LOCUS       sequence-355937-        6239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmBancal-del192-424.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmBancal-del192-424-V5His6_AB
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2453..2518
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2543..2569
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3413..3454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3464..3481
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3504..3521)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3554..3688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3578..3597)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3632..3651
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4289..4877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4369..4388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5048..5839,5840..5908))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5671..5690
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5909..6013)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6081..6099
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(6163..6239,1..2433)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc attaaagctg
       61 gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag aaagaagcga
      121 tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa cgagctgatc
      181 tagtatctct ccacaatcca atgccaactg accaactggc catattcgga gcaatttgaa
      241 gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc accgttaccc
      301 ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg tctctcttca
      361 tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg aagaatgaga
      421 ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca tgttatctat
      481 agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata tacctttaat
      541 gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat attttaagat
      601 gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg catgtgcaat
      661 tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt ttcttggctt
      721 attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc ggcaaaacgg
      781 tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc gtggcggggt
      841 ctgtggctgg gcgcgacgtc accgacgttg gagccactcc tttgaccatg tgtgcgtgtg
      901 tgtattattc gtgtctcgcc actcgccggt tgtttttttc tttttatgct gcgctctctc
      961 tagcgccatc tcgcttacgc atgctcaacg caccgcatgt tgccgtttcc ttttatgcgt
     1021 cattttggct cgaaataggc aattatttaa acaaagatta gtcaacgaaa acgctaaaat
     1081 aaataagtct acaatatggt tacttattgc catgtgtgtg cagccaacga tagcaacaaa
     1141 agcaacaaca caggtggctt tccctctttc actttttgtt tgcaagccgc gtgcgagcaa
     1201 gacggcacga ccggcaaacg caattacgct gacaaagagc agacgaagtt ttggcgaaaa
     1261 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1321 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1381 aattaggaat cggacgctta tcgttagggt aacaacaaga aatgcttact gagtcacagc
     1441 ctctggaaaa ctgccgcaag ccagagagag agagaaaaag agggagagca gcttagaccg
     1501 catgtgcttg tgtgtgaggc gtctctctct tcgtctctgt tgcgcaaacg catagactgc
     1561 actgaaaaaa tcgattacct attttttatg aatgaatatt tgcactatta ctattcaaaa
     1621 ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc gatgcaacga
     1681 aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata gaaatgcttc
     1741 ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa aaccggctta
     1801 agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg ttgcacaaaa
     1861 tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta atatgaatat
     1921 tatttaactg taatgtttta atatcgctgg acattactaa taaacccact ataaacacat
     1981 gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt aaaagcaccg
     2041 tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac ccccaaattg
     2101 aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg atatacaaga
     2161 cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg
     2221 gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga gcggagagca
     2281 ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc gggaccagtt
     2341 ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga cacaaagccg
     2401 ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacca acatggacgc
     2461 acaaacacga cgacgtgagc gtcgcgctga gaaacaagct caatggaaag ctgcaaacgg
     2521 tggatctcct ccacatatgg cttacccata cgatgttcca gattacgctc ctccaagctt
     2581 gatcatgaca aagagactga gaatgaagcg tgaaatgaac gatgaagggg agggaccaca
     2641 ggaccagaag cgcaatcgcc gcaacgagga gaccgttcgc atcctgattc cgagcagtat
     2701 tgctggggct gtcattggca aaggaggaca gcacattcag aagatgagga ctcagtataa
     2761 agccactgtg tctgtcgacg attcccaagg ccccgaacga accatccaaa tatcggcgga
     2821 tatagagtcc acattggaga ttatcaccga aatgctgaaa tactttgagg aacgcgacga
     2881 ggactttgat gtgcgtctac ttatacacca gagcttggcc ggctgtgtca ttggcaaagg
     2941 tggacaaaag atcaaggaga tccgcgatcg catcggctgc cgctttttga aggtcttctc
     3001 gaatgtggca ccacagagca cagatcgagt ggtgcagacc gtgggcaagc agagccaggt
     3061 catcgaagcg gtgcgtgagg tgatcacact tacacgggac actcccatca agggggcgat
     3121 acataactat gatcccatga actttgaccg cgtatatccc aacaacagca cacaggtcac
     3181 cattccaaaa gagctggctg gtgccatcat tggtaaggga ggtggccgca tccgtcgcat
     3241 ccgcaacgag tccagtgcgt acatcaccat cgacgagccc ctgccaaact cgaacgatcg
     3301 tatcatcacc atctcgggca cgccgaagca aatacaaatg gcccagtatc tgctgcaaca
     3361 gagcgtacac gagaatggca ggcgaaacat tctagagggc ccgcggttcg aaggtaagcc
     3421 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     3481 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     3541 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     3601 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3661 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3721 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3781 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     3841 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     3901 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     3961 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     4021 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     4081 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     4141 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     4201 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga
     4261 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4321 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4621 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4681 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4741 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4801 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4861 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4921 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4981 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5041 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5101 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5161 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5221 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5281 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5341 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5401 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5461 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5521 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5581 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5641 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     5701 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5761 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5821 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5881 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5941 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6001 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6061 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa
     6121 ttctatattc taaaaacaca aatgatactt ctaaaaaaaa atcatgaatg gcatcaactc
     6181 tgaatcaaat ctttgcagat gcacctactt ctcatttcca ctgtcacatc atttttcca
//
