LOCUS       sequence-355949-        7727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat-del500-684-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-HA-DmHPat-del500-684-dsRNAres_H
FEATURES             Location/Qualifiers
     source          1..7727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2536..2562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4978..5019
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5029..5046
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5069..5086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5119..5253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5143..5162)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5197..5216
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5854..6442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5934..5953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6613..7404,7405..7473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7236..7255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7474..7578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7646..7664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggcttaccc atacgatgtt ccagattacg ctcctccaag cttgaattct
     2581 gcagatatca tggatgactc gtttttcggc tttgacacga atctaccgga cgaagatggt
     2641 ggcgatggac gcatcgtcga ggcggaatac gatgccctca atgacgaaac ctttggatcc
     2701 gctataaatg gcgactggga ggaagcccat gagacgatgg tacgcctggg cgggaatggc
     2761 gaaagggtgc ggaagcgacc cgcggaatct agtggaaata cggattttgc cgatcaggga
     2821 aatggtgcct tcctgcgaca cgctaatccg ctatcatcgg cgccctcttc aagcggctcc
     2881 actccggccc ctttgaatgc ccccttccga cagtctcgtc acatagggga ctcggacttg
     2941 gagctaaaca tttcctccat gaagttggac gacatggacc tgtcctcttt caccactgat
     3001 agcgaagccg gtgggctgag caaccgcatt aagctggact ccggggtctg gggatcacag
     3061 ccgttcccca acaaccaaca tttgttccgc gagcctatgc gcagcgcctt caagccacag
     3121 cagcagcaac atcagcagca ccaaccacaa caaatgaagc caccgcagga agcgaatgcc
     3181 aactttgcac tgaatccaca gccttttcca aaaattacaa ctctcgagga tatagagcgc
     3241 aatatgatca ttcagcaggc cattccgaaa caacagcagc agagccagca acaggcggaa
     3301 cgcaaaatgt ttgatgattt tagtctgggt aacaggcagc aggtgcctac accaaccatg
     3361 ttgcagcaac agatgttgca gcagcagcat catcagaaac aacagtcgca gcagcagccg
     3421 ccacaacaag cgcagcacaa ggtaccccct ggcttcttgg gcacgccgca cacctctcca
     3481 ccgaggccaa atttgggttt ccccggaccc gagctactgc ccacgccatc gtcgcagcaa
     3541 caactaaacg gtattggtgg caaccgagtg ccgcccggat ttatttaccc gcaaggtctg
     3601 cccgggaata ttccgcaatt gccccagcat cctctaatgc agcagcacat gccgcacaac
     3661 tttccactgc ccggcggcaa ccagcgacct ctacccaatc cacaagctct gcccacggcc
     3721 ctgaacaatt tcgcgatgca tccaagcttc aatgcgatgc gcgccgctgg aatccatcca
     3781 ggggctttta tgcaacctcc ccatccgcac cagatgctgc caccgcgaat gccgccgcac
     3841 ccgctgcaag ctgccacaag tctattaggc cagcagccac cgaattccat gtataacatg
     3901 ttcaacatgc gactagtgca agagatccag caaaaccatc cgctgcttca acaggctgct
     3961 gttgtacgcc aaatgcaaca gagcagagca ggatccgtgg ccagcgatcg tcagagatcg
     4021 caggcgcaac agcagcaaca aggacgacgt ccagatggta caggaaatta cccgctggaa
     4081 gagtaccgga agtcgcgaca cattttgctt ctgattgaaa cactgtatcg ctatctgctg
     4141 aagttggagg atttggaaag cccagaagtg atggccacca ttgagctgaa aaagaaaaag
     4201 gaagccgaac ggatcgcagc ccttgagcag ctggaaatgg ccaataaaac gcccgaagaa
     4261 cgggcagcag aggcggctaa cccacagact atgaacccac agctcaaaaa taaatttaat
     4321 tacgaagttg aaactaaggc agcacttgta aacaagctaa aagcgggact tgcttttgat
     4381 aaagtgattg ctatgatgaa cgtcagaaaa ggtaaaattc tgatccgccg tattatgccg
     4441 ttcatagccg accagtcgat acgatgggtg gtctggtccg gagttttttg cagtctccag
     4501 actgttgtca agaaagataa agatgatgtc gagggagttc tttatgcact gtatccggaa
     4561 ttcaagaagc atgtgaataa ggcaacattt gagttactcg tctcgatatc ttcagcaatc
     4621 actcttaacg ataaaaagct ggcaggcatc ttctgttccc gattcggcat tctgagcctt
     4681 gtggctctta ttctgagggc tgaagagatt tatgttagcc gcaccgatgc cacgctgtcg
     4741 gaagaaaatc gccagaaatg gagggagttc cttgcccaag tggccagctg tttaaaccgg
     4801 accatacaga atcaaactat tagcgccgct atcgaaagcg acgccatcca acccttgatg
     4861 gatcatttcg agcgcttcaa ggacttgaag cttgacgcct tactagccct aattacggag
     4921 gctcgccacc agatcgacta gggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     4981 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     5041 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc
     5101 tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5161 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5221 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5281 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5341 ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     5401 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     5461 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     5521 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     5581 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     5641 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     5701 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     5761 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggcc agcaaaaggc
     5821 caagaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5881 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5941 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6001 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6061 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6121 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6181 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6241 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6301 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6361 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6421 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6481 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6541 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6601 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6661 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6721 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6781 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6841 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6901 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     6961 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7021 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7081 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7141 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7201 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     7261 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7321 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7381 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7441 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7501 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7561 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     7621 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     7681 attaattcta tattctaaaa acacaaatga tacttctaaa aaaaaat
//
