LOCUS       sequence-356077-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette (TU2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Novarina D, Koutsoumpa A, Milias-Argeitis A
  TITLE     A user-friendly and streamlined protocol for CRISPR/Cas9 genome 
            editing in budding yeast.
  JOURNAL   STAR Protoc. 2022 Jun 10;3(2):101358. doi: 
            10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17.
  PUBMED    35712010
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-06-10"; volume: "3(2):101358. doi"; pages: " 
            10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17"
COMMENT     Sequence Label: pYTK-DN3
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(400..1191,1192..1260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1023..1042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1261..1365)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1468..2056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1957..1976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acctaagcct gtgagagaag cagacacccg acagatcaag gcagttacgt ctcaccaaac
       61 cagatgtcaa cacagctaca acgactagag atctatgtga ggatcctaac tcgagatcga
      121 gctggatgtg agacgataaa tcgcagccaa gtgagtgaat agatgacgca ccacggtcag
      181 acacggccac atcgtatctc acaggagcaa gcgcgatagg agcactcaca catagtacgg
      241 tgatccgctg actcctttgc ccaaataaga cgtgagccac tagtgcactg cagtacagcg
      301 gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      541 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
      661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
      721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
      781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
      841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
      901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
      961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1381 agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2101 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtgcggc
     2161 cgcccctgaa ttcgcatcta gaacggggtc atcacggctc atcatgcgcc aaacaaatgt
     2221 gtgcaataca cgctcggatg actgcatgat gaccgcactg actggggaca gcagatcc
//
