LOCUS       sequence-355947-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Surrogate reporter plasmid for the assay of targeted RNA editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu 
            Z, Bao Y, Zhao Y, Wei W
  TITLE     Programmable RNA editing by recruiting endogenous ADAR using 
            engineered RNAs.
  JOURNAL   Nat Biotechnol. 2019 Sep;37(9):1059-1069. doi: 
            10.1038/s41587-019-0178-z. Epub 2019 Jul 15.
  PUBMED    31308540
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-019-0178-z"; journalName: "Nat Biotechnol"; date: 
            "2019-09"; volume: "37"; issue: "9"; pages: "1059-1069"
COMMENT     Sequence Label: Reporter-1
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             30..629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(30..49)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     526..546
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    677..1265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(730..750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1148..1159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1352..1585
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1657..1778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1694..1713)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1748..1767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1818..1953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1973..1992)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1974..1992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2002..2019)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2002..2018)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2011..2033)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2160..2615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2247..2266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2457..2478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2641..2745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2746..2814,2815..3606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2964..2983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3777..4365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4266..4285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4519..4536
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4653..4674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4689..4694,4695..4712,4713..4719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4727..4743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4732..4754
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4751..4767
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4751..4767
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4786..4806
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4788..4806
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4834..5060
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5061..5241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5288..5413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5906..6139
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6324..6368
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6635..6751
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6774..7077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7078..7281
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7232..7252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7278..7302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7372..8079
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7517..7535)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7785..7804
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(8161..8163,8164..8877)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8161..8163,8164..8874)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8203..8224)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8464..8483)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8811..8832
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8940..8960)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8945..9259,1..15)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9125..9259,1)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ctaggctttt gcaaaaagct atcgctagca tgaccgagta caagcccacg gtgcgcctcg
       61 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
      121 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
      181 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
      241 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
      301 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
      361 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
      421 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      481 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      541 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
      601 ggtgcatgac ccgcaagccc ggtgcctgag ctaagcactt cgtggccgag gagcaggact
      661 gagaattcca gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
      721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
      781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
      841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
      901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
      961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     1081 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     1201 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     1261 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     1321 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     1381 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     1441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1561 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     1621 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     1681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     1801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1921 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     1981 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2041 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     2161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2521 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2581 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     2641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2881 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2941 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3001 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3061 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3121 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3181 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3241 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3301 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3361 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3421 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3481 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3541 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3601 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4441 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4501 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4561 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4621 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     4681 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     4741 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     4801 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     4861 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     4921 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     4981 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     5041 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     5101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5221 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     5281 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     5341 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     5401 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     5461 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     5521 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     5581 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     5641 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     5701 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     5761 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     5821 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     5881 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     5941 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     6001 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     6061 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     6121 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     6181 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     6241 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     6301 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     6361 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     6421 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     6481 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     6541 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     6601 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     6661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     6721 tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca
     6781 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6841 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6901 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6961 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7021 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7081 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7141 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7201 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     7261 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     7321 cgctgttttg acctccatag aagacaccga ctctagagga tccggactag tatggtgagc
     7381 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     7441 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7501 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     7561 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     7621 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7681 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7741 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     7801 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     7861 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     7921 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     7981 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     8041 ggccgccact ccaccggcgg catggacgag ctgtacaagc tgcagggcgg aggaggcagc
     8101 ggcggaggag gcagcggcgg aggaggcagc gcctgctcgc gatgctagag ggctctgcca
     8161 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     8221 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     8281 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     8341 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     8401 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     8461 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     8521 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     8581 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     8641 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     8701 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     8761 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     8821 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaggc
     8881 gcgccggtgt acaccctgca ggggtttaaa cccacgcgtc gaccagtggt cgaccctgtg
     8941 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9001 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9121 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9181 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9241 aggaggcttt tttggaggc
//
