LOCUS       sequence-355936-        7979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot3-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7979)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q
FEATURES             Location/Qualifiers
     source          1..7979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5285..5326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5336..5353
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5376..5393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5426..5560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5450..5469)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5504..5523
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6161..6749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6241..6260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6920..7711,7712..7780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7543..7562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7781..7885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7953..7971
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg attgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgatggctg cgacgagaaa attgcaaggc gaaattgatc gatgtttaaa
     2761 aaaggtagca gagggcgttg aaacgtttga ggatatttgg aaaaaagtac acaatgcgac
     2821 aaacaccaat cagaagcaaa aacatctgca ggagaagtat gaggcagacc tcaaaaagga
     2881 aatcaaggaa ttgcaacgac ttcgcgacca aattaaatca tggattgcat cagctgaaat
     2941 taaggataag agctcccttc ttgagaatcg acgtctcatt gaaacacaaa tggaacgctt
     3001 taaggtggtt gagcgcgaga cgaaaacaaa agcctactcc aaggaaggct tgggggctgc
     3061 acaaaaaatg gacccagctc aacgcatcaa ggatgatgcg cgtaactggc taacgtccag
     3121 tatttcgagc ttgcagattc agatcgatca atacgaatcc gaaattgaga gcctgctggc
     3181 cggaaagaaa aagcgcctag accgtgataa acaagaacgt atggacgatc ttcgcggcaa
     3241 actggatcgt cacaaattcc atattaccaa attggagaca ttgctgcgat tgttggataa
     3301 cgatggagtg gaggctgaac aagttaataa aattaaggat gacgtggagt attatatcga
     3361 cagtagccag gaacccgatt tcgaagagaa cgaatttatt tatgacgaca ttatcggatt
     3421 ggacgaggtc gaactttcgg gaaccgccac gaccgattcc aataattcca atgaaacctc
     3481 cggaagtccc tcctcggtga cgagcggcgg atcgcccagt caaagcccag tgaccgtcca
     3541 gcagatccta aatacaagca gccaaggtgc tgcctcgtcc ggctcctccg ctgcctccgc
     3601 tgcgttgttc caacaacaat tgaccgccgc tcagtccaac ggaaataacg tcggatatgc
     3661 ctccgatacg tcggctgcct catcctcagc caccacctca accgacccag ccggaggaac
     3721 ggtcgccgtt aactgcgtcg gcggtcttgc tgacaagcgt aataagagca gcgaaagcaa
     3781 tgccctaaaa ttgaaaccac aacctcatca acttataaaa cctacccctg tacgagccac
     3841 ggccaagctg cctctgagca gtgataccca agtcaataag attgtcagtt caactcccag
     3901 caaaaatcag caacagctgc ctacagcagc ttcaatcgtt gcaacctccg ctatgcaatc
     3961 gcaaagcagt atcggaagtt gcagctccac tggaggaacg ggagcatctc aatcagcttc
     4021 tagtggtaac aatcctggta acaatcctgc ggttcagcca aatgctccaa cgcctggtca
     4081 aagcggcatt gccgctgcag cagcttcgac taatgttgtt tcggcgacga ttgtgagcag
     4141 cgccaatgtt caaggccaat ctgtgatcca accaacacca acaattgcat ttgctgcagt
     4201 agcaaaacac aatacatcac tcctggaaaa tggtcctgtt ttgcaacagc aactagcagt
     4261 aactcctact gtggcagcta tcgtaggagc tggaacgcag gcgcagcaaa aacatgtgcc
     4321 accattatcc aatcttcaaa caaattcccc tcacatacaa aatggtcttc ctgtctcgga
     4381 cagtactaat gacaacagct gcaatgtcgt cgatacgatt tctctaaaaa caatggcaca
     4441 ggatgctatt aatcgatctg cgatcgatcc taattcactg aatcagcagc aaacaagcag
     4501 catcgatctg agacagccgc agtcgcagaa atcacttctc caacatttca actctgaaac
     4561 caacacgaat caacagcaat tgacatcgca gcagcagcaa cagcttcaga ataactccct
     4621 tgctgcgacc acaggttcta acaacggaac ttcaactggt agtggtctca tgaatgtagc
     4681 aaacgctact ggtcagccta tcagcggcaa tgccaaaacg catgcgtgcc aaccccaaca
     4741 gatggccacg acggaagcgc acattcccac actgcttggt gtgacaccac ttgggccaac
     4801 accacttcaa aaggagcacc aaatgcagtt ccaaatgatg gaagctgcct actatcacct
     4861 gccacagcct atggacacgg aaaagctaca aacgtacttt catcgcgcac cagtgctcac
     4921 gccatcgcac tatccccagg cacagatgcc aatatatgat acagttgagt tttatcaacg
     4981 actctcaacg gagacccttt tttttgtgtt ctactatatg gagggctcca aggcccagta
     5041 tctagcggcc aaggccttaa aaaagcagag ctggcgtttc cacacaaagt atatgatgtg
     5101 gtttcaaagg cacgaggagc cgaagattat caacgatgat tacgaacagg gtacgtacat
     5161 ctattttgat tatgaaaagt ggagtcagcg aaagaaggag ggattcacct ttgaatacaa
     5221 gtacttagag gacaaggagc tgaattgagg cggccgctcg agtctagagg gcccgcggtt
     5281 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca
     5341 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctaaggcctg
     5401 agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg atgagtttgg
     5461 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5521 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     5581 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     5641 caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc ttcaacccag
     5701 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     5761 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
     5821 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
     5881 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
     5941 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
     6001 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
     6061 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc
     6121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6601 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7201 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     7261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7501 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     7561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     7921 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
