LOCUS       sequence-355939-        9249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Surrogate reporter plasmid for the assay of targeted RNA editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu 
            Z, Bao Y, Zhao Y, Wei W
  TITLE     Programmable RNA editing by recruiting endogenous ADAR using 
            engineered RNAs.
  JOURNAL   Nat Biotechnol. 2019 Sep;37(9):1059-1069. doi: 
            10.1038/s41587-019-0178-z. Epub 2019 Jul 15.
  PUBMED    31308540
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-019-0178-z"; journalName: "Nat Biotechnol"; date: 
            "2019-09"; volume: "37"; issue: "9"; pages: "1059-1069"
COMMENT     Sequence Label: Reporter-2
FEATURES             Location/Qualifiers
     source          1..9249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2..21)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     498..518
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    649..1237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(702..722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1120..1131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1324..1557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1629..1750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1666..1685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1720..1739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1790..1925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1945..1964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1946..1964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1974..1991)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1974..1990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1983..2005)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2132..2587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2219..2238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2429..2450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2613..2717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2718..2786,2787..3578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2936..2955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3749..4337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4238..4257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4491..4508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4625..4646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4661..4666,4667..4684,4685..4691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4699..4715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4704..4726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4723..4739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4723..4739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4758..4778
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4760..4778
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4806..5032
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5033..5213
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5260..5385
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5878..6111
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6296..6340
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6607..6723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6746..7049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7050..7253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7204..7224
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7250..7274
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7344..8051
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7489..7507)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7757..7776
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(8151..8153,8154..8867)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8151..8153,8154..8864)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8193..8214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8454..8473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8801..8822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8930..8950)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8948..9245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9115..9249,1)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
       61 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
      121 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      181 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      241 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      301 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
      361 gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg
      421 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
      481 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
      541 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
      601 agctaagcac ttcgtggccg aggagcagga ctgagaattc cagtcgacaa tcaacctctg
      661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
      781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
      841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
      901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
      961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     1021 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     1081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     1141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     1201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     1261 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     1321 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     1381 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1441 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     1501 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     1561 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     1621 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1741 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     1801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     1921 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     1981 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2041 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2101 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2161 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2221 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2281 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2341 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2401 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2461 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2521 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     2581 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4501 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4621 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4741 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     4801 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     4861 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     4921 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     4981 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     5041 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5101 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5161 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     5221 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     5281 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     5341 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     5401 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     5461 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     5521 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     5581 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     5641 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     5701 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     5761 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     5821 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5881 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5941 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     6001 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     6061 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     6121 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     6181 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     6241 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     6301 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     6361 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     6421 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     6481 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     6541 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     6601 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     6661 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     6721 tttatcgata agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6781 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     6841 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     6901 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6961 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     7021 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     7081 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     7141 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     7201 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     7261 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     7321 gactctagag gatccggact agtatggtga gcaagggcga ggaggataac atggccatca
     7381 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     7441 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     7501 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     7561 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     7621 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     7681 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     7741 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     7801 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     7861 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     7921 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     7981 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     8041 agctgtacaa gctgcagggc ggaggaggca gcggcggagg aggcagcggc ggaggaggca
     8101 gcagaaggta tacacgccgg aagaatctgt agagatcccc ggtcgccacc gtgagcaagg
     8161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     8221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     8281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     8341 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     8401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     8461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     8521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     8581 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     8641 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     8701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     8761 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     8821 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaggc gcgccggtgt
     8881 acaccctgca ggggtttaaa cccacgcgtc gaccagtggt cgaccctgtg gaatgtgtgt
     8941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9181 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9241 tttggaggc
//
