LOCUS       sequence-35606-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pHD5
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     798..817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
       61 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagaggt ctcctgttgc
      121 cggtgctgtg ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg
      181 atggcggcaa gcaagcgctc gaaacggtgc aggagaccct cgagccaccc atgaccaaaa
      241 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
      301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      421 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      661 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      721 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      781 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgcacc
      841 tctgacttga gcgt
//
