaacacg cggtcaaacc cgcagagcgg
     2881 tgggattttc cgtgggcctc catctacacc aaacgctcca aggggagcaa gcggaggggc
     2941 tacgcgacac gtgcacgttc agccaatgta ttcgcagcca ttgcatcaaa atatggttct
     3001 ccagcagtat acgcagtaca atcctcgaca gcagaccttc ccggcatcac acttacagta
     3061 tgccccggct ccgatgccct attatcaata tcagtacgtg cccacattgc aacagcaacc
     3121 tcctcacaca cggagcgccg tgactgtgaa cactaatgtg aacgtaggga ataacctcca
     3181 acccgttcaa tcaggcccaa acggcccatt gccagtccca ggcgccagtt cttctcagat
     3241 acagcttttg acgagtacgg tacaacctgg tgctagtaca gtgatgggag tgggaggacc
     3301 aggcagcacg atgggacagg taggagtccc ccccatggtc ggtgtgggag tgatgactac
     3361 gagcgttcag ccacagccgg tacaagtcca acccgccagt cgtcggcgac accaacaccg
     3421 ccttcaaatc atagacccca caactaaaaa aaatatttta gacgattttg ataagacaaa
     3481 atcaaacaca gacaatgaat tttccgatca ggtaacctcc acaaacacac cagcaactgt
     3541 cctttcagaa ggtccaatac gtattccaca gcaagaaagt gttggtttaa ataatttgac
     3601 cagtacatca tcacaaggat cagaatcacg gacaaacgcg ccttatatcc ccattgaaac
     3661 aacaccaata agtcgaacag atgttgggcc gactccaatc gtatccgcga tgactgatgc
     3721 tccttcggtt gaaatattac caacgcccca aagagggaga agcagaaaaa ttcctatagt
     3781 gtctcctaaa aatgtatctg agtccatagt tgctcctatc actgatgaaa cggatgatgc
     3841 agggtcaaca ccgatatcca gagccactga agaatctatg ccacctaatc agacccatcc
     3901 aaatttactc atatccgatt catcaaaaca taagcaagca gtatcaaata gtgagatttc
     3961 aaaagacgct cctactggtt taaaggaaat gcatgttgct gagctggagt ccagtgtagc
     4021 ctccgaaaat catccagctg gaatactaga tgtcaatgtc aacaagagtt cacaatcatt
     4081 ggatttttct gcagatcccg aaattgatag tattggcgca atatcaccta cgagtccaaa
     4141 cgcagtttct cctattttac acgaagtttt agataatact gagttgtcaa aaaaattaga
     4201 gaattctacg acagaaagat ttaaagatag ccaaagcgta gagaagccaa ctcatcaaga
     4261 actaagttta agaaatgcca cagacgagac tgaaatatcc gcaatggcat tacaagatgt
     4321 gaatagtttg gataataacc aaattgaaaa aacataccct tcaaagccga aactcaatgt
     4381 agatgtttca gaagacattt cctccagaga gtcagcaata aagtcaactt ctacaaaaaa
     4441 tacaggagta gatgtaggtc tccaatcaga ttctaagcca gagactaccc ttaacgataa
     4501 acaggactct actgacctaa aagtgaaagt gtctgcgaaa atttcatcta tcataaacta
     4561 taatgagggt caatggtcac caaacaatcc aagcggaaaa aaacaatacg accgagaaca
     4621 gttattacag ttacgcgagg ttaaggcctc acgtatacaa ccagaggtaa aaaatgtgtc
     4681 tattcttcct caaccaaact taatgccatc atttattcga aataacaata ataacaagcg
     4741 agtacaatcg atggttggta ttattggcaa tcgtagcaat gaatcggcgg gaaattatat
     4801 tggtaaacag atgtcgatgt ctggcgtaca aagtggcggc ggtagaagca gtatgaaggg
     4861 catgatacac gtaaatttat cattaaatca agatgtaaag ttaagtgaaa atgaaaatgc
     4921 atggcgccct cgggtcttaa ataaatctga tggtgatagc gacgcgaaat ctgcactcga
     4981 aaaagatgaa ttggtacgtc gtgttcgggg tattttaaat aagcttaccc cagaaaggtt
     5041 tgacacactt gttgaggaga ttataaaatt gaaaattgat acacccgaca aagtggacga
     5101 agtaattgtg ttggtatttg aaaaggccat tgatgagcca aacttttctg tgtctgctgc
     5161 cagactatgt caacgacttg ctgcggaagt gaaggtaata gacgagcgca tggaaagcga
     5221 aaccaaatct aactcggcac actttcgaaa tgcattgtta gataaaactg aacaggaatt
     5281 cacccaaaac gtttcacaaa gcaccgcaaa ggaaaaaaaa ttacagccaa ttgtggataa
     5341 aattaagaaa tgtactgatg ccaatgaaaa agcggaactt gaggcatttt tggaagaaga
     5401 agaaagaaaa ataagaagac gatccggagg gacagtacga tttattggcg aactttttaa
     5461 gatatctatg ttgactggca aaataatata ctcttgcatc gatacacttc ttaaccctca
     5521 ttctgaagac atgcttgagt gtttgtgcaa gctgcttaca actgtgggtg ctaaatttga
     5581 aaagacccca gtaaattcca aagaccctag tcggtgttat tcgttggaaa aatcgatcac
     5641 caggatgcag gccatcgcat cgaaaactga caaagatgga gccagagtaa gctcaagagt
     5701 acgatttatg cttcaagacg ttatcgactt gcgtaagaat aaatggcaaa cttcgcggaa
     5761 tgaagcaccc aaaactatgg gacaaattga aaaagaggca aagaatgagc agctttctgc
     5821 acaatatttt ggtacacttt ccagtactac acccggagga tcgcagggcg gctctggtaa
     5881 acgagatgac cgtggaaact ctcgatacgg tgaatctcgt tcaagtagcg cgtatggtgg
     5941 cagccacagt caacgtggtg ataatggcaa ccttcgacac cagcagcaga acaacgtcgg
     6001 gggaaacgtc tctggaggtg ctggacactc caatggaaac aatgatgaaa atacttggca
     6061 tgtgcaaacc agcaaaggta gccgctctct agcggttgac agcaataaac tggaaggcct
     6121 gtctaaactt tctgaccaaa atctggaaac aaagaagatg ggtggattaa cgcaattcat
     6181 ttggattagt agtgacacga caagactatc gtcggcgccg acaccaaccc catccaatcc
     6241 tttcgctgtg ctctcatccc taattgataa aaatagtaat gaaagggatc gcgatcgcag
     6301 tggacccaga aacaaaggat catacaacaa aggttctatg gagcgggatc gttatgatag
     6361 aggtatgcat tcgagaactg gatcatctca gggatcacga gaaaactcat cctcccgcgg
