LOCUS       sequence-355933-        6432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSKI8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSKI8_AA
FEATURES             Location/Qualifiers
     source          1..6432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2453..2518
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2543..2569
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3606..3647
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3657..3674
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3697..3714)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3747..3881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3771..3790)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3825..3844
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4482..5070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4562..4581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5241..6032,6033..6101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5864..5883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6102..6206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6274..6292
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(6356..6432,1..2433)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc attaaagctg
       61 gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag aaagaagcga
      121 tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa cgagctgatc
      181 tagtatctct ccacaatcca atgccaactg accaactggc catattcgga gcaatttgaa
      241 gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc accgttaccc
      301 ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg tctctcttca
      361 tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg aagaatgaga
      421 ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca tgttatctat
      481 agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata tacctttaat
      541 gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat attttaagat
      601 gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg catgtgcaat
      661 tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt ttcttggctt
      721 attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc ggcaaaacgg
      781 tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc gtggcggggt
      841 ctgtggctgg gcgcgacgtc accgacgttg gagccactcc tttgaccatg tgtgcgtgtg
      901 tgtattattc gtgtctcgcc actcgccggt tgtttttttc tttttatgct gcgctctctc
      961 tagcgccatc tcgcttacgc atgctcaacg caccgcatgt tgccgtttcc ttttatgcgt
     1021 cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa acgctaaaat
     1081 aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga tagcaacaaa
     1141 agcaacaaca caggtggctt tccctctttc actttttgtt tgcaagccgc gtgcgagcaa
     1201 gacggcacga ccggcaaacg caattacgct gacaaagagc agacgaagtt ttggcgaaaa
     1261 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1321 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1381 aattaggaat cggacgctta tcgttagggt aacaacaaga aatgcttact gagtcacagc
     1441 ctctggaaaa ctgccgcaag ccagagagag agagaaaaag agggagagca gcttagaccg
     1501 catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt tgcgcaaacg catagactgc
     1561 actgaaaaaa tcgattacct attttttatg aatgaatatt tgcactatta ctattcaaaa
     1621 ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc gatgcaacga
     1681 aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata gaaatgcttc
     1741 ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa aaccggctta
     1801 agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg ttgcacaaaa
     1861 tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta atatgaatat
     1921 tatttaactg taatgtttta atatcgctgg acattactaa taaacccact ataaacacat
     1981 gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt aaaagcaccg
     2041 tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac ccccaaattg
     2101 aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg atatacaaga
     2161 cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg
     2221 gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga gcggagagca
     2281 ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc gggaccagtt
     2341 ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga cacaaagccg
     2401 ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacca acatggacgc
     2461 acaaacacga cgacgtgagc gtcgcgctga gaaacaagct caatggaaag ctgcaaacgg
     2521 tggatctcct ccacatatgg cttacccata cgatgttcca gattacgctc ctccaagctt
     2581 gaattctatg ttttctgtcc ttcataaaga ggaaaatgcg cacgacagtc agctatgggc
     2641 gtgcacctgg ggacgcgaca ctgcggcctc agatccggat gacgcggtcg tggagcccga
     2701 ggagaatccc tttgatttcg ataagaagga ggcccgcccg aaggatttct tggtcaccgg
     2761 cggactggat gacttggtaa aggtgtggga cttgcaggag gacaacaccc taaagttgcg
     2821 ccacaaacta aaaggacatg ccctgggagt tgtttctgtg gccgtcagtt ccgatggaca
     2881 gaccattgcc agtagttctc tggactccac catgtgtttg tgggatgccc gttcgggcga
     2941 caagaaacac ctacttagct ttgggcctgt tgatctctgg acggtgcaat tttcaccctg
     3001 caacaaatat gtgatatccg gactgaacga cggcaaaatc tccatgtaca gtgtggaaac
     3061 cggaaaggca gagcagacat tggatgccca aaatgggaaa tacacgctta gcattgccta
     3121 cagtcccgat ggcaagtaca ttgccagtgg agccatcgat ggtatcatta ccattttcga
     3181 tgtggccgcg ggcaaggtag tgcaaacatt ggaaggacac gcgatgccag tgcgtagcct
     3241 ttgcttttct ccgaactcgc agctgctgct cactgcctca gatgatggtc acatgaagct
     3301 ttatgatgta acccactccg acgttgtggg caccctatct gggcacgcat cttgggtgct
     3361 gtgcgtggca ttctccgagg acggcaaaca ctttgccagc tcctccagtg ataacagcgt
     3421 gaaaatatgg gacacctcgg aacgcaagtg cctgcacacg ttcgccgagc acacagatca
     3481 ggtgtggggc gtccgatata gccccggaaa cgacaaggtg gcctctgctt ctgaggacaa
     3541 gtccctgaac atatactact gtccgcccaa tgccattgtt tgatctagag ggcccgcggt
     3601 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
     3661 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct
     3721 gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg
     3781 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3841 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3901 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3961 acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca
     4021 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     4081 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     4141 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     4201 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     4261 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     4321 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     4381 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggccag
     4441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4921 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5101 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5161 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5221 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5281 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5341 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5401 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5461 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5521 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     5581 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5641 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5701 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5761 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5821 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     5881 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5941 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6001 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6061 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     6241 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6301 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
     6361 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
     6421 atcatttttc ca
//
