LOCUS       sequence-356076-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette (TU3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Novarina D, Koutsoumpa A, Milias-Argeitis A
  TITLE     A user-friendly and streamlined protocol for CRISPR/Cas9 genome 
            editing in budding yeast.
  JOURNAL   STAR Protoc. 2022 Jun 10;3(2):101358. doi: 
            10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17.
  PUBMED    35712010
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-06-10"; volume: "3(2):101358. doi"; pages: " 
            10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17"
COMMENT     Sequence Label: pYTK-DN2
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(264..1055,1056..1124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     887..906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1125..1229)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1332..1920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1821..1840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata ccgatgactg
       61 cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca ccactgcata
      121 tatggcatat cacaacagtc caactagtgc actgcagtac agcggccgcg attatcaaaa
      181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      241 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      301 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      361 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
      421 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      481 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      541 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      601 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      661 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      721 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      781 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      841 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      901 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      961 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1021 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1081 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtcatg
     1261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1441 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1981 ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcccc tgaattcgca
     2041 tctagaataa atcgcagcca agtgagtgaa tagatgacgc accacggtca gacacggcca
     2101 catcgtatct cacaggagca agcgcgatag gagcactcac acatagtacg gtgatccgct
     2161 gactcctttg cccaaataag acgtgagccc gtctcagatg cacacactgg cttaagatga
     2221 caacgactag agatctatgt gaggatccta actcgagatc gagctgagca tgagacgg
//