     6421 tggtcagcag ggtcgaactt tattgagttc atcagttcaa aaatctacta gtcattcaaa
     6481 atacacccaa caggcaccac ctacacggca tactgttaaa gcccagtcat cagtgggcag
     6541 ctctaacgtt aacacaggtc cactctaccg tggaagtgag cagcaaacat cagcaacctt
     6601 ctcgcagacc acccgttctg ttgctcctgt agcagtgttc atcgaagcta gcgaaacaga
     6661 tttaaaactc atcaaatctg ttgtttcaga aattgtagac ctatcagctg catctaaaga
     6721 agtcacgccc ggtgcagttt cgtgcataaa acgagtacca gagaaactac ggtgcagttt
     6781 catttattat attttaacag attatttgca tttagcaaat gtaggtaaac agtacaggcg
     6841 gtatttatct attgccgtct cccaacttat tcagcagaat tacatttctg ccgatcattt
     6901 gagactggct tacaacgaat ttaccgtata tgcaaacgat ttgattgttg atattccgga
     6961 gctttggcta tacattcttc aatttgccgg gccactaatt gtaaagaaaa tattgactat
     7021 atcagatttg tggaacaaca atttaaaaga aaatagtcca tcaaacgtcg ctaaaaagtt
     7081 ccttaaaaca tacttgatat attgcacaca agaagttgga ccaaattttg ctcggaatat
     7141 gtggatcaag tttaacctaa aatggtcaga ttttatgcct gaaagtgaag ttgccgactt
     7201 tattaaattt aacagactgg aatatgtgga aaacgaatca aagtcgccag tgattgatca
     7261 tagagagact ccagaaaagc acgttaaaaa tgtgatagac catattgagc acttactaaa
     7321 ggaaggaaca acggcggact gcattattga ttatagtaac ggaaatataa tggtggttga
     7381 taagttgttt atcagaggat tgacggaaac attgagtaat ttctcgattc actacaagga
     7441 caacagttac aagcttgagt ccgaaacctt tcaaaagttt tgcataccgg ttttgcagag
     7501 atacattgat tctaatgaag accaccaact tgaatgttta tatactttgc agcttttagt
     7561 tcatggattg gagcatccaa gagggttgct gagtgaacta attggcgagc tatatgatgc
     7621 ctttgtaata cagaaggaat cgctttgtaa gtggcgagat tcaaaggatc agtctgctgg
     7681 caagggcgtt gctgtaaaaa gtcttaatcc tttttttaac tcgttgttaa acgatgatgc
     7741 caaccctcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     7801 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     7861 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     7921 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7981 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     8041 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     8101 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga
     8161 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     8221 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     8281 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     8341 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     8401 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     8461 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     8521 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     8581 atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8641 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8701 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8761 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8821 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8881 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8941 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9001 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9061 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     9121 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9181 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9241 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9301 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9361 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9421 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9481 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9541 cgcgagaccc acgctcaccg gctccggatt tatcagcaat aaaccagcca gccggaaggg
     9601 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9661 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     9721 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9781 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9841 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9901 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9961 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10021 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10081 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10141 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10201 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10261 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10321 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10381 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    10441 ggcgtatcac gaggcccttt cgt
//
