LOCUS       sequence-355935-        8613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8613)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUNR_AA
FEATURES             Location/Qualifiers
     source          1..8613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5864..5905
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5915..5932
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5955..5972)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6005..6139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6029..6048)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6083..6102
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6740..7328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6820..6839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7499..8290,8291..8359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8122..8141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8360..8464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8532..8550
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttgat gaatacccaa tcgaaggtgt accgtaccgg cgaagaaatc
     2701 tatgacaact tgccatgcga tagttatttc aacatgaatg ccattcgcaa cctgggaatc
     2761 ccaacaacgt ttccgaccat tggaacattt acacttgact ctactacgtt gggactgcag
     2821 ccgcagggtc agggcccgtc gccccagcag cagcagcatc aacagcaaca acaacagcag
     2881 cagcagcaac atcagcagaa tatgcaccac catcaacatc agcagcaaca catgcagcag
     2941 cagcagcaac aacaacaaca tcagcagcaa caccaacagc agcaacatca acaacaacag
     3001 caacatcagc agcagcagca gcagcaacaa cagcatccaa ccattggaat gttcgatgcc
     3061 aacgaggtta acgatgtcat ccagaatcct cctcaaatcg gggtattcca gtctaacagc
     3121 gttctgacca acggtgccgg cagtggctcc tcgatctttg gctcccaatc gagcaattca
     3181 tcggcggcag ctgcagatcc cagccagacc acccgggaaa ctggcatcat tgaaaagttg
     3241 ttgcattcgt atggattcat tcagtgctgt gagcggcagg cgcgtctttt ctttcatttc
     3301 tcgcaattca gcggtaatat tgatcatttg aaaatcggag atcccgtgga gtttgagatg
     3361 acctatgatc gccgcaccgg caagccgata gctagtcaag tatcaaaaat agccccagag
     3421 gttgttctct ccgaggagcg ggtcaccggc acggtgacca ccgagctgcg caccgatagc
     3481 gccaacaatg tgcttaactc cagcgagacc acaggtcgca taagctatga gaaccgaggc
     3541 gaatgcttct ttttacccta taccaaagac gatgtggaag gtaatgtcaa cttgcgcgct
     3601 ggcgacaagg tcagctttca aatcgcaacg aaccaaagag gtaatctagg cgcctgccat
     3661 attcgcctgg aaaatccggc ccagccggtt aagtatcgtg gggttgtctg ctcgatgaag
     3721 gagtcgtttg gctttatcga acgtgccgat gtggtcaagg agattttctt ccacttctca
     3781 gaggctgagg gcaatgttga gctgcgtccc ggtgacgatg tcgagttcac catccagacg
     3841 cggagcgtaa gtggcagtgg cgatccgacc aatcccaata tgctccaatc aaaaatgcag
     3901 tctgcatctg tgcctccgca gggccgtgaa tttgcgtgca acatcacgcg cctagctccg
     3961 ggatcagtga tctttgagga cgtcgatagc acggtgtata agggccaggt gcttaagtcg
     4021 cttgatcgca acaacccggt gcgccagaac aatgatccct tgccgggtcg tattcgttat
     4081 agggccttgg attactccga agtggaggtt cctttcgggg acaaggatca aaagggtgac
     4141 ttcaccctgc gccacggcga ttgggtgcag ttcctgcttg ccaccgatcg gcgtgatcaa
     4201 ttgcagaggg ccacctccat tgccctgttg gacgagactt tcaaggtttc cggcgagaag
     4261 cgggagcagg gcaccattgc ctctctcaag gagggcttcg gattcctgcg ctgcgtagag
     4321 cgtcaggctc gtttattttt ccactttact gaagttttag atacgagccg cgaaatcgat
     4381 atcaatgatg aggtagaatt cactgtcatt caggagcctg gattggccta taataactcg
     4441 cgtttgcagg ccattcgcat taaacacttg ccgcccaact cagtgcaatt tgaaactcta
     4501 gtggccagca acatagaagg atgtgtaact cgcgaggcgc ctaagagccc aattaaatct
     4561 caagatcgcg tcgagggcgg tgtgattacc tatgaacatg ccgatgttaa aaagactata
     4621 atgtattttc ttaaagactg cgaaaaacca ccaaggattg gggagcgcgt acgcttcgat
     4681 atttacatgg tcaaacgtaa caaggagtgt atagccgtca acgtacagca agtatccctg
     4741 catcagcagc aacaacagca gcaacagcag ttgcatctta atcagtcgaa tgctggggca
     4801 aatatcaacc aaaatgacca gctgggagga ttgtcgaatg gaatcagtag cagcagctcg
     4861 aatgcttcgc tacagaatgg ctatgtcatg cacggcagcc ctggcggcag caccagcagc
     4921 gtgggcagca acaatccggt tcatctggat gaattcaaga tggagaacaa caaccatgcc
     4981 ggatccgatg ccggtcaggt gtaccgtggc ttcatagccg ttatgaagga gaactttgga
     5041 ttcattgaga ctttgtccca cgatgaggag gtcttctttc actttagcaa ctatatgggc
     5101 aatcccaatt ggttggagct gggtcaggag gtggagtaca ctcttgctcg taatgggaat
     5161 acatccgttt ctggaaactg tctgcctgcg gaaaatgtcc gtatgctgcc aaagaactct
     5221 attccccagc cggctgtgct ggaaaccaca cacaatggcg tggtagcacg tccactgcgt
     5281 tgcatcaatc ccgaccagca ggaatatgcc ggtctcattg aaattctcga tgagctacgt
     5341 accactgtca tttcgcaaca cgaattcggg attactagtc tggtgaacaa gagggatctg
     5401 ctccagaagg gcgatctcgt tagcttccgc atcgatgaga gtggtcgagc agcatgcgtg
     5461 aatgccgtaa ggcagaagaa gcgtgccact gtggactcca tcaagggtca gtttggtttt
     5521 cttaactttg aggtggagga cggcaaaaaa ctgttcttcc acatgtctga ggtgcagggc
     5581 aatactgtcg ctttacatcc aggcgatacc gtggagttct ccgtggtcac caatcagcgc
     5641 aatggaaagt cgtcggcttg caatgttctg aaaataaacg atcgtccgga tcgtctgatc
     5701 tcgcgcctta agctcaacgg cgatgacact gtgccgcgac tgatcctcat tcgcgcaccc
     5761 aagggaccgc agggcaaggg cttttccgtt ctagctcgtc atccacgcat tccaggcaac
     5821 ttggtggagt agccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     5881 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     5941 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     6001 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6061 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6121 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6181 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6241 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6301 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6361 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6421 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6481 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     6541 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     6601 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     6661 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     6721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7141 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7321 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7501 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7561 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7621 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7681 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7741 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7801 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7861 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7921 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7981 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8041 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8101 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8161 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8221 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8281 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8341 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8401 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8461 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8521 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attctatatt
     8581 ctaaaaacac aaatgatact tctaaaaaaa aat
//
